Phenotypic and molecular assessment of antimicrobial resistance in Lactobacillus paracasei strains of food origin

Geert Huys (Corresponding Author), Klaas D'Haene, Morten Danielsen, Jaana Mättö, Maria Egervärn, Peter Vandamme

Research output: Contribution to journalArticleScientificpeer-review

34 Citations (Scopus)

Abstract

Antimicrobial resistance data in food-associated lactic acid bacteria (LAB) such as lactobacilli are mostly based on nonstandardized methodologies and/or have been obtained for only a limited number of strains. This susceptibility study included a diverse collection of 115 isolates mainly of food origin originally identified as Lactobacillus paracasei or Lactobacillus casei. Upon reidentification and removal of potential replicate isolates using repetitive DNA element PCR fingerprinting, 65 genotypically unique L. paracasei strains and the L. casei type strain were selected for broth microdilution and Etest assays using the LAB susceptibility test medium. In both methodologies, strains appeared uniformly susceptible to ampicillin and clindamycin but exhibited natural resistance to streptomycin and gentamicin. Three L. paracasei strains from cheese displayed acquired resistance to tetracycline (MIC ≥ 32 μg/ml) and/or to erythromycin (MIC >16 μ g/ml), which was linked to the presence of a tet(M) or tet(W) gene and/or an erm(B) gene, respectively. Partial sequencing revealed that the tet(M) genes found in two of these strains belonged to two tet(M) sequence homology groups previously found in enterococci. Collectively, phenotypic and genotypic data allowed us to propose tentative epidemiological cutoffs for L. paracasei and L. casei for differentiating susceptible strains from those strains harboring one or more acquired resistance factors.
Original languageEnglish
Pages (from-to)339-344
JournalJournal of Food Protection
Volume71
Issue number2
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

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Lactobacillus paracasei
Lactobacillus casei
antibiotic resistance
Food
Lactic Acid
Disk Diffusion Antimicrobial Tests
Genes
Bacteria
Tetracycline Resistance
Clindamycin
R Factors
Enterococcus
Cheese
Lactobacillus
Streptomycin
Erythromycin
Ampicillin
Sequence Homology
Gentamicins
Innate Immunity

Cite this

Huys, Geert ; D'Haene, Klaas ; Danielsen, Morten ; Mättö, Jaana ; Egervärn, Maria ; Vandamme, Peter. / Phenotypic and molecular assessment of antimicrobial resistance in Lactobacillus paracasei strains of food origin. In: Journal of Food Protection. 2008 ; Vol. 71, No. 2. pp. 339-344.
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abstract = "Antimicrobial resistance data in food-associated lactic acid bacteria (LAB) such as lactobacilli are mostly based on nonstandardized methodologies and/or have been obtained for only a limited number of strains. This susceptibility study included a diverse collection of 115 isolates mainly of food origin originally identified as Lactobacillus paracasei or Lactobacillus casei. Upon reidentification and removal of potential replicate isolates using repetitive DNA element PCR fingerprinting, 65 genotypically unique L. paracasei strains and the L. casei type strain were selected for broth microdilution and Etest assays using the LAB susceptibility test medium. In both methodologies, strains appeared uniformly susceptible to ampicillin and clindamycin but exhibited natural resistance to streptomycin and gentamicin. Three L. paracasei strains from cheese displayed acquired resistance to tetracycline (MIC ≥ 32 μg/ml) and/or to erythromycin (MIC >16 μ g/ml), which was linked to the presence of a tet(M) or tet(W) gene and/or an erm(B) gene, respectively. Partial sequencing revealed that the tet(M) genes found in two of these strains belonged to two tet(M) sequence homology groups previously found in enterococci. Collectively, phenotypic and genotypic data allowed us to propose tentative epidemiological cutoffs for L. paracasei and L. casei for differentiating susceptible strains from those strains harboring one or more acquired resistance factors.",
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Phenotypic and molecular assessment of antimicrobial resistance in Lactobacillus paracasei strains of food origin. / Huys, Geert (Corresponding Author); D'Haene, Klaas; Danielsen, Morten; Mättö, Jaana; Egervärn, Maria; Vandamme, Peter.

In: Journal of Food Protection, Vol. 71, No. 2, 2008, p. 339-344.

Research output: Contribution to journalArticleScientificpeer-review

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