Phytic Acid Reduction by Bioprocessing as a Tool to Improve the in Vitro Digestibility of Faba Bean Protein

Natalia Rosa-Sibakov (Corresponding Author), Margherita Re, Anni Karsma, Arja Laitila, Emilia Nordlund

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

In legumes such as faba bean, phytic acid can form very stable complexes with proteins, thus hindering their accessibility for gastrointestinal digestion. This study was set up to investigate the influence of enzymatic phytase treatment and lactic acid bacteria fermentation (Lactobacillus plantarum VTT E-78076) on phytic acid reduction as well as on the protein quality and digestibility of faba bean flour. The soluble protein and release of free amino acids from faba bean flours were followed during a simulated in vitro digestion process. Phytase treatment efficiently reduced the amount of phytic acid in faba bean flour up to 89% degradation. The reduction of phytic acid content caused a shift in the protein solubility curve, showing higher solubility levels at low pH. Further, the enzyme-aided degradation of phytic acid considerably enhanced the digestibility of faba bean proteins (dose-dependent) and the release of free amino nitrogen in the first stage of digestion (gastric phase). The results propose the benefits of phytase treatment in plant-based foods where the phytic acid content may hinder the digestion of protein.

Original languageEnglish
Pages (from-to)10394-10399
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number40
DOIs
Publication statusPublished - 10 Oct 2018
MoE publication typeNot Eligible

Fingerprint

bioprocessing
Vicia faba
Phytic Acid
in vitro digestibility
faba beans
phytic acid
6-Phytase
phytases
Flour
Digestion
Proteins
proteins
digestion
Solubility
digestibility
plant-based foods
Lactobacillus plantarum
Degradation
Edible Plants
amino nitrogen

Keywords

  • faba bean
  • lactic acid bacteria fermentation
  • phytase
  • protein digestibility

Cite this

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title = "Phytic Acid Reduction by Bioprocessing as a Tool to Improve the in Vitro Digestibility of Faba Bean Protein",
abstract = "In legumes such as faba bean, phytic acid can form very stable complexes with proteins, thus hindering their accessibility for gastrointestinal digestion. This study was set up to investigate the influence of enzymatic phytase treatment and lactic acid bacteria fermentation (Lactobacillus plantarum VTT E-78076) on phytic acid reduction as well as on the protein quality and digestibility of faba bean flour. The soluble protein and release of free amino acids from faba bean flours were followed during a simulated in vitro digestion process. Phytase treatment efficiently reduced the amount of phytic acid in faba bean flour up to 89{\%} degradation. The reduction of phytic acid content caused a shift in the protein solubility curve, showing higher solubility levels at low pH. Further, the enzyme-aided degradation of phytic acid considerably enhanced the digestibility of faba bean proteins (dose-dependent) and the release of free amino nitrogen in the first stage of digestion (gastric phase). The results propose the benefits of phytase treatment in plant-based foods where the phytic acid content may hinder the digestion of protein.",
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Phytic Acid Reduction by Bioprocessing as a Tool to Improve the in Vitro Digestibility of Faba Bean Protein. / Rosa-Sibakov, Natalia (Corresponding Author); Re, Margherita; Karsma, Anni; Laitila, Arja; Nordlund, Emilia.

In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 40, 10.10.2018, p. 10394-10399.

Research output: Contribution to journalArticleScientificpeer-review

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