Abstract
Development of novel enzymatic methods for slime deposit control in
paper mills requires knowledge of polysaccharide-producing organisms and
the polysaccharide structures present in deposits. In this work, 27
polysaccharide-producing bacteria were isolated from slime samples
collected from different parts of a paper machine. Most of the isolates
produced polysaccharides in liquid culture and nine of them were
selected for production of polysaccharides for characterisation. The
selected isolates belonged to seven different genera: Bacillus, Brevundimonas, Cytophaga, Enterobacter, Klebsiella, Paenibacillus and Starkeya. Using ribotyping, partial 16S rDNA sequencing, physiological tests and fatty acid analysis, four of the nine isolates: Bacillus cereus, Brevundimonas vesicularis, K. pneumoniae and P. stellifer were identified to the species level. Production of polysaccharides by the selected isolates varied between 0.07 and 1.20 g L−1, the highest amount being produced by B. vesicularis.
The polysaccharides were heteropolysaccharides with varying proportions
of galactose, glucose mannose, rhamnose fucose and uronic acids.
Original language | English |
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Pages (from-to) | 109 - 114 |
Number of pages | 6 |
Journal | Journal of industrial microbiology and biotechnology |
Volume | 32 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2005 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Paper machine
- Slime
- Bacterial polysaccharide
- Exopolysaccharide
- Biofilm