Post operation inactivation of acidophilic bioleaching microorganisms using natural chloride-rich mine water

Malin Bomberg (Corresponding Author), Hanna Miettinen, Margareta Wahlström, Tommi Kaartinen, Sarita Ahoranta, Aino Maija Lakaniemi, Päivi Kinnunen

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

The H2020 BIOMOre project (www.biomore.info, Grant Agreement #642456) tests the feasibility of in situ bioleaching of copper in deep subsurface deposits in the Rudna mine, Poland. Copper is leached using biologically produced ferric iron solution, which is recycled back to the in situ reactor after re-oxidation by iron-oxidizing microorganisms. From a post operational point of view, it is important that the biological processes applied during the operation can be controlled and terminated. Our goal was to determine the possibility to use natural saline mine water for the inactivation of the introduced iron-oxidizing microorganisms remaining in the in situ reactor after completion of the leaching process of the ore block. Aerobic and anaerobic microcosms containing acid-leached (pH 2) sandstone or black shale from the Kupferschiefer in the Rudna mine were further leached with the effluent from a ferric iron generating bioreactor at 30 °C for 10 days to simulate the in situ leaching process. After the removal of the iron solution, residing iron-oxidizing microorganisms were inactivated by filling the microcosms with chloride-rich water (65 g L−1 Cl) originating from the mine. The chloride-rich water irreversibly inactivated the iron-oxidizing microorganisms and showed that the naturally occurring saline water of the mine can be used for long-term post process inactivation of bioleaching microorganisms

Original languageEnglish
Pages (from-to)236-245
Number of pages10
JournalHydrometallurgy
Volume180
DOIs
Publication statusPublished - 1 Sep 2018
MoE publication typeNot Eligible

Fingerprint

Bioleaching
Microorganisms
Chlorides
Iron
Water
Leaching
Copper
Saline water
Shale
Sandstone
Bioreactors
Ores
Effluents
Deposits
Oxidation
Acids

Keywords

  • BIOMOre
  • in situ bioleaching
  • Inactivation
  • Iron-oxidizing bacteria
  • Quantitative PCR

Cite this

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title = "Post operation inactivation of acidophilic bioleaching microorganisms using natural chloride-rich mine water",
abstract = "The H2020 BIOMOre project (www.biomore.info, Grant Agreement #642456) tests the feasibility of in situ bioleaching of copper in deep subsurface deposits in the Rudna mine, Poland. Copper is leached using biologically produced ferric iron solution, which is recycled back to the in situ reactor after re-oxidation by iron-oxidizing microorganisms. From a post operational point of view, it is important that the biological processes applied during the operation can be controlled and terminated. Our goal was to determine the possibility to use natural saline mine water for the inactivation of the introduced iron-oxidizing microorganisms remaining in the in situ reactor after completion of the leaching process of the ore block. Aerobic and anaerobic microcosms containing acid-leached (pH 2) sandstone or black shale from the Kupferschiefer in the Rudna mine were further leached with the effluent from a ferric iron generating bioreactor at 30 °C for 10 days to simulate the in situ leaching process. After the removal of the iron solution, residing iron-oxidizing microorganisms were inactivated by filling the microcosms with chloride-rich water (65 g L−1 Cl‐) originating from the mine. The chloride-rich water irreversibly inactivated the iron-oxidizing microorganisms and showed that the naturally occurring saline water of the mine can be used for long-term post process inactivation of bioleaching microorganisms",
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author = "Malin Bomberg and Hanna Miettinen and Margareta Wahlstr{\"o}m and Tommi Kaartinen and Sarita Ahoranta and Lakaniemi, {Aino Maija} and P{\"a}ivi Kinnunen",
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Post operation inactivation of acidophilic bioleaching microorganisms using natural chloride-rich mine water. / Bomberg, Malin (Corresponding Author); Miettinen, Hanna; Wahlström, Margareta; Kaartinen, Tommi; Ahoranta, Sarita; Lakaniemi, Aino Maija; Kinnunen, Päivi.

In: Hydrometallurgy, Vol. 180, 01.09.2018, p. 236-245.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Post operation inactivation of acidophilic bioleaching microorganisms using natural chloride-rich mine water

AU - Bomberg, Malin

AU - Miettinen, Hanna

AU - Wahlström, Margareta

AU - Kaartinen, Tommi

AU - Ahoranta, Sarita

AU - Lakaniemi, Aino Maija

AU - Kinnunen, Päivi

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N2 - The H2020 BIOMOre project (www.biomore.info, Grant Agreement #642456) tests the feasibility of in situ bioleaching of copper in deep subsurface deposits in the Rudna mine, Poland. Copper is leached using biologically produced ferric iron solution, which is recycled back to the in situ reactor after re-oxidation by iron-oxidizing microorganisms. From a post operational point of view, it is important that the biological processes applied during the operation can be controlled and terminated. Our goal was to determine the possibility to use natural saline mine water for the inactivation of the introduced iron-oxidizing microorganisms remaining in the in situ reactor after completion of the leaching process of the ore block. Aerobic and anaerobic microcosms containing acid-leached (pH 2) sandstone or black shale from the Kupferschiefer in the Rudna mine were further leached with the effluent from a ferric iron generating bioreactor at 30 °C for 10 days to simulate the in situ leaching process. After the removal of the iron solution, residing iron-oxidizing microorganisms were inactivated by filling the microcosms with chloride-rich water (65 g L−1 Cl‐) originating from the mine. The chloride-rich water irreversibly inactivated the iron-oxidizing microorganisms and showed that the naturally occurring saline water of the mine can be used for long-term post process inactivation of bioleaching microorganisms

AB - The H2020 BIOMOre project (www.biomore.info, Grant Agreement #642456) tests the feasibility of in situ bioleaching of copper in deep subsurface deposits in the Rudna mine, Poland. Copper is leached using biologically produced ferric iron solution, which is recycled back to the in situ reactor after re-oxidation by iron-oxidizing microorganisms. From a post operational point of view, it is important that the biological processes applied during the operation can be controlled and terminated. Our goal was to determine the possibility to use natural saline mine water for the inactivation of the introduced iron-oxidizing microorganisms remaining in the in situ reactor after completion of the leaching process of the ore block. Aerobic and anaerobic microcosms containing acid-leached (pH 2) sandstone or black shale from the Kupferschiefer in the Rudna mine were further leached with the effluent from a ferric iron generating bioreactor at 30 °C for 10 days to simulate the in situ leaching process. After the removal of the iron solution, residing iron-oxidizing microorganisms were inactivated by filling the microcosms with chloride-rich water (65 g L−1 Cl‐) originating from the mine. The chloride-rich water irreversibly inactivated the iron-oxidizing microorganisms and showed that the naturally occurring saline water of the mine can be used for long-term post process inactivation of bioleaching microorganisms

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