Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle

Eija Pöllänen (Corresponding Author), Vidal Fey, Timo Törmäkangas, Paula H. A. Ronkainen, Dennis R. Taaffe, Timo Takala, Satu Koskinen, Sulin Cheng, Jukka Puolakka, Urho M. Kujala, Harri Suominen, Sarianna Sipilä, Vuokko Kovanen

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

At the moment, there is no clear molecular explanation for the steeper decline in muscle performance after menopause or the mechanisms of counteractive treatments. The goal of this genome-wide study was to identify the genes and gene clusters through which power training (PT) comprising jumping activities or estrogen containing hormone replacement therapy (HRT) may affect skeletal muscle properties after menopause. We used musculus vastus lateralis samples from early stage postmenopausal (50–57 years old) women participating in a yearlong randomized double-blind placebo-controlled trial with PT and HRT interventions. Using microarray platform with over 24,000 probes, we identified 665 differentially expressed genes. The hierarchical clustering method was used to assort the genes. Additionally, enrichment analysis of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was carried out to clarify whether assorted gene clusters are enriched with particular functional categories. The analysis revealed transcriptional regulation of 49 GO/KEGG categories. PT upregulated transcription in “response to contraction”—category revealing novel candidate genes for contraction-related regulation of muscle function while HRT upregulated gene expression related to functionality of mitochondria. Moreover, several functional categories tightly related to muscle energy metabolism, development, and function were affected regardless of the treatment. Our results emphasize that during the early stages of the postmenopause, muscle properties are under transcriptional modulation, which both PT and HRT partially counteract leading to preservation of muscle power and potentially reducing the risk for aging-related muscle weakness. More specifically, PT and HRT may function through improving energy metabolism, response to contraction as well as by preserving functionality of the mitochondria.
Original languageEnglish
Pages (from-to)347-363
JournalAge
Volume32
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Multigene Family
Hormone Replacement Therapy
Skeletal Muscle
Hormones
Muscles
Encyclopedias
Genes
Gene Ontology
Genome
Menopause
Energy Metabolism
Mitochondria
Therapeutics
Postmenopause
Muscle Weakness
Quadriceps Muscle
Cluster Analysis
Estrogens
Placebos
Gene Expression

Keywords

  • Estrogen deprivation
  • Hormone replacement therapy
  • Menopause
  • Plyometric training
  • Power training
  • Skeletal muscle characteristics
  • Transcriptome-wide study

Cite this

Pöllänen, E., Fey, V., Törmäkangas, T., Ronkainen, P. H. A., Taaffe, D. R., Takala, T., ... Kovanen, V. (2010). Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle. Age, 32(3), 347-363. https://doi.org/10.1007/s11357-010-9140-1
Pöllänen, Eija ; Fey, Vidal ; Törmäkangas, Timo ; Ronkainen, Paula H. A. ; Taaffe, Dennis R. ; Takala, Timo ; Koskinen, Satu ; Cheng, Sulin ; Puolakka, Jukka ; Kujala, Urho M. ; Suominen, Harri ; Sipilä, Sarianna ; Kovanen, Vuokko. / Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle. In: Age. 2010 ; Vol. 32, No. 3. pp. 347-363.
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abstract = "At the moment, there is no clear molecular explanation for the steeper decline in muscle performance after menopause or the mechanisms of counteractive treatments. The goal of this genome-wide study was to identify the genes and gene clusters through which power training (PT) comprising jumping activities or estrogen containing hormone replacement therapy (HRT) may affect skeletal muscle properties after menopause. We used musculus vastus lateralis samples from early stage postmenopausal (50–57 years old) women participating in a yearlong randomized double-blind placebo-controlled trial with PT and HRT interventions. Using microarray platform with over 24,000 probes, we identified 665 differentially expressed genes. The hierarchical clustering method was used to assort the genes. Additionally, enrichment analysis of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was carried out to clarify whether assorted gene clusters are enriched with particular functional categories. The analysis revealed transcriptional regulation of 49 GO/KEGG categories. PT upregulated transcription in “response to contraction”—category revealing novel candidate genes for contraction-related regulation of muscle function while HRT upregulated gene expression related to functionality of mitochondria. Moreover, several functional categories tightly related to muscle energy metabolism, development, and function were affected regardless of the treatment. Our results emphasize that during the early stages of the postmenopause, muscle properties are under transcriptional modulation, which both PT and HRT partially counteract leading to preservation of muscle power and potentially reducing the risk for aging-related muscle weakness. More specifically, PT and HRT may function through improving energy metabolism, response to contraction as well as by preserving functionality of the mitochondria.",
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author = "Eija P{\"o}ll{\"a}nen and Vidal Fey and Timo T{\"o}rm{\"a}kangas and Ronkainen, {Paula H. A.} and Taaffe, {Dennis R.} and Timo Takala and Satu Koskinen and Sulin Cheng and Jukka Puolakka and Kujala, {Urho M.} and Harri Suominen and Sarianna Sipil{\"a} and Vuokko Kovanen",
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Pöllänen, E, Fey, V, Törmäkangas, T, Ronkainen, PHA, Taaffe, DR, Takala, T, Koskinen, S, Cheng, S, Puolakka, J, Kujala, UM, Suominen, H, Sipilä, S & Kovanen, V 2010, 'Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle', Age, vol. 32, no. 3, pp. 347-363. https://doi.org/10.1007/s11357-010-9140-1

Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle. / Pöllänen, Eija (Corresponding Author); Fey, Vidal; Törmäkangas, Timo; Ronkainen, Paula H. A.; Taaffe, Dennis R.; Takala, Timo; Koskinen, Satu; Cheng, Sulin; Puolakka, Jukka; Kujala, Urho M.; Suominen, Harri; Sipilä, Sarianna; Kovanen, Vuokko.

In: Age, Vol. 32, No. 3, 2010, p. 347-363.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle

AU - Pöllänen, Eija

AU - Fey, Vidal

AU - Törmäkangas, Timo

AU - Ronkainen, Paula H. A.

AU - Taaffe, Dennis R.

AU - Takala, Timo

AU - Koskinen, Satu

AU - Cheng, Sulin

AU - Puolakka, Jukka

AU - Kujala, Urho M.

AU - Suominen, Harri

AU - Sipilä, Sarianna

AU - Kovanen, Vuokko

PY - 2010

Y1 - 2010

N2 - At the moment, there is no clear molecular explanation for the steeper decline in muscle performance after menopause or the mechanisms of counteractive treatments. The goal of this genome-wide study was to identify the genes and gene clusters through which power training (PT) comprising jumping activities or estrogen containing hormone replacement therapy (HRT) may affect skeletal muscle properties after menopause. We used musculus vastus lateralis samples from early stage postmenopausal (50–57 years old) women participating in a yearlong randomized double-blind placebo-controlled trial with PT and HRT interventions. Using microarray platform with over 24,000 probes, we identified 665 differentially expressed genes. The hierarchical clustering method was used to assort the genes. Additionally, enrichment analysis of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was carried out to clarify whether assorted gene clusters are enriched with particular functional categories. The analysis revealed transcriptional regulation of 49 GO/KEGG categories. PT upregulated transcription in “response to contraction”—category revealing novel candidate genes for contraction-related regulation of muscle function while HRT upregulated gene expression related to functionality of mitochondria. Moreover, several functional categories tightly related to muscle energy metabolism, development, and function were affected regardless of the treatment. Our results emphasize that during the early stages of the postmenopause, muscle properties are under transcriptional modulation, which both PT and HRT partially counteract leading to preservation of muscle power and potentially reducing the risk for aging-related muscle weakness. More specifically, PT and HRT may function through improving energy metabolism, response to contraction as well as by preserving functionality of the mitochondria.

AB - At the moment, there is no clear molecular explanation for the steeper decline in muscle performance after menopause or the mechanisms of counteractive treatments. The goal of this genome-wide study was to identify the genes and gene clusters through which power training (PT) comprising jumping activities or estrogen containing hormone replacement therapy (HRT) may affect skeletal muscle properties after menopause. We used musculus vastus lateralis samples from early stage postmenopausal (50–57 years old) women participating in a yearlong randomized double-blind placebo-controlled trial with PT and HRT interventions. Using microarray platform with over 24,000 probes, we identified 665 differentially expressed genes. The hierarchical clustering method was used to assort the genes. Additionally, enrichment analysis of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was carried out to clarify whether assorted gene clusters are enriched with particular functional categories. The analysis revealed transcriptional regulation of 49 GO/KEGG categories. PT upregulated transcription in “response to contraction”—category revealing novel candidate genes for contraction-related regulation of muscle function while HRT upregulated gene expression related to functionality of mitochondria. Moreover, several functional categories tightly related to muscle energy metabolism, development, and function were affected regardless of the treatment. Our results emphasize that during the early stages of the postmenopause, muscle properties are under transcriptional modulation, which both PT and HRT partially counteract leading to preservation of muscle power and potentially reducing the risk for aging-related muscle weakness. More specifically, PT and HRT may function through improving energy metabolism, response to contraction as well as by preserving functionality of the mitochondria.

KW - Estrogen deprivation

KW - Hormone replacement therapy

KW - Menopause

KW - Plyometric training

KW - Power training

KW - Skeletal muscle characteristics

KW - Transcriptome-wide study

U2 - 10.1007/s11357-010-9140-1

DO - 10.1007/s11357-010-9140-1

M3 - Article

VL - 32

SP - 347

EP - 363

JO - GeroScience

JF - GeroScience

SN - 2509-2715

IS - 3

ER -