Oriented immobilization of antibodies for immunosensing

Inger Vikholm, Martin Albers

Research output: Contribution to journalArticleScientificpeer-review

62 Citations (Scopus)

Abstract

The covalent coupling of antibody fragments to linkers embedded in a monolayer matrix of phosphatidylcholine and cholesterol was examined at the air−water interface by the means of a quartz crystal microbalance, QCM.
Two linkers that bind the free thiols of the Fab‘ fragment were investigated. The nonspecific binding of bovine serum albumin and the specific binding of antigen were also monitored with the QCM.
Standardized radioimmunoassay was used to confirm the immunoreaction and determine binding parameters. The monolayer formation of the linker lipids in the ternary system of phosphatidylcholine and cholesterol was, moreover, demonstrated by film balance studies.
The results demonstrate that the covalent coupling of Fab‘ fragments to linking groups embedded in a phospholipid monolayer matrix is a promising approach to achieve a defined immobilization of antibodies at the sensor surface with high antigen binding effeciency.
Original languageEnglish
Pages (from-to)3865-3872
JournalLangmuir
Volume14
Issue number14
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

Fingerprint

antibodies
immobilization
Antibodies
Monolayers
Immunoglobulin Fab Fragments
Cholesterol
Antigens
Phosphatidylcholines
cholesterol
fragments
antigens
Immunoglobulin Fragments
Quartz crystal microbalances
Phospholipids
radioimmunoassay
Ternary systems
Bovine Serum Albumin
Sulfhydryl Compounds
Lipids
matrices

Cite this

Vikholm, Inger ; Albers, Martin. / Oriented immobilization of antibodies for immunosensing. In: Langmuir. 1998 ; Vol. 14, No. 14. pp. 3865-3872.
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Vikholm, I & Albers, M 1998, 'Oriented immobilization of antibodies for immunosensing', Langmuir, vol. 14, no. 14, pp. 3865-3872. https://doi.org/10.1021/la971412x

Oriented immobilization of antibodies for immunosensing. / Vikholm, Inger; Albers, Martin.

In: Langmuir, Vol. 14, No. 14, 1998, p. 3865-3872.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Albers, Martin

PY - 1998

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