PCR-ELISA. II

Analysis of Bifidobacterium populations in human faecal samples from a consumption trial with Bifidobacterium lactis Bb-12 and a galacto-oligosaccharide preparation

Erja Malinen, Jaana Mättö, Merja Salmitie, Minna Alander, Maria Saarela, Airi Palva (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

46 Citations (Scopus)

Abstract

A PCR-ELISA method was extended for detection of most common Bifidobacterium species in humans and applied to a feeding trial including administration of Bifidobacterium lactis Bb-12 and galacto-oligosaccharide (GOS)-containing syrup as probiotic and prebiotic preparations, respectively.
For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples. Bifidobacteria were monitored for their fluctuations during and after the feeding trial.
Bifidobacterium longum was the most common species found in the faecal samples, followed by B. adolescentis and B. bifidum. During ingestion of the probiotic B. lactis Bb-12, the strain appeared in the faeces but was absent again one week after finishing of the trial.
The species that were observed in the faecal samples taken prior to the feeding experiments persisted also in samples derived from the pre-feeding and feeding periods. The most consistent change observed was the decrease in the relative amount of B. longum in the test group ingesting either B. lactis Bb-12 alone or in combination with GOS-syrup.
Since the amounts of B. longum increased again in the post-feeding sample with these subjects, it may suggest that to some extent B. lactis Bb-12 is able to transiently replace B. longum.
Original languageEnglish
Pages (from-to)249-258
JournalSystematic and Applied Microbiology
Volume25
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

galactooligosaccharides
Bifidobacterium
Bifidobacterium longum
Oligosaccharides
human population
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Polymerase Chain Reaction
Probiotics
Population
syrups
probiotics
sampling
Prebiotics
Bifidobacterium adolescentis
Oligonucleotide Probes
Bifidobacterium bifidum
Ribosomal DNA
Feces
oligonucleotide probes

Keywords

  • PCR-ELISA
  • Bifidobacterium
  • oligonucleotide probes

Cite this

@article{7ee9e8432e1b431393eb132015090cb8,
title = "PCR-ELISA. II: Analysis of Bifidobacterium populations in human faecal samples from a consumption trial with Bifidobacterium lactis Bb-12 and a galacto-oligosaccharide preparation",
abstract = "A PCR-ELISA method was extended for detection of most common Bifidobacterium species in humans and applied to a feeding trial including administration of Bifidobacterium lactis Bb-12 and galacto-oligosaccharide (GOS)-containing syrup as probiotic and prebiotic preparations, respectively. For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples. Bifidobacteria were monitored for their fluctuations during and after the feeding trial. Bifidobacterium longum was the most common species found in the faecal samples, followed by B. adolescentis and B. bifidum. During ingestion of the probiotic B. lactis Bb-12, the strain appeared in the faeces but was absent again one week after finishing of the trial. The species that were observed in the faecal samples taken prior to the feeding experiments persisted also in samples derived from the pre-feeding and feeding periods. The most consistent change observed was the decrease in the relative amount of B. longum in the test group ingesting either B. lactis Bb-12 alone or in combination with GOS-syrup. Since the amounts of B. longum increased again in the post-feeding sample with these subjects, it may suggest that to some extent B. lactis Bb-12 is able to transiently replace B. longum.",
keywords = "PCR-ELISA, Bifidobacterium, oligonucleotide probes",
author = "Erja Malinen and Jaana M{\"a}tt{\"o} and Merja Salmitie and Minna Alander and Maria Saarela and Airi Palva",
year = "2002",
doi = "10.1078/0723-2020-00117",
language = "English",
volume = "25",
pages = "249--258",
journal = "Systematic and Applied Microbiology",
issn = "0723-2020",
publisher = "Urban und Fischer Verlag Jena",
number = "2",

}

PCR-ELISA. II : Analysis of Bifidobacterium populations in human faecal samples from a consumption trial with Bifidobacterium lactis Bb-12 and a galacto-oligosaccharide preparation. / Malinen, Erja; Mättö, Jaana; Salmitie, Merja; Alander, Minna; Saarela, Maria; Palva, Airi (Corresponding Author).

In: Systematic and Applied Microbiology, Vol. 25, No. 2, 2002, p. 249-258.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - PCR-ELISA. II

T2 - Analysis of Bifidobacterium populations in human faecal samples from a consumption trial with Bifidobacterium lactis Bb-12 and a galacto-oligosaccharide preparation

AU - Malinen, Erja

AU - Mättö, Jaana

AU - Salmitie, Merja

AU - Alander, Minna

AU - Saarela, Maria

AU - Palva, Airi

PY - 2002

Y1 - 2002

N2 - A PCR-ELISA method was extended for detection of most common Bifidobacterium species in humans and applied to a feeding trial including administration of Bifidobacterium lactis Bb-12 and galacto-oligosaccharide (GOS)-containing syrup as probiotic and prebiotic preparations, respectively. For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples. Bifidobacteria were monitored for their fluctuations during and after the feeding trial. Bifidobacterium longum was the most common species found in the faecal samples, followed by B. adolescentis and B. bifidum. During ingestion of the probiotic B. lactis Bb-12, the strain appeared in the faeces but was absent again one week after finishing of the trial. The species that were observed in the faecal samples taken prior to the feeding experiments persisted also in samples derived from the pre-feeding and feeding periods. The most consistent change observed was the decrease in the relative amount of B. longum in the test group ingesting either B. lactis Bb-12 alone or in combination with GOS-syrup. Since the amounts of B. longum increased again in the post-feeding sample with these subjects, it may suggest that to some extent B. lactis Bb-12 is able to transiently replace B. longum.

AB - A PCR-ELISA method was extended for detection of most common Bifidobacterium species in humans and applied to a feeding trial including administration of Bifidobacterium lactis Bb-12 and galacto-oligosaccharide (GOS)-containing syrup as probiotic and prebiotic preparations, respectively. For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples. Bifidobacteria were monitored for their fluctuations during and after the feeding trial. Bifidobacterium longum was the most common species found in the faecal samples, followed by B. adolescentis and B. bifidum. During ingestion of the probiotic B. lactis Bb-12, the strain appeared in the faeces but was absent again one week after finishing of the trial. The species that were observed in the faecal samples taken prior to the feeding experiments persisted also in samples derived from the pre-feeding and feeding periods. The most consistent change observed was the decrease in the relative amount of B. longum in the test group ingesting either B. lactis Bb-12 alone or in combination with GOS-syrup. Since the amounts of B. longum increased again in the post-feeding sample with these subjects, it may suggest that to some extent B. lactis Bb-12 is able to transiently replace B. longum.

KW - PCR-ELISA

KW - Bifidobacterium

KW - oligonucleotide probes

U2 - 10.1078/0723-2020-00117

DO - 10.1078/0723-2020-00117

M3 - Article

VL - 25

SP - 249

EP - 258

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

SN - 0723-2020

IS - 2

ER -