Polypeptide purification with industrial-scale supercritical fluid chromatography

Olli Aaltonen, Martti Alkio, Juhani Lundell, Sauli Ruohonen, Lasse Parvinen, Veikko Suoninen

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.
Original languageEnglish
Pages (from-to)XLII-LIV
JournalPharmaceutical Technology Europe
Volume10
Issue number9
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Supercritical Fluid Chromatography
Cyclosporine
Chlorinated Hydrocarbons
Peptides
Crystallization
Fermentation

Cite this

Aaltonen, O., Alkio, M., Lundell, J., Ruohonen, S., Parvinen, L., & Suoninen, V. (1998). Polypeptide purification with industrial-scale supercritical fluid chromatography. Pharmaceutical Technology Europe, 10(9), XLII-LIV.
Aaltonen, Olli ; Alkio, Martti ; Lundell, Juhani ; Ruohonen, Sauli ; Parvinen, Lasse ; Suoninen, Veikko. / Polypeptide purification with industrial-scale supercritical fluid chromatography. In: Pharmaceutical Technology Europe. 1998 ; Vol. 10, No. 9. pp. XLII-LIV.
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Aaltonen, O, Alkio, M, Lundell, J, Ruohonen, S, Parvinen, L & Suoninen, V 1998, 'Polypeptide purification with industrial-scale supercritical fluid chromatography', Pharmaceutical Technology Europe, vol. 10, no. 9, pp. XLII-LIV.

Polypeptide purification with industrial-scale supercritical fluid chromatography. / Aaltonen, Olli; Alkio, Martti; Lundell, Juhani; Ruohonen, Sauli; Parvinen, Lasse; Suoninen, Veikko.

In: Pharmaceutical Technology Europe, Vol. 10, No. 9, 1998, p. XLII-LIV.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Polypeptide purification with industrial-scale supercritical fluid chromatography

AU - Aaltonen, Olli

AU - Alkio, Martti

AU - Lundell, Juhani

AU - Ruohonen, Sauli

AU - Parvinen, Lasse

AU - Suoninen, Veikko

PY - 1998

Y1 - 1998

N2 - This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.

AB - This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.

M3 - Article

VL - 10

SP - XLII-LIV

JO - Pharmaceutical Technology Europe

JF - Pharmaceutical Technology Europe

SN - 1753-7967

IS - 9

ER -

Aaltonen O, Alkio M, Lundell J, Ruohonen S, Parvinen L, Suoninen V. Polypeptide purification with industrial-scale supercritical fluid chromatography. Pharmaceutical Technology Europe. 1998;10(9):XLII-LIV.