Portable Low-Cost Fluorescence Reader for LFA Measurements

Arttu Huttunen, Sanna Aikio, Marika Kurkinen, Jukka Tapani Mäkinen, Risto Mitikka, Liisa Kivimäki, Marja Harjumaa, Janne Takalo-Mattila, Christina Liedert, Jussi Hiltunen, Leena Hakalahti

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)


A portable device for measuring fluorescence signals from a lateral flow test strip was developed. The aim was to build an easy-to-use, affordable reader device for fast, quantitative analysis of point-of-care tests, made mostly of commercial off-the-shelf components. The device is capable of exciting fluorescent labels with 532 nm and 650 nm lasers and collecting the emitted fluorescent light with a camera. Core of the device is a Raspberry Pi Zero W wireless single board computer that controls the Raspberry Pi camera v2 module and lasers. The computer can also perform the image analysis and communicate the results to a mobile phone application acting as a user interface. Sensitivity of the device was characterized by sweeping the imaging parameters and comparing the performance with a high-grade fluorescence reader. Using dummy LFA samples, it was found that the device is able to detect signals from analyte concentrations that are relevant in biological samples. Image exposure time of 500~μ s was enough to read a distinguishable signal. The manufacturing costs were estimated to be below 100 euros per device in mass production.
Original languageEnglish
Article number9088161
Pages (from-to)10275-10282
JournalIEEE Sensors Journal
Issue number17
Publication statusPublished - 1 Sept 2020
MoE publication typeA1 Journal article-refereed


This work was supported in part by the projects Home-CRP and the Printocent10 funded by Business Finland and European Regional Development Fund under Grant 3252/31/2015 and under Grant A73286 in part by the Academy of Finland Research Infrastructure Printed Intelligence Infrastructure (PII-FIRI) under Grant 320020, and in part by the Academy of Finland Flagship Programme, Photonics Research and Innovation (PREIN) decision under Grant 320168. The associate editor coordinating the review of this article and approving it for publication was Dr. Ying Zhang. (Corresponding author: Arttu Huttunen.) The authors are with the VTT Technical Research Centre of Finland Ltd., 90590 Oulu, Finland (e-mail: arttu.huttunen@vtt.fi). Digital Object Identifier 10.1109/JSEN.2020.2992894


  • Biosensor
  • fluorescent label
  • image sensors
  • LFA
  • POC
  • raspberry Pi


Dive into the research topics of 'Portable Low-Cost Fluorescence Reader for LFA Measurements'. Together they form a unique fingerprint.

Cite this