Abstract
In this paper we describe the production of acid phosphatase (ACP) by
Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at
different phosphate concentrations, specific growth rates, and pH values. ACP
production in F. venenatum was repressed by phosphate, and was only fully
de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP
was active from pH 2 to 7, and the activity could be attributed to one or
possibly two isozymes, the one showing a strong activity maximum at pH 6, and
the second showing a weaker activity maximum at pH 3. The specific ACP
productivity was highest at dilution rates below D = 0.06 h−1. ACP
production was also influenced by pH, which showed two, clearly separated
production maxima at culture pH 3.3 and 5.8.
Original language | English |
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Pages (from-to) | 902-907 |
Journal | Enzyme and Microbial Technology |
Volume | 40 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2007 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Fusarium venenatum
- Continuous flow culture
- Acid phosphatase
- Dilution rate
- Phosphate concentration
- pH