Performance and flow dynamics studies of polymeric optofluidic SERS sensors

Sanna Uusitalo (Corresponding Author), Jussi Hiltunen, Pentti Karioja, Samuli Siitonen, Ville Kontturi, Risto Myllylä, Matti Kinnunen, Igor Meglinski

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

We present a polymer-based optofluidic surface enhanced Raman scattering chip for biomolecule detection, serving as a disposable sensorchoice with cost-effective production. The SERS substrate is fabricated by using industrial roll-to-roll UV-nanoimprinting equipment andintegrated with adhesive-based polymeric microfluidics. The functioning of the SERS detection on-chip is confirmed and the effect of thepolymer lid on the obtainable Raman spectra is analysed. Rhodamine 6G is used as a model analyte to demonstrate continuous flowmeasurements on a planar SERS substrate in a microchannel. The relation between the temporal response of the sensors and sample flowdynamics is studied with varied flow velocities, using SERS and fluorescence detection. The response time of the surface-dependent SERSsignal is longer than the response time of the fluorescence signal of the bulk flow. This observation revealed the effect of convection on thetemporal SERS responses at 25 [...]l/min to 1000 [...]l/min flow velocities. The diffusion of analyte molecules from the bulk concentration intothe sensing surface induces about a 40-second lag time in the SERS detection. This lag time, and its rising trend with slower flow velocities, has to be taken into account in future trials of the optofluidic SERS sensor, with active analyte binding on the sensing surface.
Original languageEnglish
Article number15043
Number of pages8
JournalJournal of the European Optical Society: Rapid Publications
Volume10
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • biosensor
  • continuous
  • flow
  • optofluidic
  • polymer
  • SERS

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