Plant defence compounds inhibit the growth of human pathogenic bacteria

Research output: Contribution to conferenceConference articleScientific

Abstract

Berries are good sources of phenolic compounds, which often act as defence compounds in plants. In humans plant phenolics besides their other health-inducing effects have been found to possess antimicrobial activities. Our previous studies showed that phenolic berry extracts especially inhibited the growth of Gram-negative but not Gram-positive bacteria such as Lactobacillus. Especially cloudberry, raspberry and strawberry extracts proved to be strong inhibitors of the avirulent Salmonella (1). Materials and Methods Bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), raspberry (Rubus idaeus, var. Ottawa), cloudberry (Rubus chamaemorus), strawberry (Fragaria ananassa Senga Sengana), blackcurrant (Ribes nigrum, var. Öjeby) and sea buckthorn berry (Hippophae rhamnoides), were used in the study. For antimicrobial studies berries were freeze-dried and ground to fine powder. Phenolics were extracted from the berries by aqueous 70% acetone (2). Antimicrobial activity of freeze-dried grounded berries (2 or 10 mg/ml) and phenolic berry extracts (1 or 5 mg/ml) was analysed by determining the bacterial growth curves in liquid cultures (1). The bacterial strains used were Listeria monocytogenes VTT E-991205, L. innocua VTT E-981011, Salmonella enterica ser. Typhimurium VTT E-981151, S. enterica ser. Infantis VTT E-97738, Staphylococcus aureus VTT E-70045 and Lactobacillus rhamnosus VTT E-96666 (ATCC 53103). Results Salmonella strains were inhibited by all the berries. Cloudberry, raspberry, strawberry and bilberry extracts inhibited the growth during the first 12 h incubation. However, during the next 12 h the number of viable cells started to increase. Cloudberry extract was the best inhibitor showing strong antimicrobial effects. Staphylococcus strains were strongly inhibited by all the berries and berry extracts. The number of viable cells did not increase at the end of cultivation. The tested Listeria or Lactobacillus strains were not sensitive to berries or berry extracts, with the exception of cranberry, which showed inhibitory effects against Listeria bacteria. Conclusions The results showed that antimicrobial properties of the berry compounds aren't restricted to the avirulent Salmonella but inhibited also the virulent strains. The inhibition of the Staphylococcus aureus suggested that also the structure of Gram-positive bacteria is sensitive to the antimicrobial compounds of the berries. Bioactive berry compounds seem to inhibit the growth of pathogenic bacteria with several mechanisms. The difference in the antimicrobial properties of the berries and the berry extracts also suggested that other bioactive compounds are involved in the cell growth inhibition. In the future, the most interesting berry extracts will be fractionated with the aim of identifying the antimicrobially active components. In addition the mechanism of action against gastrointestinal bacteria, especially intestinal pathogens, will be extensively studied. The results will be utilised in functional food development and in pharmaceutical applications.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
Event2nd EPSO Conference on Interactions in Plant Biology: Cells, plants and communities - Ischia, Italy
Duration: 10 Oct 200414 Oct 2004
Conference number: 2

Conference

Conference2nd EPSO Conference on Interactions in Plant Biology
CountryItaly
CityIschia
Period10/10/0414/10/04

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human growth
small fruits
bacteria
Rubus chamaemorus
extracts
raspberries
anti-infective properties
anti-infective agents
strawberries
Vaccinium vitis-idaea
bilberries
Hippophae rhamnoides
Salmonella
cranberries
Vaccinium
Listeria
Gram-positive bacteria
Lactobacillus
Staphylococcus aureus
Ribes nigrum

