Plant-made nervous necrosis virus-like particles protect fish against disease

Johanna Marsian; Daniel L. Hurdiss; Neil A. Ranson; Anneli Ritala; Richard Paley; Irene Cano; George P. Lomonossoff

doi:10.3389/fpls.2019.00880

Plant-made nervous necrosis virus-like particles protect fish against disease

Johanna Marsian
, Daniel L. Hurdiss
, Neil A. Ranson
, Anneli Ritala
, Richard Paley
, Irene Cano
, George P. Lomonossoff^*

^*Corresponding author for this work

John Innes Centre
University of Leeds
Centre for the Environment Fisheries and Aquaculture Science

Research output: Contribution to journal › Article › Scientific › peer-review

44 Citations (Scopus)

Abstract

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

Original language	English
Article number	880
Journal	Frontiers in Plant Science
Volume	10
DOIs	https://doi.org/10.3389/fpls.2019.00880
Publication status	Published - 9 Jul 2019
MoE publication type	A1 Journal article-refereed

Funding

At the John Innes Centre (JIC) this work was supported by the UK Biotechnological and Biological Sciences Research Council (BBSRC) grant BB/M027856/1, Institute Strategic Programme Grants “Understanding and Exploiting Plant and Microbial Secondary Metabolism” (BB/J004596/1) and “Molecules from Nature – Enhanced Research Capacity” (BBS/E/J/000PR9794) and the John Innes Foundation. At VTT the work was supported by Ministry of Agriculture and Forestry (2145/312/2014) and VTT. We thank the Wellcome Trust for Ph.D. Studentship support to DLH (102572/B/13/Z).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Atlantic cod nervous necrosis virus
Protective immunity
Sea bass
Transient expression
Virus-like particles

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10.3389/fpls.2019.00880

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title = "Plant-made nervous necrosis virus-like particles protect fish against disease",

abstract = "Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.",

keywords = "Atlantic cod nervous necrosis virus, Protective immunity, Sea bass, Transient expression, Virus-like particles",

author = "Johanna Marsian and Hurdiss, \{Daniel L.\} and Ranson, \{Neil A.\} and Anneli Ritala and Richard Paley and Irene Cano and Lomonossoff, \{George P.\}",

year = "2019",

month = jul,

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doi = "10.3389/fpls.2019.00880",

language = "English",

volume = "10",

journal = "Frontiers in Plant Science",

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T1 - Plant-made nervous necrosis virus-like particles protect fish against disease

AU - Marsian, Johanna

AU - Hurdiss, Daniel L.

AU - Ranson, Neil A.

AU - Ritala, Anneli

AU - Paley, Richard

AU - Cano, Irene

AU - Lomonossoff, George P.

PY - 2019/7/9

Y1 - 2019/7/9

N2 - Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

AB - Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

KW - Atlantic cod nervous necrosis virus

KW - Protective immunity

KW - Sea bass

KW - Transient expression

KW - Virus-like particles

UR - https://www.scopus.com/pages/publications/85069469768

U2 - 10.3389/fpls.2019.00880

DO - 10.3389/fpls.2019.00880

M3 - Article

AN - SCOPUS:85069469768

SN - 1664-462X

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 880

ER -

Plant-made nervous necrosis virus-like particles protect fish against disease

Abstract

Funding

UN SDGs

Keywords

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