Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

Budi J. Hidayat, Marilyn Wiebe, Niels T. Eriksen (Corresponding Author)

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1 Citation (Scopus)

Abstract

In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.
Original languageEnglish
Pages (from-to)902-907
JournalEnzyme and Microbial Technology
Volume40
Issue number4
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • Fusarium venenatum
  • Continuous flow culture
  • Acid phosphatase
  • Dilution rate
  • Phosphate concentration
  • pH

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