This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.
|Pharmaceutical Technology Europe
|Published - 1998
|MoE publication type
|A1 Journal article-refereed