Plant-made nervous necrosis virus-like particles protect fish against disease

Johanna Marsian, Daniel L. Hurdiss, Neil A. Ranson, Anneli Ritala, Richard Paley, Irene Cano, George P. Lomonossoff

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

Original languageEnglish
Article number880
JournalFrontiers in Plant Science
Volume10
DOIs
Publication statusPublished - 9 Jul 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

fish diseases
virus-like particles
necrosis
vertebrate viruses
viruses
Nicotiana benthamiana
Dicentrarchus labrax
Gadus morhua
bass
coat proteins
aquaculture
tobacco
genetically modified organisms
vaccines
fish
proteins
cells

Keywords

  • Atlantic cod nervous necrosis virus
  • Protective immunity
  • Sea bass
  • Transient expression
  • Virus-like particles

Cite this

Marsian, J., Hurdiss, D. L., Ranson, N. A., Ritala, A., Paley, R., Cano, I., & Lomonossoff, G. P. (2019). Plant-made nervous necrosis virus-like particles protect fish against disease. Frontiers in Plant Science, 10, [880]. https://doi.org/10.3389/fpls.2019.00880
Marsian, Johanna ; Hurdiss, Daniel L. ; Ranson, Neil A. ; Ritala, Anneli ; Paley, Richard ; Cano, Irene ; Lomonossoff, George P. / Plant-made nervous necrosis virus-like particles protect fish against disease. In: Frontiers in Plant Science. 2019 ; Vol. 10.
@article{d960373c39424aa08491b9dc167ce39e,
title = "Plant-made nervous necrosis virus-like particles protect fish against disease",
abstract = "Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.",
keywords = "Atlantic cod nervous necrosis virus, Protective immunity, Sea bass, Transient expression, Virus-like particles",
author = "Johanna Marsian and Hurdiss, {Daniel L.} and Ranson, {Neil A.} and Anneli Ritala and Richard Paley and Irene Cano and Lomonossoff, {George P.}",
year = "2019",
month = "7",
day = "9",
doi = "10.3389/fpls.2019.00880",
language = "English",
volume = "10",
journal = "Frontiers in Plant Science",
issn = "1664-462X",

}

Plant-made nervous necrosis virus-like particles protect fish against disease. / Marsian, Johanna; Hurdiss, Daniel L.; Ranson, Neil A.; Ritala, Anneli; Paley, Richard; Cano, Irene; Lomonossoff, George P.

In: Frontiers in Plant Science, Vol. 10, 880, 09.07.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Plant-made nervous necrosis virus-like particles protect fish against disease

AU - Marsian, Johanna

AU - Hurdiss, Daniel L.

AU - Ranson, Neil A.

AU - Ritala, Anneli

AU - Paley, Richard

AU - Cano, Irene

AU - Lomonossoff, George P.

PY - 2019/7/9

Y1 - 2019/7/9

N2 - Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

AB - Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

KW - Atlantic cod nervous necrosis virus

KW - Protective immunity

KW - Sea bass

KW - Transient expression

KW - Virus-like particles

UR - http://www.scopus.com/inward/record.url?scp=85069469768&partnerID=8YFLogxK

U2 - 10.3389/fpls.2019.00880

DO - 10.3389/fpls.2019.00880

M3 - Article

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 880

ER -