Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

Atsushi Iwanaga, Hitoshi Asakawa, Takeshi Fukuma, Momoka Nakamichi, Sakurako Shigematsu, Markus B. Linder, Tetsuya Haruyama (Corresponding Author)

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Abstract

A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.
Original languageEnglish
Pages (from-to)395-399
JournalColloids and Surfaces B: Biointerfaces
Volume84
Issue number2
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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Keywords

  • Self-organized protein layer
  • molecular interface
  • hydrophobin
  • drop-stamp

Cite this

Iwanaga, A., Asakawa, H., Fukuma, T., Nakamichi, M., Shigematsu, S., Linder, M. B., & Haruyama, T. (2011). Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier. Colloids and Surfaces B: Biointerfaces, 84(2), 395-399. https://doi.org/10.1016/j.colsurfb.2011.01.032