Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

Budi J. Hidayat; Marilyn Wiebe; Niels T. Eriksen

doi:10.1016/j.enzmictec.2006.07.005

Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

Budi J. Hidayat, Marilyn Wiebe, Niels T. Eriksen (Corresponding Author)

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Abstract

In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.

Original language	English
Pages (from-to)	902-907
Journal	Enzyme and Microbial Technology
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/j.enzmictec.2006.07.005
Publication status	Published - 2007
MoE publication type	A1 Journal article-refereed

Keywords

Fusarium venenatum
Continuous flow culture
Acid phosphatase
Dilution rate
Phosphate concentration
pH

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10.1016/j.enzmictec.2006.07.005

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title = "Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase",

abstract = "In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.",

keywords = "Fusarium venenatum, Continuous flow culture, Acid phosphatase, Dilution rate, Phosphate concentration, pH",

author = "Hidayat, {Budi J.} and Marilyn Wiebe and Eriksen, {Niels T.}",

year = "2007",

doi = "10.1016/j.enzmictec.2006.07.005",

language = "English",

volume = "40",

pages = "902--907",

journal = "Enzyme and Microbial Technology",

issn = "0141-0229",

publisher = "Elsevier",

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T1 - Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

AU - Hidayat, Budi J.

AU - Wiebe, Marilyn

AU - Eriksen, Niels T.

PY - 2007

Y1 - 2007

N2 - In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.

AB - In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.

KW - Fusarium venenatum

KW - Continuous flow culture

KW - Acid phosphatase

KW - Dilution rate

KW - Phosphate concentration

KW - pH

U2 - 10.1016/j.enzmictec.2006.07.005

DO - 10.1016/j.enzmictec.2006.07.005

M3 - Article

VL - 40

SP - 902

EP - 907

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -