Polysaccharide-producing bacteria isolated from paper machine slime deposits

Marjaana Rättö (Corresponding Author), Maija-Liisa Suihko, Matti Siika-aho

    Research output: Contribution to journalArticleScientificpeer-review

    27 Citations (Scopus)

    Abstract

    Development of novel enzymatic methods for slime deposit control in paper mills requires knowledge of polysaccharide-producing organisms and the polysaccharide structures present in deposits. In this work, 27 polysaccharide-producing bacteria were isolated from slime samples collected from different parts of a paper machine. Most of the isolates produced polysaccharides in liquid culture and nine of them were selected for production of polysaccharides for characterisation. The selected isolates belonged to seven different genera: Bacillus, Brevundimonas, Cytophaga, Enterobacter, Klebsiella, Paenibacillus and Starkeya. Using ribotyping, partial 16S rDNA sequencing, physiological tests and fatty acid analysis, four of the nine isolates: Bacillus cereus, Brevundimonas vesicularis, K. pneumoniae and P. stellifer were identified to the species level. Production of polysaccharides by the selected isolates varied between 0.07 and 1.20 g L−1, the highest amount being produced by B. vesicularis. The polysaccharides were heteropolysaccharides with varying proportions of galactose, glucose mannose, rhamnose fucose and uronic acids.
    Original languageEnglish
    Pages (from-to)109 - 114
    Number of pages6
    JournalJournal of industrial microbiology and biotechnology
    Volume32
    Issue number3
    DOIs
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Paper machine
    • Slime
    • Bacterial polysaccharide
    • Exopolysaccharide
    • Biofilm

    Fingerprint

    Dive into the research topics of 'Polysaccharide-producing bacteria isolated from paper machine slime deposits'. Together they form a unique fingerprint.

    Cite this