Plants utilize isoprene emission as a thermotolerance mechanism

K. Sasaki, T. Saito, Mari Lämsä, Kirsi-Marja Oksman-Caldentey, M. Suzuki, K. Ohyama, T. Muranaka, K. Ohara, K. Yazaki (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)

Abstract

Isoprene is a volatile compound emitted from leaves of many plant species in large quantities, which has an impact on atmospheric chemistry due to its massive global emission rate (5 × 1014 carbon g year−1) and its high reactivity with the OH radical, resulting in an increase in the half-life of methane.
Isoprene emission is strongly induced by the increase in isoprene synthase activity in plastids at high temperature in the day time, which is regulated at its gene expression level in leaves, while the physiological meaning of isoprene emission for plants has not been clearly demonstrated. In this study, we have functionally overexpressed Populus alba isoprene synthase in Arabidopsis to observe isoprene emission from transgenic plants.
A striking difference was observed when both transgenic and wild-type plants were treated with heat at 60°C for 2.5 h, i.e. transformants revealed clear heat tolerance compared with the wild type.
High isoprene emission and a decrease in the leaf surface temperature were observed in transgenic plants under heat stress treatment. In contrast, neither strong light nor drought treatments showed an apparent difference.
These data suggest that isoprene emission plays a crucial role in a heat protection mechanism in plants.
Original languageEnglish
Pages (from-to)1254-1262
JournalPlant and Cell Physiology
Volume48
Issue number9
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

heat tolerance
Genetically Modified Plants
Hot Temperature
Populus
transgenic plants
Temperature
Plastids
Plant Leaves
Droughts
Methane
atmospheric chemistry
heat
Arabidopsis
Populus alba
leaves
Half-Life
isoprene
Thermotolerance
Carbon
volatile compounds

Keywords

  • Arabidopsis
  • environmental stress
  • isoprene
  • physiological function
  • Populus alba
  • thermotolerance

Cite this

Sasaki, K., Saito, T., Lämsä, M., Oksman-Caldentey, K-M., Suzuki, M., Ohyama, K., ... Yazaki, K. (2007). Plants utilize isoprene emission as a thermotolerance mechanism. Plant and Cell Physiology, 48(9), 1254-1262. https://doi.org/10.1093/pcp/pcm104
Sasaki, K. ; Saito, T. ; Lämsä, Mari ; Oksman-Caldentey, Kirsi-Marja ; Suzuki, M. ; Ohyama, K. ; Muranaka, T. ; Ohara, K. ; Yazaki, K. / Plants utilize isoprene emission as a thermotolerance mechanism. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 9. pp. 1254-1262.
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abstract = "Isoprene is a volatile compound emitted from leaves of many plant species in large quantities, which has an impact on atmospheric chemistry due to its massive global emission rate (5 × 1014 carbon g year−1) and its high reactivity with the OH radical, resulting in an increase in the half-life of methane. Isoprene emission is strongly induced by the increase in isoprene synthase activity in plastids at high temperature in the day time, which is regulated at its gene expression level in leaves, while the physiological meaning of isoprene emission for plants has not been clearly demonstrated. In this study, we have functionally overexpressed Populus alba isoprene synthase in Arabidopsis to observe isoprene emission from transgenic plants. A striking difference was observed when both transgenic and wild-type plants were treated with heat at 60°C for 2.5 h, i.e. transformants revealed clear heat tolerance compared with the wild type. High isoprene emission and a decrease in the leaf surface temperature were observed in transgenic plants under heat stress treatment. In contrast, neither strong light nor drought treatments showed an apparent difference. These data suggest that isoprene emission plays a crucial role in a heat protection mechanism in plants.",
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Sasaki, K, Saito, T, Lämsä, M, Oksman-Caldentey, K-M, Suzuki, M, Ohyama, K, Muranaka, T, Ohara, K & Yazaki, K 2007, 'Plants utilize isoprene emission as a thermotolerance mechanism', Plant and Cell Physiology, vol. 48, no. 9, pp. 1254-1262. https://doi.org/10.1093/pcp/pcm104

Plants utilize isoprene emission as a thermotolerance mechanism. / Sasaki, K.; Saito, T.; Lämsä, Mari; Oksman-Caldentey, Kirsi-Marja; Suzuki, M.; Ohyama, K.; Muranaka, T.; Ohara, K.; Yazaki, K. (Corresponding Author).

In: Plant and Cell Physiology, Vol. 48, No. 9, 2007, p. 1254-1262.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Plants utilize isoprene emission as a thermotolerance mechanism

AU - Sasaki, K.

AU - Saito, T.

AU - Lämsä, Mari

AU - Oksman-Caldentey, Kirsi-Marja

AU - Suzuki, M.

AU - Ohyama, K.

AU - Muranaka, T.

AU - Ohara, K.

AU - Yazaki, K.

PY - 2007

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N2 - Isoprene is a volatile compound emitted from leaves of many plant species in large quantities, which has an impact on atmospheric chemistry due to its massive global emission rate (5 × 1014 carbon g year−1) and its high reactivity with the OH radical, resulting in an increase in the half-life of methane. Isoprene emission is strongly induced by the increase in isoprene synthase activity in plastids at high temperature in the day time, which is regulated at its gene expression level in leaves, while the physiological meaning of isoprene emission for plants has not been clearly demonstrated. In this study, we have functionally overexpressed Populus alba isoprene synthase in Arabidopsis to observe isoprene emission from transgenic plants. A striking difference was observed when both transgenic and wild-type plants were treated with heat at 60°C for 2.5 h, i.e. transformants revealed clear heat tolerance compared with the wild type. High isoprene emission and a decrease in the leaf surface temperature were observed in transgenic plants under heat stress treatment. In contrast, neither strong light nor drought treatments showed an apparent difference. These data suggest that isoprene emission plays a crucial role in a heat protection mechanism in plants.

AB - Isoprene is a volatile compound emitted from leaves of many plant species in large quantities, which has an impact on atmospheric chemistry due to its massive global emission rate (5 × 1014 carbon g year−1) and its high reactivity with the OH radical, resulting in an increase in the half-life of methane. Isoprene emission is strongly induced by the increase in isoprene synthase activity in plastids at high temperature in the day time, which is regulated at its gene expression level in leaves, while the physiological meaning of isoprene emission for plants has not been clearly demonstrated. In this study, we have functionally overexpressed Populus alba isoprene synthase in Arabidopsis to observe isoprene emission from transgenic plants. A striking difference was observed when both transgenic and wild-type plants were treated with heat at 60°C for 2.5 h, i.e. transformants revealed clear heat tolerance compared with the wild type. High isoprene emission and a decrease in the leaf surface temperature were observed in transgenic plants under heat stress treatment. In contrast, neither strong light nor drought treatments showed an apparent difference. These data suggest that isoprene emission plays a crucial role in a heat protection mechanism in plants.

KW - Arabidopsis

KW - environmental stress

KW - isoprene

KW - physiological function

KW - Populus alba

KW - thermotolerance

U2 - 10.1093/pcp/pcm104

DO - 10.1093/pcp/pcm104

M3 - Article

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SP - 1254

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JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

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ER -