A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.
- Self-organized protein layer
- molecular interface
Iwanaga, A., Asakawa, H., Fukuma, T., Nakamichi, M., Shigematsu, S., Linder, M. B., & Haruyama, T. (2011). Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier. Colloids and Surfaces B: Biointerfaces, 84(2), 395-399. https://doi.org/10.1016/j.colsurfb.2011.01.032