PCR detection of Gram-negative brewery contaminants - present state

Riikka Juvonen, Teija Koivula, Auli Haikara

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review


    Gram-negative brewery contaminants, including certain Pectinatus, Megasphaera, Selenomonas, Zymophilus and Zymomonas species, acetic acid bacteria and enterobacteria, constitute a diverse group of spoilage organisms. Routine methods for their detection often lack speed and specificity. In this paper, alternative methods based on PCR are presented, compared and discussed. Standard PCR, real-time PCR and PCR-ELISA assays have been developed for the detection of different species, genera and groups of Gram-negative bacteria in beer and process samples. Most of the assays allow more specific and rapid detection of these contaminants than cultivation. Various detection formats have their specific advantages and disadvantages.
    Original languageEnglish
    Title of host publicationProceedings of the 29th EBC Congress, Dublin 2003
    PublisherFachverlag Hans Carl
    ISBN (Print)90-70143-22-4
    Publication statusPublished - 2003
    MoE publication typeA4 Article in a conference publication
    Event29th European Brewery Convention EBC Congress 2003 - Dublin, Ireland
    Duration: 17 May 200322 May 2003


    Conference29th European Brewery Convention EBC Congress 2003


    • accelerated method
    • beer spoilage organism
    • polymerase chain reaction


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