Performance and penetration of laccase and ABTS inks on various printing substrates

Katriina Matilainen (Corresponding Author), Tiina Hämäläinen, Anne Savolainen, Thea Sipiläinen-Malm, Jouko Peltonen, Tomi Erho, Maria Smolander

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Introduction of an enzyme and a colour-forming reagent into paper enables the development of an authenticity indicator. The purpose of this work was to study the performance of Trametes versicolor laccase, TvL, and ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt, in various printing substrates when printed with inkjet. The printing substrates included pre-coated mechanical paper additionally coated with PVA, silica and latex. The focus was on the bioanalytical performance and ink penetration. The setting of the printed TvL and ABTS ink was studied visually, with optical and confocal microscopy and with a so-called tape laminating technique. Technical properties of the printing substrates and effect of the surface chemistry were discussed and related to the bioanalytical properties.

TvL activity persisted well during the printing. The best colour response was attained using the PVA-coated base paper. The film-forming ability of the PVA was found to be the main contributor to the colour reaction. The uniform, dense and non-porous PVA layer retains the ABTS and TVL molecules on top of the printing substrate. The high local ink concentration on the PVA coating layer combined with the absorptive paper substrate suggests that the PVA film acts as a filtering layer which retains TvL and ABTS molecules in the coating layer but allows most of the ink solvents to penetrate into the paper structure. TvL and ABTS molecules are also trapped in the PVA polymer network due to swelling effect of water. Electrostatic attractions between the PVA and ABTS and TvL molecules do not contribute to the colour reaction.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume90
Issue number1
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Printing
Ink
inks
printing
penetration
Color
Substrates
color
Molecules
molecules
coatings
Laminating
Coatings
Trametes
Confocal microscopy
sulfonic acid
latex
Surface chemistry
Latexes

Keywords

  • Bioactive paper
  • enzyme
  • inkjet
  • printing substrate
  • ink penetration

Cite this

Matilainen, Katriina ; Hämäläinen, Tiina ; Savolainen, Anne ; Sipiläinen-Malm, Thea ; Peltonen, Jouko ; Erho, Tomi ; Smolander, Maria. / Performance and penetration of laccase and ABTS inks on various printing substrates. In: Colloids and Surfaces B: Biointerfaces. 2012 ; Vol. 90, No. 1. pp. 119-128.
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Performance and penetration of laccase and ABTS inks on various printing substrates. / Matilainen, Katriina (Corresponding Author); Hämäläinen, Tiina; Savolainen, Anne; Sipiläinen-Malm, Thea; Peltonen, Jouko; Erho, Tomi; Smolander, Maria.

In: Colloids and Surfaces B: Biointerfaces, Vol. 90, No. 1, 2012, p. 119-128.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Performance and penetration of laccase and ABTS inks on various printing substrates

AU - Matilainen, Katriina

AU - Hämäläinen, Tiina

AU - Savolainen, Anne

AU - Sipiläinen-Malm, Thea

AU - Peltonen, Jouko

AU - Erho, Tomi

AU - Smolander, Maria

N1 - Project code: 31738

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N2 - Introduction of an enzyme and a colour-forming reagent into paper enables the development of an authenticity indicator. The purpose of this work was to study the performance of Trametes versicolor laccase, TvL, and ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt, in various printing substrates when printed with inkjet. The printing substrates included pre-coated mechanical paper additionally coated with PVA, silica and latex. The focus was on the bioanalytical performance and ink penetration. The setting of the printed TvL and ABTS ink was studied visually, with optical and confocal microscopy and with a so-called tape laminating technique. Technical properties of the printing substrates and effect of the surface chemistry were discussed and related to the bioanalytical properties.TvL activity persisted well during the printing. The best colour response was attained using the PVA-coated base paper. The film-forming ability of the PVA was found to be the main contributor to the colour reaction. The uniform, dense and non-porous PVA layer retains the ABTS and TVL molecules on top of the printing substrate. The high local ink concentration on the PVA coating layer combined with the absorptive paper substrate suggests that the PVA film acts as a filtering layer which retains TvL and ABTS molecules in the coating layer but allows most of the ink solvents to penetrate into the paper structure. TvL and ABTS molecules are also trapped in the PVA polymer network due to swelling effect of water. Electrostatic attractions between the PVA and ABTS and TvL molecules do not contribute to the colour reaction.

AB - Introduction of an enzyme and a colour-forming reagent into paper enables the development of an authenticity indicator. The purpose of this work was to study the performance of Trametes versicolor laccase, TvL, and ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt, in various printing substrates when printed with inkjet. The printing substrates included pre-coated mechanical paper additionally coated with PVA, silica and latex. The focus was on the bioanalytical performance and ink penetration. The setting of the printed TvL and ABTS ink was studied visually, with optical and confocal microscopy and with a so-called tape laminating technique. Technical properties of the printing substrates and effect of the surface chemistry were discussed and related to the bioanalytical properties.TvL activity persisted well during the printing. The best colour response was attained using the PVA-coated base paper. The film-forming ability of the PVA was found to be the main contributor to the colour reaction. The uniform, dense and non-porous PVA layer retains the ABTS and TVL molecules on top of the printing substrate. The high local ink concentration on the PVA coating layer combined with the absorptive paper substrate suggests that the PVA film acts as a filtering layer which retains TvL and ABTS molecules in the coating layer but allows most of the ink solvents to penetrate into the paper structure. TvL and ABTS molecules are also trapped in the PVA polymer network due to swelling effect of water. Electrostatic attractions between the PVA and ABTS and TvL molecules do not contribute to the colour reaction.

KW - Bioactive paper

KW - enzyme

KW - inkjet

KW - printing substrate

KW - ink penetration

U2 - 10.1016/j.colsurfb.2011.10.015

DO - 10.1016/j.colsurfb.2011.10.015

M3 - Article

VL - 90

SP - 119

EP - 128

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 1

ER -