Overexpressed maltose transporters in laboratory and lager yeasts: Localization and competition with endogenous transporters

Virve Vidgren (Corresponding Author), John Londesborough

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Plain and fluorescently tagged versions of Agt1, Mtt1 and Malx1 maltose transporters were overexpressed in two laboratory yeasts and one lager yeast. The plain and tagged versions of each transporter supported similar transport activities, indicating that they are similarly trafficked and have similar catalytic activities. When they were expressed under the control of the strong constitutive PGK1 promoter only minor proportions of the fluorescent transporters were associated with the plasma membrane, the rest being found in intracellular structures. Transport activity of each tagged transporter in each host was roughly proportional to the plasma membrane-associated fluorescence. All three transporters were subject to glucose-triggered inactivation when the medium glucose concentration was abruptly raised. Results also suggest competition between endogenous and overexpressed transporters for access to the plasma membrane.

Original languageEnglish
Pages (from-to)567-576
Number of pages10
JournalYeast
Volume35
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018
MoE publication typeNot Eligible

Fingerprint

Maltose
Beer
Cell membranes
Yeast
Yeasts
Cell Membrane
Glucose
Catalyst activity
Fluorescence

Keywords

  • Agt1
  • brewer's yeast
  • glucose inactivation
  • maltose transporters
  • Malx1
  • Mtt1
  • plasma membrane

Cite this

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Overexpressed maltose transporters in laboratory and lager yeasts : Localization and competition with endogenous transporters. / Vidgren, Virve (Corresponding Author); Londesborough, John.

In: Yeast, Vol. 35, No. 10, 01.10.2018, p. 567-576.

Research output: Contribution to journalArticleScientificpeer-review

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