Performance evaluation of disinfectant formulations using poloxamer-hydrogel biofilm-contructs

Gun Wirtanen, Satu Salo, D. Allison, Tiina Mattila-Sandholm, P. Gilbert (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Poloxamer F127 is a di‐block co‐polymer of polyoxyethylene and polyoxypropylene. Aqueous solutions show thermo‐reversible gelation, being liquid at temperatures < 15 °C and robust gels at temperatures > 15 °C. Chilled poloxamer solutions (30% w/v) were inoculated with approximately 10+−5 cfu ml−1 of stationary phase cultures of Pseudomonas aeruginosa, Ps. fluorescens, Pantoea agglomerans, Micrococcus luteus, Staphylococcus epidermidis, Bacillus subtilis or Listeria innocua. Drops (200 μl) of the inoculated poloxamers were placed on stainless steel coupons held in Petri dishes containing moistened cotton wool and incubated at 30 °C for 5 h. All strains grew well giving between 106–7 cfu ml−1 at 5–6 h. The cultured gels were readily applied to tests of biocide effectiveness as the stainless steel coupons could be removed and flooded with biocide solution for fixed exposure times. Provided that the temperature of the biocide solutions was > 15 °C, the integrity of the gels could be maintained during exposure. After exposure, the gels and their supports were removed to separate tubes containing neutralizer solution (< 15 °C). The gels rapidly dispersed within 5 min to ensure a complete recovery of the sample population. Biofilm‐constructs and cell suspensions (107 cfu ml−1) were exposed to four commercial disinfectant formulations, based on hypochlorite, alcohol, hydrogen peroxide and a tenside, at recommended use levels. Cell suspensions, in the presence of bovine serum albumen (BSA; 0·03% w/v), were subject to a > 5‐log kill within 5 min while the killing effected against the biofilm‐constructs varied between 0·4 and 2‐log reductions. The results indicate a high degree of reproducibility between replicate samples, with patterns of susceptibility varying both as a function of organism, biocide type and concentration. The experiments strongly support the view that poloxamer‐constructs are suitable for application in trials and testing of disinfectant formulations.
Original languageEnglish
Pages (from-to)965-971
JournalJournal of Applied Microbiology
Volume85
Issue number6
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

Fingerprint

Poloxamer
Disinfectants
Hydrogel
Biofilms
Gels
UCON 50-HB-5100
Stainless Steel
Suspensions
Pantoea
Micrococcus luteus
Hypochlorous Acid
Listeria
Temperature
Staphylococcus epidermidis
Wool
Bacillus subtilis
Surface-Active Agents
Pseudomonas aeruginosa
Hydrogen Peroxide
Alcohols

Cite this

Wirtanen, Gun ; Salo, Satu ; Allison, D. ; Mattila-Sandholm, Tiina ; Gilbert, P. / Performance evaluation of disinfectant formulations using poloxamer-hydrogel biofilm-contructs. In: Journal of Applied Microbiology. 1998 ; Vol. 85, No. 6. pp. 965-971.
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abstract = "Poloxamer F127 is a di‐block co‐polymer of polyoxyethylene and polyoxypropylene. Aqueous solutions show thermo‐reversible gelation, being liquid at temperatures < 15 °C and robust gels at temperatures > 15 °C. Chilled poloxamer solutions (30{\%} w/v) were inoculated with approximately 10+−5 cfu ml−1 of stationary phase cultures of Pseudomonas aeruginosa, Ps. fluorescens, Pantoea agglomerans, Micrococcus luteus, Staphylococcus epidermidis, Bacillus subtilis or Listeria innocua. Drops (200 μl) of the inoculated poloxamers were placed on stainless steel coupons held in Petri dishes containing moistened cotton wool and incubated at 30 °C for 5 h. All strains grew well giving between 106–7 cfu ml−1 at 5–6 h. The cultured gels were readily applied to tests of biocide effectiveness as the stainless steel coupons could be removed and flooded with biocide solution for fixed exposure times. Provided that the temperature of the biocide solutions was > 15 °C, the integrity of the gels could be maintained during exposure. After exposure, the gels and their supports were removed to separate tubes containing neutralizer solution (< 15 °C). The gels rapidly dispersed within 5 min to ensure a complete recovery of the sample population. Biofilm‐constructs and cell suspensions (107 cfu ml−1) were exposed to four commercial disinfectant formulations, based on hypochlorite, alcohol, hydrogen peroxide and a tenside, at recommended use levels. Cell suspensions, in the presence of bovine serum albumen (BSA; 0·03{\%} w/v), were subject to a > 5‐log kill within 5 min while the killing effected against the biofilm‐constructs varied between 0·4 and 2‐log reductions. The results indicate a high degree of reproducibility between replicate samples, with patterns of susceptibility varying both as a function of organism, biocide type and concentration. The experiments strongly support the view that poloxamer‐constructs are suitable for application in trials and testing of disinfectant formulations.",
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Performance evaluation of disinfectant formulations using poloxamer-hydrogel biofilm-contructs. / Wirtanen, Gun; Salo, Satu; Allison, D.; Mattila-Sandholm, Tiina; Gilbert, P. (Corresponding Author).

