This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.
|Journal||Pharmaceutical Technology Europe|
|Publication status||Published - 1998|
|MoE publication type||A1 Journal article-refereed|
Aaltonen, O., Alkio, M., Lundell, J., Ruohonen, S., Parvinen, L., & Suoninen, V. (1998). Polypeptide purification with industrial-scale supercritical fluid chromatography. Pharmaceutical Technology Europe, 10(9), XLII-LIV.