Penicillium subrubescens adapts its enzyme production to the composition of plant biomass

Adiphol Dilokpimol; Mao Peng; Marcos Di Falco; Thomas Chin A Woeng; Rosa M W Hegi; Zoraide Granchi; Adrian Tsang; Kristiina S Hildén; Miia R Mäkelä; Ronald P de Vries

doi:10.1016/j.biortech.2020.123477

Penicillium subrubescens adapts its enzyme production to the composition of plant biomass

Adiphol Dilokpimol, Mao Peng, Marcos Di Falco, Thomas Chin A Woeng, Rosa M W Hegi, Zoraide Granchi, Adrian Tsang, Kristiina S Hildén, Miia R Mäkelä, Ronald P de Vries

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15 Citations (Scopus)

Abstract

Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison to other Penicillium species. Here we used exoproteome and transcriptome analysis to demonstrate the versatile plant biomass degradation mechanism by P. subrubescens during growth on wheat bran and sugar beet pulp. On wheat bran P. subrubescens degraded xylan main chain and side residues from Day 2 of cultivation, whereas it started to degrade side chains of pectin in sugar beet pulp prior to attacking the main chain on Day 3. In addition, on Day 3 the cellulolytic enzymes were highly increased. Our results confirm that P. subrubescens adapts its enzyme production to the available plant biomass and is a promising new fungal cell factory for the production of CAZymes.

Original language	English
Article number	123477
Journal	Bioresource Technology
Volume	311
DOIs	https://doi.org/10.1016/j.biortech.2020.123477
Publication status	Published - Sept 2020
MoE publication type	A1 Journal article-refereed

Keywords

CAZyme
Exoproteome
Penicillium subrubescens
Plant biomass
Transcriptome

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10.1016/j.biortech.2020.123477

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title = "Penicillium subrubescens adapts its enzyme production to the composition of plant biomass",

abstract = "Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison to other Penicillium species. Here we used exoproteome and transcriptome analysis to demonstrate the versatile plant biomass degradation mechanism by P. subrubescens during growth on wheat bran and sugar beet pulp. On wheat bran P. subrubescens degraded xylan main chain and side residues from Day 2 of cultivation, whereas it started to degrade side chains of pectin in sugar beet pulp prior to attacking the main chain on Day 3. In addition, on Day 3 the cellulolytic enzymes were highly increased. Our results confirm that P. subrubescens adapts its enzyme production to the available plant biomass and is a promising new fungal cell factory for the production of CAZymes.",

keywords = "CAZyme, Exoproteome, Penicillium subrubescens, Plant biomass, Transcriptome",

author = "Adiphol Dilokpimol and Mao Peng and {Di Falco}, Marcos and {Chin A Woeng}, Thomas and Hegi, {Rosa M W} and Zoraide Granchi and Adrian Tsang and Hild{\'e}n, {Kristiina S} and M{\"a}kel{\"a}, {Miia R} and {de Vries}, {Ronald P}",

note = "Funding Information: This work was supported by the European Union, Grant agreement no: 613868 (OPTIBIOCAT), Genome Canada and G?nome Qu?bec. Mass spectrometry proteomics analysis was carried out at the Centre for Biological Applications of Mass Spectrometry (CBAMS) at Concordia University, Montreal, Canada. ",

year = "2020",

month = sep,

doi = "10.1016/j.biortech.2020.123477",

language = "English",

volume = "311",

journal = "Bioresource Technology",

issn = "0960-8524",

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AU - Dilokpimol, Adiphol

AU - Peng, Mao

AU - Di Falco, Marcos

AU - Chin A Woeng, Thomas

AU - Hegi, Rosa M W

AU - Granchi, Zoraide

AU - Tsang, Adrian

AU - Hildén, Kristiina S

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

N1 - Funding Information: This work was supported by the European Union, Grant agreement no: 613868 (OPTIBIOCAT), Genome Canada and G?nome Qu?bec. Mass spectrometry proteomics analysis was carried out at the Centre for Biological Applications of Mass Spectrometry (CBAMS) at Concordia University, Montreal, Canada.

PY - 2020/9

Y1 - 2020/9

N2 - Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison to other Penicillium species. Here we used exoproteome and transcriptome analysis to demonstrate the versatile plant biomass degradation mechanism by P. subrubescens during growth on wheat bran and sugar beet pulp. On wheat bran P. subrubescens degraded xylan main chain and side residues from Day 2 of cultivation, whereas it started to degrade side chains of pectin in sugar beet pulp prior to attacking the main chain on Day 3. In addition, on Day 3 the cellulolytic enzymes were highly increased. Our results confirm that P. subrubescens adapts its enzyme production to the available plant biomass and is a promising new fungal cell factory for the production of CAZymes.

AB - Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison to other Penicillium species. Here we used exoproteome and transcriptome analysis to demonstrate the versatile plant biomass degradation mechanism by P. subrubescens during growth on wheat bran and sugar beet pulp. On wheat bran P. subrubescens degraded xylan main chain and side residues from Day 2 of cultivation, whereas it started to degrade side chains of pectin in sugar beet pulp prior to attacking the main chain on Day 3. In addition, on Day 3 the cellulolytic enzymes were highly increased. Our results confirm that P. subrubescens adapts its enzyme production to the available plant biomass and is a promising new fungal cell factory for the production of CAZymes.

KW - CAZyme

KW - Exoproteome

KW - Penicillium subrubescens

KW - Plant biomass

KW - Transcriptome

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DO - 10.1016/j.biortech.2020.123477

M3 - Article

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SN - 0960-8524

VL - 311

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 123477

ER -

Penicillium subrubescens adapts its enzyme production to the composition of plant biomass

Abstract

Keywords

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