Polypeptide purification with industrial-scale supercritical fluid chromatography

Olli Aaltonen, Martti Alkio, Juhani Lundell, Sauli Ruohonen, Lasse Parvinen, Veikko Suoninen

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)


This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.
Original languageEnglish
Pages (from-to)XLII-LIV
JournalPharmaceutical Technology Europe
Issue number9
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed


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