Physiological response of Saccharomyces cerevisiae to change in oxygen provision

Eija Rintala, Mervi Toivari, Marilyn Wiebe, Anu Tamminen, Laura Salusjärvi, Anne Huuskonen, Helena Simolin, J. Kokkonen, J. Kiuru, Hannu Maaheimo, Laura Ruohonen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Oxygen is a major determinant of cellular physiology and is of particular importance in industrial biotech processes, in which the cost of oxygen supply must be balanced with performance requirements. Bakers' yeast, Saccharomyces cerevisiae, is one of the most exploited industrial organisms and regardless of whether the process is aerated, as in biomass and protein manufacture, or non-aerated, as in brewing, the role of oxygen is crucial. Saccharomyces cerevisiae was grown in glucose-limited chemostat culture with 0% (anaerobic), 0.5%, 1%, 2.8% or 21% oxygen (D = 0.10 h-1, pH 5). After steady states had been achieved, the conditions were shifted from aerobic to anaerobic or from anaerobic to aerobic. During and following the shift in conditions, samples were removed and analysed for changes at the metabolite and transcript (primarily those involved in carbon metabolism) levels. Metabolites of upper glycolysis generally increased, following a transition to anaerobic conditions, while the metabolites of lower glycolysis generally decreased. Metabolites from the TCA cycle generally increased under the same conditions. Similar, opposite responses were observed when conditions were shifted from anaerobic to aerobic. Gene regulation, however, was more complex, with several genes showing transient up- or down-regulation following a change in conditions. Responses to a shift from aerobic to anaerobic conditions were generally similar, regardless of the oxygen concentration prior to the shift. Based on these results, biomarkers will be identified which can be used in assessing oxygen provision to S. cerevisiae cultures.
Original languageEnglish
Title of host publicationInternational Specialised Symposium on Yeasts ISSY25
Subtitle of host publicationSystems Biology of Yeasts - from Models to Applications
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages39
ISBN (Electronic)951-38-6308-5
ISBN (Print)951-38-6307-7
Publication statusPublished - 2006
EventInternational Specialised Symposium on Yeasts, ISSY 25 - Espoo, Finland
Duration: 18 Jun 200621 Jun 2006

Publication series

SeriesVTT Symposium
Number242
ISSN0357-9387

Conference

ConferenceInternational Specialised Symposium on Yeasts, ISSY 25
Abbreviated titleISSY 25
CountryFinland
CityEspoo
Period18/06/0621/06/06

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physiological response
oxygen
metabolite
anoxic conditions
chemostat
gene
yeast
physiology
biomarker
glucose
metabolism
protein
carbon
biomass
cost
regulation

Cite this

Rintala, E., Toivari, M., Wiebe, M., Tamminen, A., Salusjärvi, L., Huuskonen, A., ... Penttilä, M. (2006). Physiological response of Saccharomyces cerevisiae to change in oxygen provision. In International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications (pp. 39). [T24] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 242
Rintala, Eija ; Toivari, Mervi ; Wiebe, Marilyn ; Tamminen, Anu ; Salusjärvi, Laura ; Huuskonen, Anne ; Simolin, Helena ; Kokkonen, J. ; Kiuru, J. ; Maaheimo, Hannu ; Ruohonen, Laura ; Penttilä, Merja. / Physiological response of Saccharomyces cerevisiae to change in oxygen provision. International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo : VTT Technical Research Centre of Finland, 2006. pp. 39 (VTT Symposium; No. 242).
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Rintala, E, Toivari, M, Wiebe, M, Tamminen, A, Salusjärvi, L, Huuskonen, A, Simolin, H, Kokkonen, J, Kiuru, J, Maaheimo, H, Ruohonen, L & Penttilä, M 2006, Physiological response of Saccharomyces cerevisiae to change in oxygen provision. in International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications., T24, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 242, pp. 39, International Specialised Symposium on Yeasts, ISSY 25 , Espoo, Finland, 18/06/06.

