Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.