Portable Low-Cost Fluorescence Reader for LFA Measurements

Arttu Huttunen; Sanna Aikio; Marika Kurkinen; Jukka Tapani Mäkinen; Risto Mitikka; Liisa Kivimäki; Marja Harjumaa; Janne Takalo-Mattila; Christina Liedert; Jussi Hiltunen; Leena Hakalahti

doi:10.1109/JSEN.2020.2992894

Portable Low-Cost Fluorescence Reader for LFA Measurements

Arttu Huttunen, Sanna Aikio, Marika Kurkinen, Jukka Tapani Mäkinen, Risto Mitikka, Liisa Kivimäki, Marja Harjumaa, Janne Takalo-Mattila, Christina Liedert, Jussi Hiltunen, Leena Hakalahti

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

A portable device for measuring fluorescence signals from a lateral flow test strip was developed. The aim was to build an easy-to-use, affordable reader device for fast, quantitative analysis of point-of-care tests, made mostly of commercial off-the-shelf components. The device is capable of exciting fluorescent labels with 532 nm and 650 nm lasers and collecting the emitted fluorescent light with a camera. Core of the device is a Raspberry Pi Zero W wireless single board computer that controls the Raspberry Pi camera v2 module and lasers. The computer can also perform the image analysis and communicate the results to a mobile phone application acting as a user interface. Sensitivity of the device was characterized by sweeping the imaging parameters and comparing the performance with a high-grade fluorescence reader. Using dummy LFA samples, it was found that the device is able to detect signals from analyte concentrations that are relevant in biological samples. Image exposure time of 500~μ s was enough to read a distinguishable signal. The manufacturing costs were estimated to be below 100 euros per device in mass production.

Original language	English
Article number	9088161
Pages (from-to)	10275-10282
Journal	IEEE Sensors Journal
Volume	20
Issue number	17
DOIs	https://doi.org/10.1109/JSEN.2020.2992894
Publication status	Published - 1 Sept 2020
MoE publication type	A1 Journal article-refereed

Funding

This work was supported in part by the projects Home-CRP and the Printocent10 funded by Business Finland and European Regional Development Fund under Grant 3252/31/2015 and under Grant A73286 in part by the Academy of Finland Research Infrastructure Printed Intelligence Infrastructure (PII-FIRI) under Grant 320020, and in part by the Academy of Finland Flagship Programme, Photonics Research and Innovation (PREIN) decision under Grant 320168. The associate editor coordinating the review of this article and approving it for publication was Dr. Ying Zhang. (Corresponding author: Arttu Huttunen.) The authors are with the VTT Technical Research Centre of Finland Ltd., 90590 Oulu, Finland (e-mail: [email protected]). Digital Object Identifier 10.1109/JSEN.2020.2992894

Keywords

Biosensor
fluorescent label
image sensors
LFA
POC
raspberry Pi

Access to Document

10.1109/JSEN.2020.2992894

Cite this

@article{67e234f90a64439a935bd3d355bc12c7,

title = "Portable Low-Cost Fluorescence Reader for LFA Measurements",

abstract = "A portable device for measuring fluorescence signals from a lateral flow test strip was developed. The aim was to build an easy-to-use, affordable reader device for fast, quantitative analysis of point-of-care tests, made mostly of commercial off-the-shelf components. The device is capable of exciting fluorescent labels with 532 nm and 650 nm lasers and collecting the emitted fluorescent light with a camera. Core of the device is a Raspberry Pi Zero W wireless single board computer that controls the Raspberry Pi camera v2 module and lasers. The computer can also perform the image analysis and communicate the results to a mobile phone application acting as a user interface. Sensitivity of the device was characterized by sweeping the imaging parameters and comparing the performance with a high-grade fluorescence reader. Using dummy LFA samples, it was found that the device is able to detect signals from analyte concentrations that are relevant in biological samples. Image exposure time of 500~μ s was enough to read a distinguishable signal. The manufacturing costs were estimated to be below 100 euros per device in mass production.",

