Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier

Atsushi Iwanaga, Hitoshi Asakawa, Takeshi Fukuma, Momoka Nakamichi, Sakurako Shigematsu, Markus B. Linder, Tetsuya Haruyama (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.
Original languageEnglish
Pages (from-to)395-399
JournalColloids and Surfaces B: Biointerfaces
Volume84
Issue number2
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

proteins
Proteins
solid surfaces
Adsorption
Frequency modulation
Atomic Force Microscopy
Molecules
Atomic force microscopy
frequency modulation
adsorption
Maltose-Binding Proteins
Maltose
Defects
atomic force microscopy
molecules
defects
organizing
Liquids
Substrates
preparation

Keywords

  • Self-organized protein layer
  • molecular interface
  • hydrophobin
  • drop-stamp

Cite this

Iwanaga, A., Asakawa, H., Fukuma, T., Nakamichi, M., Shigematsu, S., Linder, M. B., & Haruyama, T. (2011). Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier. Colloids and Surfaces B: Biointerfaces, 84(2), 395-399. https://doi.org/10.1016/j.colsurfb.2011.01.032
Iwanaga, Atsushi ; Asakawa, Hitoshi ; Fukuma, Takeshi ; Nakamichi, Momoka ; Shigematsu, Sakurako ; Linder, Markus B. ; Haruyama, Tetsuya. / Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier. In: Colloids and Surfaces B: Biointerfaces. 2011 ; Vol. 84, No. 2. pp. 395-399.
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Iwanaga, A, Asakawa, H, Fukuma, T, Nakamichi, M, Shigematsu, S, Linder, MB & Haruyama, T 2011, 'Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier', Colloids and Surfaces B: Biointerfaces, vol. 84, no. 2, pp. 395-399. https://doi.org/10.1016/j.colsurfb.2011.01.032

Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier. / Iwanaga, Atsushi; Asakawa, Hitoshi; Fukuma, Takeshi; Nakamichi, Momoka; Shigematsu, Sakurako; Linder, Markus B.; Haruyama, Tetsuya (Corresponding Author).

In: Colloids and Surfaces B: Biointerfaces, Vol. 84, No. 2, 2011, p. 395-399.

Research output: Contribution to journalArticleScientificpeer-review

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