Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

Budi J. Hidayat, Marilyn Wiebe, Niels T. Eriksen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.
Original languageEnglish
Pages (from-to)902-907
JournalEnzyme and Microbial Technology
Volume40
Issue number4
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Phosphatases
Fusarium
Acid Phosphatase
Phosphates
Acids
Isoenzymes
Dilution
Productivity
Growth

Keywords

  • Fusarium venenatum
  • Continuous flow culture
  • Acid phosphatase
  • Dilution rate
  • Phosphate concentration
  • pH

Cite this

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title = "Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase",
abstract = "In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.",
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Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase. / Hidayat, Budi J.; Wiebe, Marilyn; Eriksen, Niels T. (Corresponding Author).

In: Enzyme and Microbial Technology, Vol. 40, No. 4, 2007, p. 902-907.

Research output: Contribution to journalArticleScientificpeer-review

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AB - In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.

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KW - Dilution rate

KW - Phosphate concentration

KW - pH

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