keywords = "Biosensor, fluorescent label, image sensors, LFA, POC, raspberry Pi",

author = "Arttu Huttunen and Sanna Aikio and Marika Kurkinen and M{\"a}kinen, {Jukka Tapani} and Risto Mitikka and Liisa Kivim{\"a}ki and Marja Harjumaa and Janne Takalo-Mattila and Christina Liedert and Jussi Hiltunen and Leena Hakalahti",

year = "2020",

month = sep,

day = "1",

doi = "10.1109/JSEN.2020.2992894",

language = "English",

volume = "20",

pages = "10275--10282",

journal = "IEEE Sensors Journal",

issn = "1530-437X",

publisher = "IEEE Institute of Electrical and Electronic Engineers",

number = "17",

}

TY - JOUR

T1 - Portable Low-Cost Fluorescence Reader for LFA Measurements

AU - Huttunen, Arttu

AU - Aikio, Sanna

AU - Kurkinen, Marika

AU - Mäkinen, Jukka Tapani

AU - Mitikka, Risto

AU - Kivimäki, Liisa

AU - Harjumaa, Marja

AU - Takalo-Mattila, Janne

AU - Liedert, Christina

AU - Hiltunen, Jussi

AU - Hakalahti, Leena

PY - 2020/9/1

Y1 - 2020/9/1

N2 - A portable device for measuring fluorescence signals from a lateral flow test strip was developed. The aim was to build an easy-to-use, affordable reader device for fast, quantitative analysis of point-of-care tests, made mostly of commercial off-the-shelf components. The device is capable of exciting fluorescent labels with 532 nm and 650 nm lasers and collecting the emitted fluorescent light with a camera. Core of the device is a Raspberry Pi Zero W wireless single board computer that controls the Raspberry Pi camera v2 module and lasers. The computer can also perform the image analysis and communicate the results to a mobile phone application acting as a user interface. Sensitivity of the device was characterized by sweeping the imaging parameters and comparing the performance with a high-grade fluorescence reader. Using dummy LFA samples, it was found that the device is able to detect signals from analyte concentrations that are relevant in biological samples. Image exposure time of 500~μ s was enough to read a distinguishable signal. The manufacturing costs were estimated to be below 100 euros per device in mass production.

AB - A portable device for measuring fluorescence signals from a lateral flow test strip was developed. The aim was to build an easy-to-use, affordable reader device for fast, quantitative analysis of point-of-care tests, made mostly of commercial off-the-shelf components. The device is capable of exciting fluorescent labels with 532 nm and 650 nm lasers and collecting the emitted fluorescent light with a camera. Core of the device is a Raspberry Pi Zero W wireless single board computer that controls the Raspberry Pi camera v2 module and lasers. The computer can also perform the image analysis and communicate the results to a mobile phone application acting as a user interface. Sensitivity of the device was characterized by sweeping the imaging parameters and comparing the performance with a high-grade fluorescence reader. Using dummy LFA samples, it was found that the device is able to detect signals from analyte concentrations that are relevant in biological samples. Image exposure time of 500~μ s was enough to read a distinguishable signal. The manufacturing costs were estimated to be below 100 euros per device in mass production.

KW - Biosensor

KW - fluorescent label

KW - image sensors

KW - LFA

KW - POC

KW - raspberry Pi

UR - http://www.scopus.com/inward/record.url?scp=85089501488&partnerID=8YFLogxK

U2 - 10.1109/JSEN.2020.2992894

DO - 10.1109/JSEN.2020.2992894

M3 - Article

AN - SCOPUS:85089501488

SN - 1530-437X

VL - 20

SP - 10275

EP - 10282

JO - IEEE Sensors Journal

JF - IEEE Sensors Journal

IS - 17

M1 - 9088161

ER -

Portable Low-Cost Fluorescence Reader for LFA Measurements

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this