Polymerisation of added coniferyl alcohol by inherent xylem peroxidases and its effect on fungal decay resistance of Norway spruce

Sanni Raiskila, Kurt Fagerstedt (Corresponding Author), Tapio Laakso, Pekka Saranpää, Mia Löija, Leena Paajanen, Riitta Mahlberg, Anne-Christine Ritschkoff

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

It is known that active peroxidase isozymes exist in mature wood of Norway spruce (Picea abies L. Karst.) and that they remain active for years and are found even in the heartwood (in Scots pine), where all cellular activity has ceased. This peroxidase activity was utilised in the impregnation of wood blocks with a natural monolignol, coniferyl alcohol and hydrogen peroxide. The hypothesis was that the internal wood peroxidases would oxidise the added monolignol and bind it stably into the cell wall matrix, which could hinder fungal decay. Since coniferyl alcohol is not very soluble in water, the impregnation was done under vacuum with an acetone–water solution containing 10% coniferyl alcohol and 0.4 mM H2O2 at room temperature (ca. 0.02 g of coniferyl alcohol was added to 1 g of wood). After impregnation, dimers of coniferyl alcohol and free coniferyl alcohol were found in acetone extracts with GC–MS analysis. Penetration of coniferyl alcohol and non-extractable reaction products were studied from the wood blocks with FTIR PAS technique. The wood samples treated were also subjected to a fungal decay test with Coriolus versicolor. This treatment hindered fungal decay in a 60-day experiment and led to a dry weight loss of 8.8% in comparison with 19.9% in the control. The reactions of coniferyl alcohol and H2O2 in the presence of peroxidases are discussed as well as the use of monolignols to increase wood decay resistance.
Original languageEnglish
Pages (from-to)697-707
JournalWood Science and Technology
Volume40
Issue number8
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

decay resistance
coniferyl alcohol
Peroxidases
peroxidases
xylem
polymerization
Picea abies
alcohol
Alcohols
Polymerization
Wood
Impregnation
deterioration
Peroxidase
peroxidase
Decay (organic)
Coriolus versicolor
decayed wood
effect
heartwood

Keywords

  • coniferyl alcohol
  • decay resistance
  • wood

Cite this

Raiskila, Sanni ; Fagerstedt, Kurt ; Laakso, Tapio ; Saranpää, Pekka ; Löija, Mia ; Paajanen, Leena ; Mahlberg, Riitta ; Ritschkoff, Anne-Christine. / Polymerisation of added coniferyl alcohol by inherent xylem peroxidases and its effect on fungal decay resistance of Norway spruce. In: Wood Science and Technology. 2006 ; Vol. 40, No. 8. pp. 697-707.
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abstract = "It is known that active peroxidase isozymes exist in mature wood of Norway spruce (Picea abies L. Karst.) and that they remain active for years and are found even in the heartwood (in Scots pine), where all cellular activity has ceased. This peroxidase activity was utilised in the impregnation of wood blocks with a natural monolignol, coniferyl alcohol and hydrogen peroxide. The hypothesis was that the internal wood peroxidases would oxidise the added monolignol and bind it stably into the cell wall matrix, which could hinder fungal decay. Since coniferyl alcohol is not very soluble in water, the impregnation was done under vacuum with an acetone–water solution containing 10{\%} coniferyl alcohol and 0.4 mM H2O2 at room temperature (ca. 0.02 g of coniferyl alcohol was added to 1 g of wood). After impregnation, dimers of coniferyl alcohol and free coniferyl alcohol were found in acetone extracts with GC–MS analysis. Penetration of coniferyl alcohol and non-extractable reaction products were studied from the wood blocks with FTIR PAS technique. The wood samples treated were also subjected to a fungal decay test with Coriolus versicolor. This treatment hindered fungal decay in a 60-day experiment and led to a dry weight loss of 8.8{\%} in comparison with 19.9{\%} in the control. The reactions of coniferyl alcohol and H2O2 in the presence of peroxidases are discussed as well as the use of monolignols to increase wood decay resistance.",
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Polymerisation of added coniferyl alcohol by inherent xylem peroxidases and its effect on fungal decay resistance of Norway spruce. / Raiskila, Sanni; Fagerstedt, Kurt (Corresponding Author); Laakso, Tapio; Saranpää, Pekka; Löija, Mia; Paajanen, Leena; Mahlberg, Riitta; Ritschkoff, Anne-Christine.

