Oxidation of milled wood lignin with laccase, tyrosinase and horseradish peroxidase

Stina Grönqvist (Corresponding Author), Liisa Viikari, Marja-Leena Niku-Paavola, M. Orlandi, C. Canevali, Johanna Buchert

    Research output: Contribution to journalArticleScientificpeer-review

    47 Citations (Scopus)

    Abstract

    In this paper the oxidation of milled wood lignin (MWL), catalysed by three enzymes, i.e. laccase, tyrosinase and horseradish peroxidase (HRP) was studied. The oxidation was followed by measuring the consumption of O2 during laccase and tyrosinase treatment and of H2O2 during HRP treatment. Both laccase and HRP were found to oxidise lignin effectively, whereas the effect of tyrosinase was negligible. The changes in MWL molecular-weight distributions caused in the reactions were analysed by gel permeation chromatography. Both laccase and HRP treatments were found to polymerise MWL. Peroxidase treatment was found to decrease the amount of phenolic hydroxyls in MWL, whereas no such effect could be detected in the laccase-treated sample. Both laccase and HRP treatments were, however, found to increase the amount of conjugated structures in MWL. The formation of phenoxy radicals during the treatments was studied by electron paramagnetic resonance spectroscopy. Phenoxy radicals were detected in both laccase and HRP-treated samples. The amount of the formed phenoxy radicals was found to be essentially constant during the detected time (i.e. 20–120 min after the addition of enzyme).
    Original languageEnglish
    Pages (from-to)489 - 494
    Number of pages6
    JournalApplied Microbiology and Biotechnology
    Volume67
    Issue number4
    DOIs
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

    Keywords

    • lignin
    • milled wood lignin
    • enzymes
    • laccase
    • tyrosinase
    • horseradish peroxidase
    • oxidation

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