Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres

Leena Ahonen, Anu Kukkonen, Jeroen Pouwels, Margaret A. Bolton, Christopher D. Jingle, P. Todd Stukenberg, Marko Kallio (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Incenp is an essential mitotic protein that, together with Aurora B, Survivin, and Borealin, forms the core of the chromosomal passenger protein complex (CPC). The CPC regulates various mitotic processes and functions to maintain genomic stability. The proper subcellular localization of the CPC and its full catalytic activity require the presence of each core subunit in the complex. We have investigated the mitotic tasks of the CPC using a function blocking antibody against Incenp microinjected into cells at different mitotic phases. This method allowed temporal analysis of CPC functions without perturbation of complex assembly or activity prior to injection. We have also studied the dynamic properties of Incenp and Aurora B using fusion protein photobleaching. We found that in early mitotic cells, Incenp and Aurora B exhibit dynamic turnover at centromeres, which is prevented by the anti-Incenp antibody. In these cells, the loss of centromeric CPC turnover is accompanied by forced mitotic exit without the execution of cytokinesis. Introduction of anti-Incenp antibody into early anaphase cells causes abnormalities in sister chromatid separation through defects in anaphase spindle functions. In summary, our data uncovers new mitotic roles for the CPC in anaphase and proposes that CPC turnover at centromeres modulates spindle assembly checkpoint signaling.
Original languageEnglish
Pages (from-to)71-84
JournalChromosoma
Volume118
Issue number1
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Anaphase
Chromatids
Centromere
Proteins
Anti-Idiotypic Antibodies
M Phase Cell Cycle Checkpoints
Photobleaching
Blocking Antibodies
Cytokinesis
Genomic Instability

Keywords

  • incenp
  • mitotic proteins
  • CPC
  • anaphase
  • genomic instability

Cite this

Ahonen, L., Kukkonen, A., Pouwels, J., Bolton, M. A., Jingle, C. D., Stukenberg, P. T., & Kallio, M. (2009). Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres. Chromosoma, 118(1), 71-84. https://doi.org/10.1007/s00412-008-0178-0
Ahonen, Leena ; Kukkonen, Anu ; Pouwels, Jeroen ; Bolton, Margaret A. ; Jingle, Christopher D. ; Stukenberg, P. Todd ; Kallio, Marko. / Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres. In: Chromosoma. 2009 ; Vol. 118, No. 1. pp. 71-84.
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abstract = "Incenp is an essential mitotic protein that, together with Aurora B, Survivin, and Borealin, forms the core of the chromosomal passenger protein complex (CPC). The CPC regulates various mitotic processes and functions to maintain genomic stability. The proper subcellular localization of the CPC and its full catalytic activity require the presence of each core subunit in the complex. We have investigated the mitotic tasks of the CPC using a function blocking antibody against Incenp microinjected into cells at different mitotic phases. This method allowed temporal analysis of CPC functions without perturbation of complex assembly or activity prior to injection. We have also studied the dynamic properties of Incenp and Aurora B using fusion protein photobleaching. We found that in early mitotic cells, Incenp and Aurora B exhibit dynamic turnover at centromeres, which is prevented by the anti-Incenp antibody. In these cells, the loss of centromeric CPC turnover is accompanied by forced mitotic exit without the execution of cytokinesis. Introduction of anti-Incenp antibody into early anaphase cells causes abnormalities in sister chromatid separation through defects in anaphase spindle functions. In summary, our data uncovers new mitotic roles for the CPC in anaphase and proposes that CPC turnover at centromeres modulates spindle assembly checkpoint signaling.",
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Ahonen, L, Kukkonen, A, Pouwels, J, Bolton, MA, Jingle, CD, Stukenberg, PT & Kallio, M 2009, 'Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres', Chromosoma, vol. 118, no. 1, pp. 71-84. https://doi.org/10.1007/s00412-008-0178-0

Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres. / Ahonen, Leena; Kukkonen, Anu; Pouwels, Jeroen; Bolton, Margaret A.; Jingle, Christopher D.; Stukenberg, P. Todd; Kallio, Marko (Corresponding Author).

In: Chromosoma, Vol. 118, No. 1, 2009, p. 71-84.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres

AU - Ahonen, Leena

AU - Kukkonen, Anu

AU - Pouwels, Jeroen

AU - Bolton, Margaret A.

AU - Jingle, Christopher D.

AU - Stukenberg, P. Todd

AU - Kallio, Marko

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N2 - Incenp is an essential mitotic protein that, together with Aurora B, Survivin, and Borealin, forms the core of the chromosomal passenger protein complex (CPC). The CPC regulates various mitotic processes and functions to maintain genomic stability. The proper subcellular localization of the CPC and its full catalytic activity require the presence of each core subunit in the complex. We have investigated the mitotic tasks of the CPC using a function blocking antibody against Incenp microinjected into cells at different mitotic phases. This method allowed temporal analysis of CPC functions without perturbation of complex assembly or activity prior to injection. We have also studied the dynamic properties of Incenp and Aurora B using fusion protein photobleaching. We found that in early mitotic cells, Incenp and Aurora B exhibit dynamic turnover at centromeres, which is prevented by the anti-Incenp antibody. In these cells, the loss of centromeric CPC turnover is accompanied by forced mitotic exit without the execution of cytokinesis. Introduction of anti-Incenp antibody into early anaphase cells causes abnormalities in sister chromatid separation through defects in anaphase spindle functions. In summary, our data uncovers new mitotic roles for the CPC in anaphase and proposes that CPC turnover at centromeres modulates spindle assembly checkpoint signaling.

AB - Incenp is an essential mitotic protein that, together with Aurora B, Survivin, and Borealin, forms the core of the chromosomal passenger protein complex (CPC). The CPC regulates various mitotic processes and functions to maintain genomic stability. The proper subcellular localization of the CPC and its full catalytic activity require the presence of each core subunit in the complex. We have investigated the mitotic tasks of the CPC using a function blocking antibody against Incenp microinjected into cells at different mitotic phases. This method allowed temporal analysis of CPC functions without perturbation of complex assembly or activity prior to injection. We have also studied the dynamic properties of Incenp and Aurora B using fusion protein photobleaching. We found that in early mitotic cells, Incenp and Aurora B exhibit dynamic turnover at centromeres, which is prevented by the anti-Incenp antibody. In these cells, the loss of centromeric CPC turnover is accompanied by forced mitotic exit without the execution of cytokinesis. Introduction of anti-Incenp antibody into early anaphase cells causes abnormalities in sister chromatid separation through defects in anaphase spindle functions. In summary, our data uncovers new mitotic roles for the CPC in anaphase and proposes that CPC turnover at centromeres modulates spindle assembly checkpoint signaling.

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KW - mitotic proteins

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KW - anaphase

KW - genomic instability

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VL - 118

SP - 71

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JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

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