Overexpression of an endochitinase gene (ThEn-42) in Trichoderma atroviride for increased production of antifungal enzymes and enhanced antagonist action against pathogenic fungi

Shiping Deng, Matteo Lorito, Merja Penttilä, Gary E. Harman

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Trichoderma is one of the most promising biocontrol agents against plant fungal diseases. In this study, a transgenic strain of Trichoderma atroviride was characterized. The transgenic strain contains an endochitinase gene (ThEn-42) driven by the cellulase promoter cbh1 of T. reesei for overexpression of ThEn-42. The culture filtrates of the transformant and the parental strain grown in eight different media were evaluated for chitinase and antifungal enzyme production based on activity gels, protein profiles, and antifungal activities. Results demonstrated that chitinases are important components and synergistic interactions play a key role in the antagonistic action of T. atroviride. Moreover, altering medium nutrient concentration and composition led to enhanced production of antifungal enzymes, a potential strategy for mass production. Two of the culture filtrates contained almost pure endochitinase, and could be excellent commercial sources for this enzyme. Several culture filtrates were highly antifungal. Two filtrates were so effective in biocontrol of a fungal pathogen, Penicillium digitatum, that they not only inhibited spore germination but destroyed the spores completely when 20 μl of culture filtrate (corresponding to approximately 104 μg of total protein) was applied in a total volume of 150 μl (approximately 0.7 mg protein ml-1).

Original languageEnglish
Pages (from-to)81-94
Number of pages14
JournalApplied Biochemistry and Biotechnology
Volume142
Issue number1
DOIs
Publication statusPublished - 1 Jul 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Chitinases
Trichoderma
Fungi
Biocontrol
Enzymes
Genes
Proteins
Spores
Pathogens
Plant Diseases
Nutrients
Cellulase
Mycoses
Penicillium
Gels
Germination
Chemical analysis
Food

Keywords

  • Biocontrol
  • Chitinase
  • Gene expression
  • Synergistic interaction
  • Trichoderma spp

Cite this

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title = "Overexpression of an endochitinase gene (ThEn-42) in Trichoderma atroviride for increased production of antifungal enzymes and enhanced antagonist action against pathogenic fungi",
abstract = "Trichoderma is one of the most promising biocontrol agents against plant fungal diseases. In this study, a transgenic strain of Trichoderma atroviride was characterized. The transgenic strain contains an endochitinase gene (ThEn-42) driven by the cellulase promoter cbh1 of T. reesei for overexpression of ThEn-42. The culture filtrates of the transformant and the parental strain grown in eight different media were evaluated for chitinase and antifungal enzyme production based on activity gels, protein profiles, and antifungal activities. Results demonstrated that chitinases are important components and synergistic interactions play a key role in the antagonistic action of T. atroviride. Moreover, altering medium nutrient concentration and composition led to enhanced production of antifungal enzymes, a potential strategy for mass production. Two of the culture filtrates contained almost pure endochitinase, and could be excellent commercial sources for this enzyme. Several culture filtrates were highly antifungal. Two filtrates were so effective in biocontrol of a fungal pathogen, Penicillium digitatum, that they not only inhibited spore germination but destroyed the spores completely when 20 μl of culture filtrate (corresponding to approximately 104 μg of total protein) was applied in a total volume of 150 μl (approximately 0.7 mg protein ml-1).",
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Overexpression of an endochitinase gene (ThEn-42) in Trichoderma atroviride for increased production of antifungal enzymes and enhanced antagonist action against pathogenic fungi. / Deng, Shiping; Lorito, Matteo; Penttilä, Merja; Harman, Gary E.

In: Applied Biochemistry and Biotechnology, Vol. 142, No. 1, 01.07.2007, p. 81-94.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Overexpression of an endochitinase gene (ThEn-42) in Trichoderma atroviride for increased production of antifungal enzymes and enhanced antagonist action against pathogenic fungi

AU - Deng, Shiping

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AU - Penttilä, Merja

AU - Harman, Gary E.

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