Polypyrrole coating on poly-(lactide/glycolide)-ß-tricalcium phosphate screws enhances new bone formation in rabbits

Ming-Dong Zhao, Miina Björninen, Lu Cao, Hui-Ren Wang, Jani Pelto, Xiang-Qian Li, Jari Hyttinen, Yun-Qi Jiang, Minna Kellomäki, Susanna Miettinen, George K. Sándor, Riitta Seppänen, Suvi Haimi, Jian Dong

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Polypyrrole (PPy) has gained interest as an implant material due to its multifunctional properties and its high compatibility with several cell and tissue types. For the first time, the biocompatibility and osteointegration of PPy coating, incorporated with chondroitin sulfate (CS), were studied in vivo by implanting PPy-coated bioabsorbable bone fixation composite screws of poly-(lactide/glycolide) copolymer (PLGA) and ß-tricalcium phosphate (TCP) into New Zealand white rabbits. Uncoated bioabsorbable polymer composite screws and commercially available stainless steel cortical screws were used as reference implants. The rabbits were euthanized 12 and 26 weeks after the implantation. The systemic effects were evaluated from food and water consumption, body weight, body temperature, clinical signs, blood samples, internal organ weights, and histological examination. Local effects were studied from bone tissue and surrounding soft tissue histology. New bone formation was evaluated by micro-computed tomography, tetracycline labeling and torsion tests. Torsion tests were performed in order to capture the peak value of the torsion force during the course of the screw's loosening. The coated screws induced significantly more bone formation than the uncoated screws. In addition, none of the implants induced any systemic or local toxicity. The results suggest that PPy is biocompatible with bone tissue and is a potential coating for enhancing osteointegration in orthopedic implants.
Original languageEnglish
Article number065016
JournalBiomedical Materials
Volume10
Issue number6
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Polypyrroles
Bone
Phosphates
Coatings
Torsional stress
Tissue
Polyglactin 910
Histology
Chondroitin Sulfates
Stainless Steel
Composite materials
Orthopedics
Tetracycline
Biocompatibility
Labeling
Tomography
Toxicity
polypyrrole
poly(lactide)
tricalcium phosphate

Keywords

  • absorbable screw
  • biocompatibility
  • in vivo
  • osteointegration
  • polypyrrole (PPy)

Cite this

Zhao, Ming-Dong ; Björninen, Miina ; Cao, Lu ; Wang, Hui-Ren ; Pelto, Jani ; Li, Xiang-Qian ; Hyttinen, Jari ; Jiang, Yun-Qi ; Kellomäki, Minna ; Miettinen, Susanna ; Sándor, George K. ; Seppänen, Riitta ; Haimi, Suvi ; Dong, Jian. / Polypyrrole coating on poly-(lactide/glycolide)-ß-tricalcium phosphate screws enhances new bone formation in rabbits. In: Biomedical Materials. 2015 ; Vol. 10, No. 6.
@article{47e3203e915f4ca1bbae1b7ffc3f862e,
title = "Polypyrrole coating on poly-(lactide/glycolide)-{\ss}-tricalcium phosphate screws enhances new bone formation in rabbits",
abstract = "Polypyrrole (PPy) has gained interest as an implant material due to its multifunctional properties and its high compatibility with several cell and tissue types. For the first time, the biocompatibility and osteointegration of PPy coating, incorporated with chondroitin sulfate (CS), were studied in vivo by implanting PPy-coated bioabsorbable bone fixation composite screws of poly-(lactide/glycolide) copolymer (PLGA) and {\ss}-tricalcium phosphate (TCP) into New Zealand white rabbits. Uncoated bioabsorbable polymer composite screws and commercially available stainless steel cortical screws were used as reference implants. The rabbits were euthanized 12 and 26 weeks after the implantation. The systemic effects were evaluated from food and water consumption, body weight, body temperature, clinical signs, blood samples, internal organ weights, and histological examination. Local effects were studied from bone tissue and surrounding soft tissue histology. New bone formation was evaluated by micro-computed tomography, tetracycline labeling and torsion tests. Torsion tests were performed in order to capture the peak value of the torsion force during the course of the screw's loosening. The coated screws induced significantly more bone formation than the uncoated screws. In addition, none of the implants induced any systemic or local toxicity. The results suggest that PPy is biocompatible with bone tissue and is a potential coating for enhancing osteointegration in orthopedic implants.",
keywords = "absorbable screw, biocompatibility, in vivo, osteointegration, polypyrrole (PPy)",
author = "Ming-Dong Zhao and Miina Bj{\"o}rninen and Lu Cao and Hui-Ren Wang and Jani Pelto and Xiang-Qian Li and Jari Hyttinen and Yun-Qi Jiang and Minna Kellom{\"a}ki and Susanna Miettinen and S{\'a}ndor, {George K.} and Riitta Sepp{\"a}nen and Suvi Haimi and Jian Dong",
year = "2015",
doi = "10.1088/1748-6041/10/6/065016",
language = "English",
volume = "10",
journal = "Biomedical Materials",
issn = "1748-6041",
publisher = "Institute of Physics IOP",
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Zhao, M-D, Björninen, M, Cao, L, Wang, H-R, Pelto, J, Li, X-Q, Hyttinen, J, Jiang, Y-Q, Kellomäki, M, Miettinen, S, Sándor, GK, Seppänen, R, Haimi, S & Dong, J 2015, 'Polypyrrole coating on poly-(lactide/glycolide)-ß-tricalcium phosphate screws enhances new bone formation in rabbits', Biomedical Materials, vol. 10, no. 6, 065016. https://doi.org/10.1088/1748-6041/10/6/065016