Cite this

Puupponen-Pimiä, R., Nohynek, L., Kähkönen, M., Heinonen, M., & Oksman-Caldentey, K-M. (2004). Plant defence compounds inhibit the growth of human pathogenic bacteria. Paper presented at 2nd EPSO Conference on Interactions in Plant Biology, Ischia, Italy.
Puupponen-Pimiä, Riitta ; Nohynek, Liisa ; Kähkönen, M. ; Heinonen, M. ; Oksman-Caldentey, Kirsi-Marja. / Plant defence compounds inhibit the growth of human pathogenic bacteria. Paper presented at 2nd EPSO Conference on Interactions in Plant Biology, Ischia, Italy.
@conference{49451ac56d8a4e259602ea18dbc1e15e,
title = "Plant defence compounds inhibit the growth of human pathogenic bacteria",
abstract = "Berries are good sources of phenolic compounds, which often act as defence compounds in plants. In humans plant phenolics besides their other health-inducing effects have been found to possess antimicrobial activities. Our previous studies showed that phenolic berry extracts especially inhibited the growth of Gram-negative but not Gram-positive bacteria such as Lactobacillus. Especially cloudberry, raspberry and strawberry extracts proved to be strong inhibitors of the avirulent Salmonella (1). Materials and Methods Bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), raspberry (Rubus idaeus, var. Ottawa), cloudberry (Rubus chamaemorus), strawberry (Fragaria ananassa Senga Sengana), blackcurrant (Ribes nigrum, var. {\"O}jeby) and sea buckthorn berry (Hippophae rhamnoides), were used in the study. For antimicrobial studies berries were freeze-dried and ground to fine powder. Phenolics were extracted from the berries by aqueous 70{\%} acetone (2). Antimicrobial activity of freeze-dried grounded berries (2 or 10 mg/ml) and phenolic berry extracts (1 or 5 mg/ml) was analysed by determining the bacterial growth curves in liquid cultures (1). The bacterial strains used were Listeria monocytogenes VTT E-991205, L. innocua VTT E-981011, Salmonella enterica ser. Typhimurium VTT E-981151, S. enterica ser. Infantis VTT E-97738, Staphylococcus aureus VTT E-70045 and Lactobacillus rhamnosus VTT E-96666 (ATCC 53103). Results Salmonella strains were inhibited by all the berries. Cloudberry, raspberry, strawberry and bilberry extracts inhibited the growth during the first 12 h incubation. However, during the next 12 h the number of viable cells started to increase. Cloudberry extract was the best inhibitor showing strong antimicrobial effects. Staphylococcus strains were strongly inhibited by all the berries and berry extracts. The number of viable cells did not increase at the end of cultivation. The tested Listeria or Lactobacillus strains were not sensitive to berries or berry extracts, with the exception of cranberry, which showed inhibitory effects against Listeria bacteria. Conclusions The results showed that antimicrobial properties of the berry compounds aren't restricted to the avirulent Salmonella but inhibited also the virulent strains. The inhibition of the Staphylococcus aureus suggested that also the structure of Gram-positive bacteria is sensitive to the antimicrobial compounds of the berries. Bioactive berry compounds seem to inhibit the growth of pathogenic bacteria with several mechanisms. The difference in the antimicrobial properties of the berries and the berry extracts also suggested that other bioactive compounds are involved in the cell growth inhibition. In the future, the most interesting berry extracts will be fractionated with the aim of identifying the antimicrobially active components. In addition the mechanism of action against gastrointestinal bacteria, especially intestinal pathogens, will be extensively studied. The results will be utilised in functional food development and in pharmaceutical applications.",
author = "Riitta Puupponen-Pimi{\"a} and Liisa Nohynek and M. K{\"a}hk{\"o}nen and M. Heinonen and Kirsi-Marja Oksman-Caldentey",
year = "2004",
language = "English",
note = "2nd EPSO Conference on Interactions in Plant Biology : Cells, plants and communities ; Conference date: 10-10-2004 Through 14-10-2004",

}

Puupponen-Pimiä, R, Nohynek, L, Kähkönen, M, Heinonen, M & Oksman-Caldentey, K-M 2004, 'Plant defence compounds inhibit the growth of human pathogenic bacteria' Paper presented at 2nd EPSO Conference on Interactions in Plant Biology, Ischia, Italy, 10/10/04 - 14/10/04, .

Plant defence compounds inhibit the growth of human pathogenic bacteria. / Puupponen-Pimiä, Riitta; Nohynek, Liisa; Kähkönen, M.; Heinonen, M.; Oksman-Caldentey, Kirsi-Marja.

2004. Paper presented at 2nd EPSO Conference on Interactions in Plant Biology, Ischia, Italy.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Plant defence compounds inhibit the growth of human pathogenic bacteria

AU - Puupponen-Pimiä, Riitta

AU - Nohynek, Liisa

AU - Kähkönen, M.

AU - Heinonen, M.

