Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 902-907 |
| Journal | Enzyme and Microbial Technology |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2007 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Fusarium venenatum
- Continuous flow culture
- Acid phosphatase
- Dilution rate
- Phosphate concentration
- pH
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Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase. / Hidayat, Budi J.; Wiebe, Marilyn; Eriksen, Niels T. (Corresponding Author).
In: Enzyme and Microbial Technology, Vol. 40, No. 4, 2007, p. 902-907.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase
AU - Hidayat, Budi J.
AU - Wiebe, Marilyn
AU - Eriksen, Niels T.
PY - 2007
Y1 - 2007
N2 - In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.
AB - In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.
KW - Fusarium venenatum
KW - Continuous flow culture
KW - Acid phosphatase
KW - Dilution rate
KW - Phosphate concentration
KW - pH
U2 - 10.1016/j.enzmictec.2006.07.005
DO - 10.1016/j.enzmictec.2006.07.005
M3 - Article
VL - 40
SP - 902
EP - 907
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 4
ER -