Pilot scale production of a Trichoderma reesei endo-β-glucanase by brewer's yeast

Beat D. Zurbriggen, Merja E. Penttilä, Liisa Viikari, Michael J. Bailey

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16 Citations (Scopus)

Abstract

Endo-β-glucanase I (EGI) of Trichoderma reesei was produced in laboratory and pilot scale using recombinant strains of "bottom-fermenting" Saccharomyces cerevisiae. The gene egl1 was integrated in the chromosome or an expression cassette was inserted on a multicopy plasmid. Expression levels were compared in a laboratory scale bioreactor. The best EGI-producing strain was cultivated in pilot scale. Adsorbent treatment was used to remove endogenous yeast proteins and other impurities from the culture filtrate during concentration. Effective pilot scale one-step purification of the EGI protein was obtained using DEAE-Sepharose, on which EGI was weakly bound. The purified enzyme reacted with antibodies prepared against T. reesei EGI and catalyzed the hydrolysis of both insoluble and soluble substrates.

Original languageEnglish
Pages (from-to)133-146
JournalJournal of Biotechnology
Volume17
Issue number2
DOIs
Publication statusPublished - 1 Jan 1991
MoE publication typeA1 Journal article-refereed

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Keywords

  • Downstream processing
  • Hydrolysis testing
  • Pilot scale production
  • Saccharomyces cerevisiae
  • Trichoderma reesei endo-β-glucanase I

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