Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption

Minna Alander, Reetta Satokari, Riitta Korpela, Maija Saxelin, Terttu Vilpponen-Salmela, Tiina Mattila-Sandholm, Atte von Wright (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

380 Citations (Scopus)

Abstract

Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.
Original languageEnglish
Pages (from-to)351-354
Number of pages4
JournalApplied and Environmental Microbiology
Volume65
Issue number1
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Lactobacillus rhamnosus
probiotics
Probiotics
mucosa
mouth
Mucous Membrane
colonization
persistence
fermentation
Biopsy
biopsy
Intestinal Mucosa
Lactose
Fermentation
Volunteers
intestinal mucosa
digestive system diseases
whey
sampling
Polymerase Chain Reaction

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Alander, Minna ; Satokari, Reetta ; Korpela, Riitta ; Saxelin, Maija ; Vilpponen-Salmela, Terttu ; Mattila-Sandholm, Tiina ; Wright, Atte von. / Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. In: Applied and Environmental Microbiology. 1999 ; Vol. 65, No. 1. pp. 351-354.
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abstract = "Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.",
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Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. / Alander, Minna; Satokari, Reetta; Korpela, Riitta; Saxelin, Maija; Vilpponen-Salmela, Terttu; Mattila-Sandholm, Tiina; Wright, Atte von (Corresponding Author).

In: Applied and Environmental Microbiology, Vol. 65, No. 1, 1999, p. 351-354.

Research output: Contribution to journalArticleScientificpeer-review

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AB - Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.

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