Polymerization of different lignins by laccase

Maija-Liisa Mattinen, Tapani Suortti, R. Gosseling, D. S. Argyropoulos, D. Evtuguin, Anna Suurnäkki, E. de Jong, Tarja Tamminen

Research output: Contribution to journalArticleScientificpeer-review

55 Citations (Scopus)

Abstract

In this study the oxidative polymerization of different lignins, i.e. Flax Soda lignin, Spruce EMAL, and Eucalyptus Dioxane lignin by Trametes hirsuta laccase was compared. Initially the structures of the different lignins were compared by Fourier transform infrared spectroscopy. The reactivity of laccase with the different types of lignins in the absence of mediators was examined and verified by oxygen consumption measurements. The molecular weight distributions of treated and untreated lignins were determined by two different size exclusion chromatography methods. Furthermore, the potential of matrix-assisted laser desorption/ionisation-time of flight-mass spectroscopy for determination of the absolute molecular weights of the different lignins was evaluated. The data showed that all the technical lignins could be activated and polymerized by laccase to different degrees. The efficiency as indicated by measurements of the degree of polymerization was found to increase in the order of Spruce EMAL < Eucalyptus Dioxane lignin < Flax Soda lignin. Overall, this data supplies foundations for using enzymes more efficiently in the enzymatic upgrading of lignin.
Original languageEnglish
Pages (from-to)549-565
Number of pages17
JournalBioResources
Volume3
Issue number2
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
polymerization
lignin
Polymerization
Flax
Size exclusion chromatography
Molecular weight distribution
oxygen consumption
FTIR spectroscopy
chromatography
Ionization
Fourier transform infrared spectroscopy
Desorption
desorption
ionization
Enzymes
laser
Molecular weight
spectroscopy

Keywords

  • Lignin
  • laccase
  • molecular weight distribution
  • polymerization
  • SEC
  • MALDI-TOF MS

Cite this

Mattinen, M-L., Suortti, T., Gosseling, R., Argyropoulos, D. S., Evtuguin, D., Suurnäkki, A., ... Tamminen, T. (2008). Polymerization of different lignins by laccase. BioResources, 3(2), 549-565.
Mattinen, Maija-Liisa ; Suortti, Tapani ; Gosseling, R. ; Argyropoulos, D. S. ; Evtuguin, D. ; Suurnäkki, Anna ; de Jong, E. ; Tamminen, Tarja. / Polymerization of different lignins by laccase. In: BioResources. 2008 ; Vol. 3, No. 2. pp. 549-565.
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Mattinen, M-L, Suortti, T, Gosseling, R, Argyropoulos, DS, Evtuguin, D, Suurnäkki, A, de Jong, E & Tamminen, T 2008, 'Polymerization of different lignins by laccase', BioResources, vol. 3, no. 2, pp. 549-565.

Polymerization of different lignins by laccase. / Mattinen, Maija-Liisa; Suortti, Tapani; Gosseling, R.; Argyropoulos, D. S.; Evtuguin, D.; Suurnäkki, Anna; de Jong, E.; Tamminen, Tarja.

In: BioResources, Vol. 3, No. 2, 2008, p. 549-565.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Polymerization of different lignins by laccase

AU - Mattinen, Maija-Liisa

AU - Suortti, Tapani

AU - Gosseling, R.

AU - Argyropoulos, D. S.

AU - Evtuguin, D.

AU - Suurnäkki, Anna

AU - de Jong, E.

AU - Tamminen, Tarja

PY - 2008

Y1 - 2008

N2 - In this study the oxidative polymerization of different lignins, i.e. Flax Soda lignin, Spruce EMAL, and Eucalyptus Dioxane lignin by Trametes hirsuta laccase was compared. Initially the structures of the different lignins were compared by Fourier transform infrared spectroscopy. The reactivity of laccase with the different types of lignins in the absence of mediators was examined and verified by oxygen consumption measurements. The molecular weight distributions of treated and untreated lignins were determined by two different size exclusion chromatography methods. Furthermore, the potential of matrix-assisted laser desorption/ionisation-time of flight-mass spectroscopy for determination of the absolute molecular weights of the different lignins was evaluated. The data showed that all the technical lignins could be activated and polymerized by laccase to different degrees. The efficiency as indicated by measurements of the degree of polymerization was found to increase in the order of Spruce EMAL < Eucalyptus Dioxane lignin < Flax Soda lignin. Overall, this data supplies foundations for using enzymes more efficiently in the enzymatic upgrading of lignin.

AB - In this study the oxidative polymerization of different lignins, i.e. Flax Soda lignin, Spruce EMAL, and Eucalyptus Dioxane lignin by Trametes hirsuta laccase was compared. Initially the structures of the different lignins were compared by Fourier transform infrared spectroscopy. The reactivity of laccase with the different types of lignins in the absence of mediators was examined and verified by oxygen consumption measurements. The molecular weight distributions of treated and untreated lignins were determined by two different size exclusion chromatography methods. Furthermore, the potential of matrix-assisted laser desorption/ionisation-time of flight-mass spectroscopy for determination of the absolute molecular weights of the different lignins was evaluated. The data showed that all the technical lignins could be activated and polymerized by laccase to different degrees. The efficiency as indicated by measurements of the degree of polymerization was found to increase in the order of Spruce EMAL < Eucalyptus Dioxane lignin < Flax Soda lignin. Overall, this data supplies foundations for using enzymes more efficiently in the enzymatic upgrading of lignin.

KW - Lignin

KW - laccase

KW - molecular weight distribution

KW - polymerization

KW - SEC

KW - MALDI-TOF MS

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JO - BioResources

JF - BioResources

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Mattinen M-L, Suortti T, Gosseling R, Argyropoulos DS, Evtuguin D, Suurnäkki A et al. Polymerization of different lignins by laccase. BioResources. 2008;3(2):549-565.