TY - CHAP
T1 - Physiological response of Saccharomyces cerevisiae to change in oxygen provision
AU - Rintala, Eija
AU - Toivari, Mervi
AU - Wiebe, Marilyn
AU - Tamminen, Anu
AU - Salusjärvi, Laura
AU - Huuskonen, Anne
AU - Simolin, Helena
AU - Kokkonen, J.
AU - Kiuru, J.
AU - Maaheimo, Hannu
AU - Ruohonen, Laura
AU - Penttilä, Merja
PY - 2006
Y1 - 2006
N2 - Oxygen is a major determinant of cellular physiology and
is of particular importance in industrial biotech
processes, in which the cost of oxygen supply must be
balanced with performance requirements. Bakers' yeast,
Saccharomyces cerevisiae, is one of the most exploited
industrial organisms and regardless of whether the
process is aerated, as in biomass and protein
manufacture, or non-aerated, as in brewing, the role of
oxygen is crucial.
Saccharomyces cerevisiae was grown in glucose-limited
chemostat culture with 0% (anaerobic), 0.5%, 1%, 2.8% or
21% oxygen (D = 0.10 h-1, pH 5). After steady states had
been achieved, the conditions were shifted from aerobic
to anaerobic or from anaerobic to aerobic. During and
following the shift in conditions, samples were removed
and analysed for changes at the metabolite and transcript
(primarily those involved in carbon metabolism) levels.
Metabolites of upper glycolysis generally increased,
following a transition to anaerobic conditions, while the
metabolites of lower glycolysis generally decreased.
Metabolites from the TCA cycle generally increased under
the same conditions. Similar, opposite responses were
observed when conditions were shifted from anaerobic to
aerobic. Gene regulation, however, was more complex, with
several genes showing transient up- or down-regulation
following a change in conditions. Responses to a shift
from aerobic to anaerobic conditions were generally
similar, regardless of the oxygen concentration prior to
the shift. Based on these results, biomarkers will be
identified which can be used in assessing oxygen
provision to S. cerevisiae cultures.
AB - Oxygen is a major determinant of cellular physiology and
is of particular importance in industrial biotech
processes, in which the cost of oxygen supply must be
balanced with performance requirements. Bakers' yeast,
Saccharomyces cerevisiae, is one of the most exploited
industrial organisms and regardless of whether the
process is aerated, as in biomass and protein
manufacture, or non-aerated, as in brewing, the role of
oxygen is crucial.
Saccharomyces cerevisiae was grown in glucose-limited
chemostat culture with 0% (anaerobic), 0.5%, 1%, 2.8% or
21% oxygen (D = 0.10 h-1, pH 5). After steady states had
been achieved, the conditions were shifted from aerobic
to anaerobic or from anaerobic to aerobic. During and
following the shift in conditions, samples were removed
and analysed for changes at the metabolite and transcript
(primarily those involved in carbon metabolism) levels.
Metabolites of upper glycolysis generally increased,
following a transition to anaerobic conditions, while the
metabolites of lower glycolysis generally decreased.
Metabolites from the TCA cycle generally increased under
the same conditions. Similar, opposite responses were
observed when conditions were shifted from anaerobic to
aerobic. Gene regulation, however, was more complex, with
several genes showing transient up- or down-regulation
following a change in conditions. Responses to a shift
from aerobic to anaerobic conditions were generally
similar, regardless of the oxygen concentration prior to
the shift. Based on these results, biomarkers will be
identified which can be used in assessing oxygen
provision to S. cerevisiae cultures.
M3 - Conference abstract in proceedings
SN - 951-38-6307-7
T3 - VTT Symposium
SP - 39
BT - International Specialised Symposium on Yeasts ISSY25
PB - VTT Technical Research Centre of Finland
CY - Espoo
T2 - International Specialised Symposium on Yeasts, ISSY 25
Y2 - 18 June 2006 through 21 June 2006
ER -