Pre-hydrolysis with carbohydrases facilitates the release of protein from brewer's spent grain

Piritta Niemi, D. Martins, Johanna Buchert, C.B. Faulds (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Brewer’s spent grain (BSG) is the most abundant side-stream from brewing. It is food-grade being rich in dietary fibre and protein and thus having potential as their source for both food and non-food applications. Initial treatment of milled BSG with a carbohydrase cocktail from Humicola insolens significantly enhanced the subsequent solubilisation of protein from the residual biomass. When treated with an alkaline protease, 76% of BSG protein was solubilized, whereas the yields were significantly lower with neutral or acidic proteases. In alkaline conditions significant amount of protein (53%) as predominantly low molecular weight protein was solubilized even without any protease addition. The degree of protein solubilisation was influenced by the time of exposure of modified BSG to the alkaline environment. The non-enzymatic protein solubilisation was, however, only observed when BSG had been initially treated with the carbohydrase, suggesting the protein is surrounded by cell wall polysaccharides restricting its initial release.
Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalBioresource Technology
Volume136
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

hydrolysis
Hydrolysis
Proteins
protein
solubilization
Peptide Hydrolases
Brewing
alkaline environment
food
carbohydrase
Polysaccharides
polysaccharide
Biomass
Molecular weight
Cells
biomass

Keywords

  • brewer's spent grain
  • carbohydrate removal
  • enzymatic solubilisation
  • protease
  • protein

Cite this

Niemi, Piritta ; Martins, D. ; Buchert, Johanna ; Faulds, C.B. / Pre-hydrolysis with carbohydrases facilitates the release of protein from brewer's spent grain. In: Bioresource Technology. 2013 ; Vol. 136. pp. 529-534.
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Pre-hydrolysis with carbohydrases facilitates the release of protein from brewer's spent grain. / Niemi, Piritta; Martins, D.; Buchert, Johanna; Faulds, C.B. (Corresponding Author).

In: Bioresource Technology, Vol. 136, 2013, p. 529-534.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Pre-hydrolysis with carbohydrases facilitates the release of protein from brewer's spent grain

AU - Niemi, Piritta

AU - Martins, D.

AU - Buchert, Johanna

AU - Faulds, C.B.

PY - 2013

Y1 - 2013

N2 - Brewer’s spent grain (BSG) is the most abundant side-stream from brewing. It is food-grade being rich in dietary fibre and protein and thus having potential as their source for both food and non-food applications. Initial treatment of milled BSG with a carbohydrase cocktail from Humicola insolens significantly enhanced the subsequent solubilisation of protein from the residual biomass. When treated with an alkaline protease, 76% of BSG protein was solubilized, whereas the yields were significantly lower with neutral or acidic proteases. In alkaline conditions significant amount of protein (53%) as predominantly low molecular weight protein was solubilized even without any protease addition. The degree of protein solubilisation was influenced by the time of exposure of modified BSG to the alkaline environment. The non-enzymatic protein solubilisation was, however, only observed when BSG had been initially treated with the carbohydrase, suggesting the protein is surrounded by cell wall polysaccharides restricting its initial release.

AB - Brewer’s spent grain (BSG) is the most abundant side-stream from brewing. It is food-grade being rich in dietary fibre and protein and thus having potential as their source for both food and non-food applications. Initial treatment of milled BSG with a carbohydrase cocktail from Humicola insolens significantly enhanced the subsequent solubilisation of protein from the residual biomass. When treated with an alkaline protease, 76% of BSG protein was solubilized, whereas the yields were significantly lower with neutral or acidic proteases. In alkaline conditions significant amount of protein (53%) as predominantly low molecular weight protein was solubilized even without any protease addition. The degree of protein solubilisation was influenced by the time of exposure of modified BSG to the alkaline environment. The non-enzymatic protein solubilisation was, however, only observed when BSG had been initially treated with the carbohydrase, suggesting the protein is surrounded by cell wall polysaccharides restricting its initial release.

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KW - enzymatic solubilisation

KW - protease

KW - protein

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JF - Bioresource Technology

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