Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 227-231 |
| Number of pages | 5 |
| Journal | Systematic and Applied Microbiology |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2001 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Cite this
}
Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial. / Satokari, Reetta; Vaughan, Elaine; Akkermans, Antoon; Saarela, Maria; de Vos, Willem.
In: Systematic and Applied Microbiology, Vol. 24, No. 2, 2001, p. 227-231.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial
AU - Satokari, Reetta
AU - Vaughan, Elaine
AU - Akkermans, Antoon
AU - Saarela, Maria
AU - de Vos, Willem
PY - 2001
Y1 - 2001
N2 - A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 × 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
AB - A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human feeding trial. DNA was extracted directly from feces and bifidobacterial 16S rDNA sequences were amplified using genus-specific PCR. The PCR fragments were subsequently separated in a sequence-specific manner by DGGE in order to obtain a profile of bifidobacterial fragments. The DGGE profiles revealed that in general, administration for two weeks of galactooligosaccharide and/or Bifidobacterium lactis Bb-12 (8 g and 3 × 1010 cfu per day, respectively) did not affect the qualitative composition of the indigenous Bifidobacterium population, while B. lactis Bb-12 transiently colonised the gut.
U2 - 10.1078/0723-2020-00035
DO - 10.1078/0723-2020-00035
M3 - Article
VL - 24
SP - 227
EP - 231
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
SN - 0723-2020
IS - 2
ER -