Polypeptide purification with industrial-scale supercritical fluid chromatography

Olli Aaltonen; Martti Alkio; Juhani Lundell; Sauli Ruohonen; Lasse Parvinen; Veikko Suoninen

Polypeptide purification with industrial-scale supercritical fluid chromatography

Olli Aaltonen
, Martti Alkio
, Juhani Lundell
, Sauli Ruohonen
, Lasse Parvinen
, Veikko Suoninen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.

Original language	English
Pages (from-to)	XLII-LIV
Journal	Pharmaceutical Technology Europe
Volume	10
Issue number	9
Publication status	Published - 1998
MoE publication type	A1 Journal article-refereed

Cite this

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title = "Polypeptide purification with industrial-scale supercritical fluid chromatography",

abstract = "This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.",

author = "Olli Aaltonen and Martti Alkio and Juhani Lundell and Sauli Ruohonen and Lasse Parvinen and Veikko Suoninen",

year = "1998",

language = "English",

volume = "10",

pages = "XLII--LIV",

journal = "Pharmaceutical Technology Europe",

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T1 - Polypeptide purification with industrial-scale supercritical fluid chromatography

AU - Aaltonen, Olli

AU - Alkio, Martti

AU - Lundell, Juhani

AU - Ruohonen, Sauli

AU - Parvinen, Lasse

AU - Suoninen, Veikko

PY - 1998

Y1 - 1998

N2 - This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.

AB - This article describes a supercritical fluid chromatography (SFC) process for separating a polypeptide from a mycelial extract, in this case, the purification of cyclosporin A obtained by fermentation. The chromatographic challenge was to separate a number of very similar cyclosporin forms to produce a product for final crystallization, and the technological challenge was to develop a compact, rapid process that would not use chlorinated or hydrocarbon solvents.

M3 - Article

SN - 1753-7967

VL - 10

SP - XLII-LIV

JO - Pharmaceutical Technology Europe

JF - Pharmaceutical Technology Europe

IS - 9

ER -

Polypeptide purification with industrial-scale supercritical fluid chromatography

Abstract

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