PCR detection of Gram-negative brewery contaminants - present state

Riikka Juvonen, Teija Koivula, Auli Haikara

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.
Original languageEnglish
Title of host publicationProceedings of the 29th EBC Congress, Dublin 2003
PublisherFachverlag Hans Carl
Pages1047-1056
ISBN (Print)90-70143-22-4
Publication statusPublished - 2003
MoE publication typeA4 Article in a conference publication
Event29th European Brewery Convention EBC Congress 2003 - Dublin, Ireland
Duration: 17 May 200322 May 2003

Conference

Conference29th European Brewery Convention EBC Congress 2003
CountryIreland
CityDublin
Period17/05/0322/05/03

Keywords

  • accelerated method
  • beer spoilage organism
  • polymerase chain reaction

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  • Cite this

    Juvonen, R., Koivula, T., & Haikara, A. (2003). PCR detection of Gram-negative brewery contaminants - present state. In Proceedings of the 29th EBC Congress, Dublin 2003 (pp. 1047-1056). Fachverlag Hans Carl.