Overexpressed maltose transporters in laboratory and lager yeasts: Localization and competition with endogenous transporters

Virve Vidgren (Corresponding Author), John Londesborough

    Research output: Contribution to journalArticleScientificpeer-review

    3 Citations (Scopus)

    Abstract

    Plain and fluorescently tagged versions of Agt1, Mtt1 and Malx1 maltose transporters were overexpressed in two laboratory yeasts and one lager yeast. The plain and tagged versions of each transporter supported similar transport activities, indicating that they are similarly trafficked and have similar catalytic activities. When they were expressed under the control of the strong constitutive PGK1 promoter only minor proportions of the fluorescent transporters were associated with the plasma membrane, the rest being found in intracellular structures. Transport activity of each tagged transporter in each host was roughly proportional to the plasma membrane-associated fluorescence. All three transporters were subject to glucose-triggered inactivation when the medium glucose concentration was abruptly raised. Results also suggest competition between endogenous and overexpressed transporters for access to the plasma membrane.

    Original languageEnglish
    Pages (from-to)567-576
    Number of pages10
    JournalYeast
    Volume35
    Issue number10
    DOIs
    Publication statusPublished - 1 Oct 2018
    MoE publication typeNot Eligible

    Keywords

    • Agt1
    • brewer's yeast
    • glucose inactivation
    • maltose transporters
    • Malx1
    • Mtt1
    • plasma membrane

    Fingerprint

    Dive into the research topics of 'Overexpressed maltose transporters in laboratory and lager yeasts: Localization and competition with endogenous transporters'. Together they form a unique fingerprint.

    Cite this