Polypyrrole coating on poly-(lactide/glycolide)-ß-tricalcium phosphate screws enhances new bone formation in rabbits. / Zhao, Ming-Dong; Björninen, Miina; Cao, Lu; Wang, Hui-Ren; Pelto, Jani; Li, Xiang-Qian; Hyttinen, Jari; Jiang, Yun-Qi; Kellomäki, Minna; Miettinen, Susanna; Sándor, George K.; Seppänen, Riitta; Haimi, Suvi; Dong, Jian.

In: Biomedical Materials, Vol. 10, No. 6, 065016, 2015.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Polypyrrole coating on poly-(lactide/glycolide)-ß-tricalcium phosphate screws enhances new bone formation in rabbits

AU - Zhao, Ming-Dong

AU - Björninen, Miina

AU - Cao, Lu

AU - Wang, Hui-Ren

AU - Pelto, Jani

AU - Li, Xiang-Qian

AU - Hyttinen, Jari

AU - Jiang, Yun-Qi

AU - Kellomäki, Minna

AU - Miettinen, Susanna

AU - Sándor, George K.

AU - Seppänen, Riitta

AU - Haimi, Suvi

AU - Dong, Jian

PY - 2015

Y1 - 2015

N2 - Polypyrrole (PPy) has gained interest as an implant material due to its multifunctional properties and its high compatibility with several cell and tissue types. For the first time, the biocompatibility and osteointegration of PPy coating, incorporated with chondroitin sulfate (CS), were studied in vivo by implanting PPy-coated bioabsorbable bone fixation composite screws of poly-(lactide/glycolide) copolymer (PLGA) and ß-tricalcium phosphate (TCP) into New Zealand white rabbits. Uncoated bioabsorbable polymer composite screws and commercially available stainless steel cortical screws were used as reference implants. The rabbits were euthanized 12 and 26 weeks after the implantation. The systemic effects were evaluated from food and water consumption, body weight, body temperature, clinical signs, blood samples, internal organ weights, and histological examination. Local effects were studied from bone tissue and surrounding soft tissue histology. New bone formation was evaluated by micro-computed tomography, tetracycline labeling and torsion tests. Torsion tests were performed in order to capture the peak value of the torsion force during the course of the screw's loosening. The coated screws induced significantly more bone formation than the uncoated screws. In addition, none of the implants induced any systemic or local toxicity. The results suggest that PPy is biocompatible with bone tissue and is a potential coating for enhancing osteointegration in orthopedic implants.

AB - Polypyrrole (PPy) has gained interest as an implant material due to its multifunctional properties and its high compatibility with several cell and tissue types. For the first time, the biocompatibility and osteointegration of PPy coating, incorporated with chondroitin sulfate (CS), were studied in vivo by implanting PPy-coated bioabsorbable bone fixation composite screws of poly-(lactide/glycolide) copolymer (PLGA) and ß-tricalcium phosphate (TCP) into New Zealand white rabbits. Uncoated bioabsorbable polymer composite screws and commercially available stainless steel cortical screws were used as reference implants. The rabbits were euthanized 12 and 26 weeks after the implantation. The systemic effects were evaluated from food and water consumption, body weight, body temperature, clinical signs, blood samples, internal organ weights, and histological examination. Local effects were studied from bone tissue and surrounding soft tissue histology. New bone formation was evaluated by micro-computed tomography, tetracycline labeling and torsion tests. Torsion tests were performed in order to capture the peak value of the torsion force during the course of the screw's loosening. The coated screws induced significantly more bone formation than the uncoated screws. In addition, none of the implants induced any systemic or local toxicity. The results suggest that PPy is biocompatible with bone tissue and is a potential coating for enhancing osteointegration in orthopedic implants.

KW - absorbable screw

KW - biocompatibility

KW - in vivo

KW - osteointegration

KW - polypyrrole (PPy)

U2 - 10.1088/1748-6041/10/6/065016

DO - 10.1088/1748-6041/10/6/065016

M3 - Article

VL - 10

JO - Biomedical Materials

JF - Biomedical Materials

SN - 1748-6041

IS - 6

M1 - 065016

ER -