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2004

Y1 - 2004

N2 - Berries are good sources of phenolic compounds, which often act as defence compounds in plants. In humans plant phenolics besides their other health-inducing effects have been found to possess antimicrobial activities. Our previous studies showed that phenolic berry extracts especially inhibited the growth of Gram-negative but not Gram-positive bacteria such as Lactobacillus. Especially cloudberry, raspberry and strawberry extracts proved to be strong inhibitors of the avirulent Salmonella (1). Materials and Methods Bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), raspberry (Rubus idaeus, var. Ottawa), cloudberry (Rubus chamaemorus), strawberry (Fragaria ananassa Senga Sengana), blackcurrant (Ribes nigrum, var. Öjeby) and sea buckthorn berry (Hippophae rhamnoides), were used in the study. For antimicrobial studies berries were freeze-dried and ground to fine powder. Phenolics were extracted from the berries by aqueous 70% acetone (2). Antimicrobial activity of freeze-dried grounded berries (2 or 10 mg/ml) and phenolic berry extracts (1 or 5 mg/ml) was analysed by determining the bacterial growth curves in liquid cultures (1). The bacterial strains used were Listeria monocytogenes VTT E-991205, L. innocua VTT E-981011, Salmonella enterica ser. Typhimurium VTT E-981151, S. enterica ser. Infantis VTT E-97738, Staphylococcus aureus VTT E-70045 and Lactobacillus rhamnosus VTT E-96666 (ATCC 53103). Results Salmonella strains were inhibited by all the berries. Cloudberry, raspberry, strawberry and bilberry extracts inhibited the growth during the first 12 h incubation. However, during the next 12 h the number of viable cells started to increase. Cloudberry extract was the best inhibitor showing strong antimicrobial effects. Staphylococcus strains were strongly inhibited by all the berries and berry extracts. The number of viable cells did not increase at the end of cultivation. The tested Listeria or Lactobacillus strains were not sensitive to berries or berry extracts, with the exception of cranberry, which showed inhibitory effects against Listeria bacteria. Conclusions The results showed that antimicrobial properties of the berry compounds aren't restricted to the avirulent Salmonella but inhibited also the virulent strains. The inhibition of the Staphylococcus aureus suggested that also the structure of Gram-positive bacteria is sensitive to the antimicrobial compounds of the berries. Bioactive berry compounds seem to inhibit the growth of pathogenic bacteria with several mechanisms. The difference in the antimicrobial properties of the berries and the berry extracts also suggested that other bioactive compounds are involved in the cell growth inhibition. In the future, the most interesting berry extracts will be fractionated with the aim of identifying the antimicrobially active components. In addition the mechanism of action against gastrointestinal bacteria, especially intestinal pathogens, will be extensively studied. The results will be utilised in functional food development and in pharmaceutical applications.

AB - Berries are good sources of phenolic compounds, which often act as defence compounds in plants. In humans plant phenolics besides their other health-inducing effects have been found to possess antimicrobial activities. Our previous studies showed that phenolic berry extracts especially inhibited the growth of Gram-negative but not Gram-positive bacteria such as Lactobacillus. Especially cloudberry, raspberry and strawberry extracts proved to be strong inhibitors of the avirulent Salmonella (1). Materials and Methods Bilberry (Vaccinium myrtillus), lingonberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), raspberry (Rubus idaeus, var. Ottawa), cloudberry (Rubus chamaemorus), strawberry (Fragaria ananassa Senga Sengana), blackcurrant (Ribes nigrum, var. Öjeby) and sea buckthorn berry (Hippophae rhamnoides), were used in the study. For antimicrobial studies berries were freeze-dried and ground to fine powder. Phenolics were extracted from the berries by aqueous 70% acetone (2). Antimicrobial activity of freeze-dried grounded berries (2 or 10 mg/ml) and phenolic berry extracts (1 or 5 mg/ml) was analysed by determining the bacterial growth curves in liquid cultures (1). The bacterial strains used were Listeria monocytogenes VTT E-991205, L. innocua VTT E-981011, Salmonella enterica ser. Typhimurium VTT E-981151, S. enterica ser. Infantis VTT E-97738, Staphylococcus aureus VTT E-70045 and Lactobacillus rhamnosus VTT E-96666 (ATCC 53103). Results Salmonella strains were inhibited by all the berries. Cloudberry, raspberry, strawberry and bilberry extracts inhibited the growth during the first 12 h incubation. However, during the next 12 h the number of viable cells started to increase. Cloudberry extract was the best inhibitor showing strong antimicrobial effects. Staphylococcus strains were strongly inhibited by all the berries and berry extracts. The number of viable cells did not increase at the end of cultivation. The tested Listeria or Lactobacillus strains were not sensitive to berries or berry extracts, with the exception of cranberry, which showed inhibitory effects against Listeria bacteria. Conclusions The results showed that antimicrobial properties of the berry compounds aren't restricted to the avirulent Salmonella but inhibited also the virulent strains. The inhibition of the Staphylococcus aureus suggested that also the structure of Gram-positive bacteria is sensitive to the antimicrobial compounds of the berries. Bioactive berry compounds seem to inhibit the growth of pathogenic bacteria with several mechanisms. The difference in the antimicrobial properties of the berries and the berry extracts also suggested that other bioactive compounds are involved in the cell growth inhibition. In the future, the most interesting berry extracts will be fractionated with the aim of identifying the antimicrobially active components. In addition the mechanism of action against gastrointestinal bacteria, especially intestinal pathogens, will be extensively studied. The results will be utilised in functional food development and in pharmaceutical applications.

M3 - Conference article

ER -

Puupponen-Pimiä R, Nohynek L, Kähkönen M, Heinonen M, Oksman-Caldentey K-M. Plant defence compounds inhibit the growth of human pathogenic bacteria. 2004. Paper presented at 2nd EPSO Conference on Interactions in Plant Biology, Ischia, Italy.