In: Wood Science and Technology, Vol. 40, No. 8, 2006, p. 697-707.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Polymerisation of added coniferyl alcohol by inherent xylem peroxidases and its effect on fungal decay resistance of Norway spruce

AU - Raiskila, Sanni

AU - Fagerstedt, Kurt

AU - Laakso, Tapio

AU - Saranpää, Pekka

AU - Löija, Mia

AU - Paajanen, Leena

AU - Mahlberg, Riitta

AU - Ritschkoff, Anne-Christine

PY - 2006

Y1 - 2006

N2 - It is known that active peroxidase isozymes exist in mature wood of Norway spruce (Picea abies L. Karst.) and that they remain active for years and are found even in the heartwood (in Scots pine), where all cellular activity has ceased. This peroxidase activity was utilised in the impregnation of wood blocks with a natural monolignol, coniferyl alcohol and hydrogen peroxide. The hypothesis was that the internal wood peroxidases would oxidise the added monolignol and bind it stably into the cell wall matrix, which could hinder fungal decay. Since coniferyl alcohol is not very soluble in water, the impregnation was done under vacuum with an acetone–water solution containing 10% coniferyl alcohol and 0.4 mM H2O2 at room temperature (ca. 0.02 g of coniferyl alcohol was added to 1 g of wood). After impregnation, dimers of coniferyl alcohol and free coniferyl alcohol were found in acetone extracts with GC–MS analysis. Penetration of coniferyl alcohol and non-extractable reaction products were studied from the wood blocks with FTIR PAS technique. The wood samples treated were also subjected to a fungal decay test with Coriolus versicolor. This treatment hindered fungal decay in a 60-day experiment and led to a dry weight loss of 8.8% in comparison with 19.9% in the control. The reactions of coniferyl alcohol and H2O2 in the presence of peroxidases are discussed as well as the use of monolignols to increase wood decay resistance.

AB - It is known that active peroxidase isozymes exist in mature wood of Norway spruce (Picea abies L. Karst.) and that they remain active for years and are found even in the heartwood (in Scots pine), where all cellular activity has ceased. This peroxidase activity was utilised in the impregnation of wood blocks with a natural monolignol, coniferyl alcohol and hydrogen peroxide. The hypothesis was that the internal wood peroxidases would oxidise the added monolignol and bind it stably into the cell wall matrix, which could hinder fungal decay. Since coniferyl alcohol is not very soluble in water, the impregnation was done under vacuum with an acetone–water solution containing 10% coniferyl alcohol and 0.4 mM H2O2 at room temperature (ca. 0.02 g of coniferyl alcohol was added to 1 g of wood). After impregnation, dimers of coniferyl alcohol and free coniferyl alcohol were found in acetone extracts with GC–MS analysis. Penetration of coniferyl alcohol and non-extractable reaction products were studied from the wood blocks with FTIR PAS technique. The wood samples treated were also subjected to a fungal decay test with Coriolus versicolor. This treatment hindered fungal decay in a 60-day experiment and led to a dry weight loss of 8.8% in comparison with 19.9% in the control. The reactions of coniferyl alcohol and H2O2 in the presence of peroxidases are discussed as well as the use of monolignols to increase wood decay resistance.

KW - coniferyl alcohol

KW - decay resistance

KW - wood

U2 - 10.1007/s00226-006-0081-x

DO - 10.1007/s00226-006-0081-x

M3 - Article

VL - 40

SP - 697

EP - 707

JO - Wood Science and Technology

JF - Wood Science and Technology

SN - 0043-7719

IS - 8

ER -