Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial

Reetta Satokari, Elaine Vaughan, Antoon Akkermans, Maria Saarela, Willem de Vos

Research output: Contribution to journalArticleScientificpeer-review

78 Citations (Scopus)

Abstract

A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 × 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalSystematic and Applied Microbiology
Volume24
Issue number2
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Prebiotics
Denaturing Gradient Gel Electrophoresis
Bifidobacterium
probiotics
Probiotics
denaturing gradient gel electrophoresis
prebiotics
polymerase chain reaction
electrokinesis
gel
Polymerase Chain Reaction
monitoring
Population
galactooligosaccharides
indigenous population
Ribosomal DNA
Population Groups
Feces
feces
digestive system

Cite this

Satokari, Reetta ; Vaughan, Elaine ; Akkermans, Antoon ; Saarela, Maria ; de Vos, Willem. / Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial. In: Systematic and Applied Microbiology. 2001 ; Vol. 24, No. 2. pp. 227-231.
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abstract = "A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 × 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.",
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Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial. / Satokari, Reetta; Vaughan, Elaine; Akkermans, Antoon; Saarela, Maria; de Vos, Willem.

In: Systematic and Applied Microbiology, Vol. 24, No. 2, 2001, p. 227-231.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Saarela, Maria

AU - de Vos, Willem

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AB - A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 × 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.

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