In: Journal of Applied Microbiology, Vol. 85, No. 6, 1998, p. 965-971.

Research output: Contribution to journalArticleScientificpeer-review

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N2 - Poloxamer F127 is a di‐block co‐polymer of polyoxyethylene and polyoxypropylene. Aqueous solutions show thermo‐reversible gelation, being liquid at temperatures < 15 °C and robust gels at temperatures > 15 °C. Chilled poloxamer solutions (30% w/v) were inoculated with approximately 10+−5 cfu ml−1 of stationary phase cultures of Pseudomonas aeruginosa, Ps. fluorescens, Pantoea agglomerans, Micrococcus luteus, Staphylococcus epidermidis, Bacillus subtilis or Listeria innocua. Drops (200 μl) of the inoculated poloxamers were placed on stainless steel coupons held in Petri dishes containing moistened cotton wool and incubated at 30 °C for 5 h. All strains grew well giving between 106–7 cfu ml−1 at 5–6 h. The cultured gels were readily applied to tests of biocide effectiveness as the stainless steel coupons could be removed and flooded with biocide solution for fixed exposure times. Provided that the temperature of the biocide solutions was > 15 °C, the integrity of the gels could be maintained during exposure. After exposure, the gels and their supports were removed to separate tubes containing neutralizer solution (< 15 °C). The gels rapidly dispersed within 5 min to ensure a complete recovery of the sample population. Biofilm‐constructs and cell suspensions (107 cfu ml−1) were exposed to four commercial disinfectant formulations, based on hypochlorite, alcohol, hydrogen peroxide and a tenside, at recommended use levels. Cell suspensions, in the presence of bovine serum albumen (BSA; 0·03% w/v), were subject to a > 5‐log kill within 5 min while the killing effected against the biofilm‐constructs varied between 0·4 and 2‐log reductions. The results indicate a high degree of reproducibility between replicate samples, with patterns of susceptibility varying both as a function of organism, biocide type and concentration. The experiments strongly support the view that poloxamer‐constructs are suitable for application in trials and testing of disinfectant formulations.

AB - Poloxamer F127 is a di‐block co‐polymer of polyoxyethylene and polyoxypropylene. Aqueous solutions show thermo‐reversible gelation, being liquid at temperatures < 15 °C and robust gels at temperatures > 15 °C. Chilled poloxamer solutions (30% w/v) were inoculated with approximately 10+−5 cfu ml−1 of stationary phase cultures of Pseudomonas aeruginosa, Ps. fluorescens, Pantoea agglomerans, Micrococcus luteus, Staphylococcus epidermidis, Bacillus subtilis or Listeria innocua. Drops (200 μl) of the inoculated poloxamers were placed on stainless steel coupons held in Petri dishes containing moistened cotton wool and incubated at 30 °C for 5 h. All strains grew well giving between 106–7 cfu ml−1 at 5–6 h. The cultured gels were readily applied to tests of biocide effectiveness as the stainless steel coupons could be removed and flooded with biocide solution for fixed exposure times. Provided that the temperature of the biocide solutions was > 15 °C, the integrity of the gels could be maintained during exposure. After exposure, the gels and their supports were removed to separate tubes containing neutralizer solution (< 15 °C). The gels rapidly dispersed within 5 min to ensure a complete recovery of the sample population. Biofilm‐constructs and cell suspensions (107 cfu ml−1) were exposed to four commercial disinfectant formulations, based on hypochlorite, alcohol, hydrogen peroxide and a tenside, at recommended use levels. Cell suspensions, in the presence of bovine serum albumen (BSA; 0·03% w/v), were subject to a > 5‐log kill within 5 min while the killing effected against the biofilm‐constructs varied between 0·4 and 2‐log reductions. The results indicate a high degree of reproducibility between replicate samples, with patterns of susceptibility varying both as a function of organism, biocide type and concentration. The experiments strongly support the view that poloxamer‐constructs are suitable for application in trials and testing of disinfectant formulations.

U2 - 10.1111/j.1365-2672.1998.tb05260.x

DO - 10.1111/j.1365-2672.1998.tb05260.x

M3 - Article

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JO - Journal of Applied Microbiology

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