Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures

A statistical approach

Anna Maria Nuutila, Ulrika Kurten, Veli Kauppinen

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)

    Abstract

    The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1-1 sucrose in the medium.

    Original languageEnglish
    Pages (from-to)73 - 77
    Number of pages5
    JournalPlant Cell, Tissue and Organ Culture
    Volume24
    Issue number2
    DOIs
    Publication statusPublished - 1991
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Betula pendula
    callus culture
    somatic embryogenesis
    Betula
    sucrose
    nitrogen
    embryo (animal)
    regression analysis
    experimental design

    Cite this

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    title = "Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach",
    abstract = "The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1-1 sucrose in the medium.",
    author = "Nuutila, {Anna Maria} and Ulrika Kurten and Veli Kauppinen",
    note = "Project code: BIO1020",
    year = "1991",
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    Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures : A statistical approach. / Nuutila, Anna Maria; Kurten, Ulrika; Kauppinen, Veli.

    In: Plant Cell, Tissue and Organ Culture, Vol. 24, No. 2, 1991, p. 73 - 77.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures

    T2 - A statistical approach

    AU - Nuutila, Anna Maria

    AU - Kurten, Ulrika

    AU - Kauppinen, Veli

    N1 - Project code: BIO1020

    PY - 1991

    Y1 - 1991

    N2 - The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1-1 sucrose in the medium.

    AB - The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1-1 sucrose in the medium.

    U2 - 10.1007/BF00039733

    DO - 10.1007/BF00039733

    M3 - Article

    VL - 24

    SP - 73

    EP - 77

    JO - Plant Cell, Tissue and Organ Culture

    JF - Plant Cell, Tissue and Organ Culture

    SN - 0167-6857

    IS - 2

    ER -