Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria

Ilkka Helander (Corresponding Author), Hanna Alakomi, Kyösti Latva-Kala, Pertti Koski

Research output: Contribution to journalArticleScientificpeer-review

169 Citations (Scopus)

Abstract

The effect of the polycation polyethyleneimine (PEI) on the permeability properties of the Gram-negative bacterial outer membrane was investigated using Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium as target organisms. At concentrations of less than 20 μg ml-1, PEI increased the bacterial uptake of 1-N-phenylnaphthylamine, which is a hydrophobic probe whose quantum yield is greatly increased in a lipid environment, indicating increased hydrophobic permeation of the outer membrane by PEI. The effect of PEI was comparable to that brought about by the well-known permeabilizer EDTA. Permeabilization by PEI was retarded but not completely inhibited by millimolar concentrations of MgCl2. PEI also increased the susceptibility of the test species to the hydrophobic antibiotics clindamycin, erythromycin, fucidin, novobiocin and rifampicin, without being directly bactericidal. PEI sensitized the bacteria to the lytic action of the detergent SDS in assays where the bacteria were pretreated with PEI. In assays where PEI and SDS were simultaneously present, no sensitization was observed, indicating that PEI and SDS were inactivating each other. In addition, a sensitizing effect to the nonionic detergent Triton X-100 was observed for P. aeruginosa. In conclusion, PEI was shown to be a potent permeabilizer of the outer membrane of Gram-negative bacteria.

Original languageEnglish
Pages (from-to)3193 - 3199
Number of pages7
JournalMicrobiology
Volume143
Issue number10
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Polyethyleneimine
Gram-Negative Bacteria
N-phenyl-1-naphthylamine
Detergents
Pseudomonas aeruginosa
Membranes
Novobiocin
Bacteria
Magnesium Chloride
Clindamycin
Octoxynol
Erythromycin
Salmonella typhimurium
Rifampin
Edetic Acid
Permeability

Cite this

Helander, I., Alakomi, H., Latva-Kala, K., & Koski, P. (1997). Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria. Microbiology, 143(10), 3193 - 3199. https://doi.org/10.1099/00221287-143-10-3193
Helander, Ilkka ; Alakomi, Hanna ; Latva-Kala, Kyösti ; Koski, Pertti. / Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria. In: Microbiology. 1997 ; Vol. 143, No. 10. pp. 3193 - 3199.
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Helander, I, Alakomi, H, Latva-Kala, K & Koski, P 1997, 'Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria', Microbiology, vol. 143, no. 10, pp. 3193 - 3199. https://doi.org/10.1099/00221287-143-10-3193

Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria. / Helander, Ilkka (Corresponding Author); Alakomi, Hanna; Latva-Kala, Kyösti; Koski, Pertti.

In: Microbiology, Vol. 143, No. 10, 1997, p. 3193 - 3199.

Research output: Contribution to journalArticleScientificpeer-review

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