Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers

Pezhman Mohammadi (Corresponding Author), Aino Sesilja Aranko, Laura Lemetti, Zoran Cenev, Quan Zhou, Salla Virtanen, Christopher Paul Landowski, Merja Penttilä, Wolfgang J. Fischer, Wolfgang Wagermaier, Markus B. Linder (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

A central concept in molecular bioscience is how structure formation at different length scales is achieved. Here we use spider silk protein as a model to design new recombinant proteins that assemble into fibers. We made proteins with a three-block architecture with folded globular domains at each terminus of a truncated repetitive silk sequence. Aqueous solutions of these engineered proteins undergo liquid–liquid phase separation as an essential pre-assembly step before fibers can form by drawing in air. We show that two different forms of phase separation occur depending on solution conditions, but only one form leads to fiber assembly. Structural variants with one-block or two-block architectures do not lead to fibers. Fibers show strong adhesion to surfaces and self-fusing properties when placed into contact with each other. Our results show a link between protein architecture and phase separation behavior suggesting a general approach for understanding protein assembly from dilute solutions into functional structures
Original languageEnglish
Article number86
Number of pages12
JournalCommunications biology
Volume1
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

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Phase transitions
Fibers
Phase separation
Silk
Proteins
Recombinant Proteins
Adhesion
Air

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Mohammadi, Pezhman ; Aranko, Aino Sesilja ; Lemetti, Laura ; Cenev, Zoran ; Zhou, Quan ; Virtanen, Salla ; Landowski, Christopher Paul ; Penttilä, Merja ; Fischer, Wolfgang J. ; Wagermaier, Wolfgang ; Linder, Markus B. / Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers. In: Communications biology. 2018 ; Vol. 1.
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abstract = "A central concept in molecular bioscience is how structure formation at different length scales is achieved. Here we use spider silk protein as a model to design new recombinant proteins that assemble into fibers. We made proteins with a three-block architecture with folded globular domains at each terminus of a truncated repetitive silk sequence. Aqueous solutions of these engineered proteins undergo liquid–liquid phase separation as an essential pre-assembly step before fibers can form by drawing in air. We show that two different forms of phase separation occur depending on solution conditions, but only one form leads to fiber assembly. Structural variants with one-block or two-block architectures do not lead to fibers. Fibers show strong adhesion to surfaces and self-fusing properties when placed into contact with each other. Our results show a link between protein architecture and phase separation behavior suggesting a general approach for understanding protein assembly from dilute solutions into functional structures",
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Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers. / Mohammadi, Pezhman (Corresponding Author); Aranko, Aino Sesilja; Lemetti, Laura; Cenev, Zoran; Zhou, Quan; Virtanen, Salla; Landowski, Christopher Paul; Penttilä, Merja; Fischer, Wolfgang J.; Wagermaier, Wolfgang ; Linder, Markus B. (Corresponding Author).

In: Communications biology, Vol. 1, 86, 2018.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Mohammadi, Pezhman

AU - Aranko, Aino Sesilja

AU - Lemetti, Laura

AU - Cenev, Zoran

AU - Zhou, Quan

AU - Virtanen, Salla

AU - Landowski, Christopher Paul

AU - Penttilä, Merja

AU - Fischer, Wolfgang J.

AU - Wagermaier, Wolfgang

AU - Linder, Markus B.

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