Physiological response of Saccharomyces cerevisiae to change in oxygen provision. / Rintala, Eija; Toivari, Mervi; Wiebe, Marilyn; Tamminen, Anu; Salusjärvi, Laura; Huuskonen, Anne; Simolin, Helena; Kokkonen, J.; Kiuru, J.; Maaheimo, Hannu; Ruohonen, Laura; Penttilä, Merja.

International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo : VTT Technical Research Centre of Finland, 2006. p. 39 T24 (VTT Symposium; No. 242).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Physiological response of Saccharomyces cerevisiae to change in oxygen provision

AU - Rintala, Eija

AU - Toivari, Mervi

AU - Wiebe, Marilyn

AU - Tamminen, Anu

AU - Salusjärvi, Laura

AU - Huuskonen, Anne

AU - Simolin, Helena

AU - Kokkonen, J.

AU - Kiuru, J.

AU - Maaheimo, Hannu

AU - Ruohonen, Laura

AU - Penttilä, Merja

PY - 2006

Y1 - 2006

N2 - Oxygen is a major determinant of cellular physiology and is of particular importance in industrial biotech processes, in which the cost of oxygen supply must be balanced with performance requirements. Bakers' yeast, Saccharomyces cerevisiae, is one of the most exploited industrial organisms and regardless of whether the process is aerated, as in biomass and protein manufacture, or non-aerated, as in brewing, the role of oxygen is crucial. Saccharomyces cerevisiae was grown in glucose-limited chemostat culture with 0% (anaerobic), 0.5%, 1%, 2.8% or 21% oxygen (D = 0.10 h-1, pH 5). After steady states had been achieved, the conditions were shifted from aerobic to anaerobic or from anaerobic to aerobic. During and following the shift in conditions, samples were removed and analysed for changes at the metabolite and transcript (primarily those involved in carbon metabolism) levels. Metabolites of upper glycolysis generally increased, following a transition to anaerobic conditions, while the metabolites of lower glycolysis generally decreased. Metabolites from the TCA cycle generally increased under the same conditions. Similar, opposite responses were observed when conditions were shifted from anaerobic to aerobic. Gene regulation, however, was more complex, with several genes showing transient up- or down-regulation following a change in conditions. Responses to a shift from aerobic to anaerobic conditions were generally similar, regardless of the oxygen concentration prior to the shift. Based on these results, biomarkers will be identified which can be used in assessing oxygen provision to S. cerevisiae cultures.

AB - Oxygen is a major determinant of cellular physiology and is of particular importance in industrial biotech processes, in which the cost of oxygen supply must be balanced with performance requirements. Bakers' yeast, Saccharomyces cerevisiae, is one of the most exploited industrial organisms and regardless of whether the process is aerated, as in biomass and protein manufacture, or non-aerated, as in brewing, the role of oxygen is crucial. Saccharomyces cerevisiae was grown in glucose-limited chemostat culture with 0% (anaerobic), 0.5%, 1%, 2.8% or 21% oxygen (D = 0.10 h-1, pH 5). After steady states had been achieved, the conditions were shifted from aerobic to anaerobic or from anaerobic to aerobic. During and following the shift in conditions, samples were removed and analysed for changes at the metabolite and transcript (primarily those involved in carbon metabolism) levels. Metabolites of upper glycolysis generally increased, following a transition to anaerobic conditions, while the metabolites of lower glycolysis generally decreased. Metabolites from the TCA cycle generally increased under the same conditions. Similar, opposite responses were observed when conditions were shifted from anaerobic to aerobic. Gene regulation, however, was more complex, with several genes showing transient up- or down-regulation following a change in conditions. Responses to a shift from aerobic to anaerobic conditions were generally similar, regardless of the oxygen concentration prior to the shift. Based on these results, biomarkers will be identified which can be used in assessing oxygen provision to S. cerevisiae cultures.

M3 - Conference abstract in proceedings

SN - 951-38-6307-7

T3 - VTT Symposium

SP - 39

BT - International Specialised Symposium on Yeasts ISSY25

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Rintala E, Toivari M, Wiebe M, Tamminen A, Salusjärvi L, Huuskonen A et al. Physiological response of Saccharomyces cerevisiae to change in oxygen provision. In International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo: VTT Technical Research Centre of Finland. 2006. p. 39. T24. (VTT Symposium; No. 242).