PCR detection of Gram-negative brewery contaminants - present state

Riikka Juvonen; Teija Koivula; Auli Haikara

PCR detection of Gram-negative brewery contaminants - present state

Riikka Juvonen, Teija Koivula, Auli Haikara

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.

Original language	English
Title of host publication	Proceedings of the 29th EBC Congress, Dublin 2003
Publisher	Fachverlag Hans Carl
Pages	1047-1056
ISBN (Print)	90-70143-22-4
Publication status	Published - 2003
MoE publication type	A4 Article in a conference publication
Event	29th European Brewery Convention EBC Congress 2003 - Dublin, Ireland Duration: 17 May 2003 → 22 May 2003

Conference

Conference	29th European Brewery Convention EBC Congress 2003
Country/Territory	Ireland
City	Dublin
Period	17/05/03 → 22/05/03

Keywords

accelerated method
beer spoilage organism
polymerase chain reaction

Cite this

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title = "PCR detection of Gram-negative brewery contaminants - present state",

abstract = "Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.",

keywords = "accelerated method, beer spoilage organism, polymerase chain reaction",

author = "Riikka Juvonen and Teija Koivula and Auli Haikara",

note = "CD-ROM; 29th European Brewery Convention EBC Congress 2003 ; Conference date: 17-05-2003 Through 22-05-2003",

year = "2003",

language = "English",

isbn = "90-70143-22-4",

pages = "1047--1056",

booktitle = "Proceedings of the 29th EBC Congress, Dublin 2003",

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TY - GEN

T1 - PCR detection of Gram-negative brewery contaminants - present state

AU - Juvonen, Riikka

AU - Koivula, Teija

AU - Haikara, Auli

N1 - CD-ROM

PY - 2003

Y1 - 2003

N2 - Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.

AB - Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.

KW - accelerated method

KW - beer spoilage organism

KW - polymerase chain reaction

M3 - Conference article in proceedings

SN - 90-70143-22-4

SP - 1047

EP - 1056

BT - Proceedings of the 29th EBC Congress, Dublin 2003

PB - Fachverlag Hans Carl

T2 - 29th European Brewery Convention EBC Congress 2003

Y2 - 17 May 2003 through 22 May 2003

ER -

PCR detection of Gram-negative brewery contaminants - present state

Abstract

Conference

Keywords

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