ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs

Marion Weber-Boyvat (Corresponding Author), Thorsten Trimbuch, Saundarya Shah, Jussi Jäntti, Vesa M. Olkkonen, Christian Rosenmund (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)


OSBP-homologous proteins (ORPs, Oshp) are lipid binding/transfer proteins. Several ORP/Oshp localize to membrane contacts between the endoplasmic reticulum (ER) and the plasma membrane, where they mediate lipid transfer or regulate lipid-modifying enzymes. A common way in which they target contacts is by binding to the ER proteins, VAP/Scs2p, while the second membrane is targeted by other interactions with lipids or proteins. We have studied the cross-talk of secretory SNARE proteins and their regulators with ORP/Oshp and VAPA/Scs2p at ER-plasma membrane contact sites in yeast and murine primary neurons. We show that Oshp-Scs2p interactions depend on intact secretory SNARE proteins, especially Sec9p. SNAP-25/Sec9p directly interact with ORP/Osh proteins and their disruption destabilized the ORP/Osh proteins, associated with dysfunction of VAPA/Scs2p. Deleting OSH1-3 in yeast or knocking down ORP2 in primary neurons reduced the oligomerization of VAPA/Scs2p and affected their multiple interactions with SNAREs. These observations reveal a novel cross-talk between the machineries of ER-plasma membrane contact sites and those driving exocytosis.

Original languageEnglish
Pages (from-to)1689-1708
Number of pages20
JournalCellular and Molecular Life Sciences
Issue number4
Publication statusPublished - Feb 2021
MoE publication typeA1 Journal article-refereed


  • Membrane contact site
  • ORP
  • Osh
  • Sec9
  • SNAP-25


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