Permanent jejunal fistula

Promising method for obtaining small intestinal chyme without disturbing intestinal function

Jaana Harmoinen, Jaana Mättö, Minna Rinkinen, Maria Wilsson-Rahmberg, Elias Westermarck

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

Accurate information on changes in small intestinal microflora in dogs is rather limited because of difficulties in obtaining samples of small intestinal chyme. In the study reported here, intussuscepted nipple valves were surgically placed into the jejunum of seven laboratory beagles to obtain intestinal juice samples. The influence of the fistula on intestinal motility was determined by use of barium-impregnated polyethylene spheres (BIPS) and on microflora by use of bacterial culturing. The BIPS were fed two weeks before surgery and again five weeks after surgery. Bacterial samples were collected before (fecal samples), during (small intestinal samples) and 11 weeks after surgery. There were no surgical complications, and the animals tolerated the fistula well. Mean orocolic transit percentage was 93% before and 83% after surgery, and notable changes in gastrointestinal motility were not seen, except in one dog. The surgery did not markedly alter the bacterial flora in feces. Microflora did change in small intestinal samples; however, methodologic factors may explain most of these differences. In conclusion, the nipple valve is a promising method that creates easy and safe long-term access to the jejunum and appears not to have an influence on intestinal function.
Original languageEnglish
Pages (from-to)252-256
Number of pages5
JournalComparative Medicine
Volume51
Issue number3
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

fistula
Surgery
Fistula
surgery
Gastrointestinal Motility
Nipples
Polyethylene
Jejunum
Barium
gastrointestinal motility
barium
jejunum
sampling
polyethylene
Dogs
methodology
microorganisms
Feces
dogs
Beagle

Cite this

Harmoinen, J., Mättö, J., Rinkinen, M., Wilsson-Rahmberg, M., & Westermarck, E. (2001). Permanent jejunal fistula: Promising method for obtaining small intestinal chyme without disturbing intestinal function. Comparative Medicine, 51(3), 252-256.
Harmoinen, Jaana ; Mättö, Jaana ; Rinkinen, Minna ; Wilsson-Rahmberg, Maria ; Westermarck, Elias. / Permanent jejunal fistula : Promising method for obtaining small intestinal chyme without disturbing intestinal function. In: Comparative Medicine. 2001 ; Vol. 51, No. 3. pp. 252-256.
@article{e27803b5ad3446fe89ebac53c3b40520,
title = "Permanent jejunal fistula: Promising method for obtaining small intestinal chyme without disturbing intestinal function",
abstract = "Accurate information on changes in small intestinal microflora in dogs is rather limited because of difficulties in obtaining samples of small intestinal chyme. In the study reported here, intussuscepted nipple valves were surgically placed into the jejunum of seven laboratory beagles to obtain intestinal juice samples. The influence of the fistula on intestinal motility was determined by use of barium-impregnated polyethylene spheres (BIPS) and on microflora by use of bacterial culturing. The BIPS were fed two weeks before surgery and again five weeks after surgery. Bacterial samples were collected before (fecal samples), during (small intestinal samples) and 11 weeks after surgery. There were no surgical complications, and the animals tolerated the fistula well. Mean orocolic transit percentage was 93{\%} before and 83{\%} after surgery, and notable changes in gastrointestinal motility were not seen, except in one dog. The surgery did not markedly alter the bacterial flora in feces. Microflora did change in small intestinal samples; however, methodologic factors may explain most of these differences. In conclusion, the nipple valve is a promising method that creates easy and safe long-term access to the jejunum and appears not to have an influence on intestinal function.",
author = "Jaana Harmoinen and Jaana M{\"a}tt{\"o} and Minna Rinkinen and Maria Wilsson-Rahmberg and Elias Westermarck",
year = "2001",
language = "English",
volume = "51",
pages = "252--256",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "3",

}

Harmoinen, J, Mättö, J, Rinkinen, M, Wilsson-Rahmberg, M & Westermarck, E 2001, 'Permanent jejunal fistula: Promising method for obtaining small intestinal chyme without disturbing intestinal function', Comparative Medicine, vol. 51, no. 3, pp. 252-256.

Permanent jejunal fistula : Promising method for obtaining small intestinal chyme without disturbing intestinal function. / Harmoinen, Jaana; Mättö, Jaana; Rinkinen, Minna; Wilsson-Rahmberg, Maria; Westermarck, Elias.

In: Comparative Medicine, Vol. 51, No. 3, 2001, p. 252-256.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Permanent jejunal fistula

T2 - Promising method for obtaining small intestinal chyme without disturbing intestinal function

AU - Harmoinen, Jaana

AU - Mättö, Jaana

AU - Rinkinen, Minna

AU - Wilsson-Rahmberg, Maria

AU - Westermarck, Elias

PY - 2001

Y1 - 2001

N2 - Accurate information on changes in small intestinal microflora in dogs is rather limited because of difficulties in obtaining samples of small intestinal chyme. In the study reported here, intussuscepted nipple valves were surgically placed into the jejunum of seven laboratory beagles to obtain intestinal juice samples. The influence of the fistula on intestinal motility was determined by use of barium-impregnated polyethylene spheres (BIPS) and on microflora by use of bacterial culturing. The BIPS were fed two weeks before surgery and again five weeks after surgery. Bacterial samples were collected before (fecal samples), during (small intestinal samples) and 11 weeks after surgery. There were no surgical complications, and the animals tolerated the fistula well. Mean orocolic transit percentage was 93% before and 83% after surgery, and notable changes in gastrointestinal motility were not seen, except in one dog. The surgery did not markedly alter the bacterial flora in feces. Microflora did change in small intestinal samples; however, methodologic factors may explain most of these differences. In conclusion, the nipple valve is a promising method that creates easy and safe long-term access to the jejunum and appears not to have an influence on intestinal function.

AB - Accurate information on changes in small intestinal microflora in dogs is rather limited because of difficulties in obtaining samples of small intestinal chyme. In the study reported here, intussuscepted nipple valves were surgically placed into the jejunum of seven laboratory beagles to obtain intestinal juice samples. The influence of the fistula on intestinal motility was determined by use of barium-impregnated polyethylene spheres (BIPS) and on microflora by use of bacterial culturing. The BIPS were fed two weeks before surgery and again five weeks after surgery. Bacterial samples were collected before (fecal samples), during (small intestinal samples) and 11 weeks after surgery. There were no surgical complications, and the animals tolerated the fistula well. Mean orocolic transit percentage was 93% before and 83% after surgery, and notable changes in gastrointestinal motility were not seen, except in one dog. The surgery did not markedly alter the bacterial flora in feces. Microflora did change in small intestinal samples; however, methodologic factors may explain most of these differences. In conclusion, the nipple valve is a promising method that creates easy and safe long-term access to the jejunum and appears not to have an influence on intestinal function.

M3 - Article

VL - 51

SP - 252

EP - 256

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 3

ER -

Harmoinen J, Mättö J, Rinkinen M, Wilsson-Rahmberg M, Westermarck E. Permanent jejunal fistula: Promising method for obtaining small intestinal chyme without disturbing intestinal function. Comparative Medicine. 2001;51(3):252-256.