Abstract
This study sought to investigate the significance of raw materials
(starch-based glues, raw material papers) at different microbiologically
critical stages in the manufacturing process of refined paper products. The
study examined the occurrence of microorganisms in the process and in
end-product samples. Microbiological surveys verified that the production and
use of pasteurized starch-based glue was the most important factor
threatening the process hygiene and product safety. Subsequently, the
production and use of starch-based glue was changed, and a follow-up
programme targeting the microbiological quality of glue was developed as part
of a hygiene and safety management system. A total of 33 spore-forming
bacterial and 15 enterobacterial isolates were ribotyped, and 22 and 10
different ribogroups ( ribotypes), respectively, were generated. These
isolates from starch-based glue, raw material paper and end products were
atypical and, thus, in many cases physiological, chemotaxonomic (FAME) and
molecular ( partial 16S rDNA) results did not correspond. The most common
spore-forming bacteria (55% of the isolates) were Paenibacillus sp. and within
this genus several new species were also proposed. The most common
enterobacteria (87%) were Enterobacter cloacae and Citrobacter freundii
belonging to bacteria in hazard group 2, or species closely related to them.
It was demonstrated that the same spore-forming bacteria (ribotypes) were
present in both the glue samples and the end products (45% of isolates).
Original language | English |
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Pages (from-to) | 225-231 |
Journal | Journal of industrial microbiology and biotechnology |
Volume | 28 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2002 |
MoE publication type | A1 Journal article-refereed |
Keywords
- microbiology
- hygiene
- food-grade paper products
- starch-based glue
- RiboPrint