Preferential synthesis of heptaasyl lipopolysaccharide by the ssc permeability mutant of Salmonella typhimurium

Ilkka Helander, Laura Hirvas, Jari Tuominen, Martti Vaara

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

In Salmonella typhimurium, a chromosomal gene termed ssc has been shown to cause an antibiotic‐supersensitive phenotype. We studied the effect of the ssc gene on the chemical composition of the lipopolysaccharide component, using a thermosensitive ssc1 mutant (SH7622) that grows poorly at 42°C. Analysis of the lipopolysaccharide by various techniques including fast‐atom‐bombardment mass spectrometry of lipid A, and determination of the type of linkage of fatty acids, revealed a profound temperature‐dependent effect associated with the ssc1 mutation. At the non‐permissive temperature, SH7622 contained hexadecanoic acid in the majority of lipid A molecules, resulting in the exclusive presence of heptaacyl lipopolysaccharide. This effect was largely reversed by the introduction of the cloned wild‐type ssc gene to SH7622 and much reduced by growth of SH7622 at 37°C.
Original languageEnglish
Pages (from-to)1101-1106
Number of pages6
JournalEuropean Journal of Biochemistry
Volume204
Issue number3
DOIs
Publication statusPublished - 1992
MoE publication typeA1 Journal article-refereed

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Salmonella
Salmonella typhimurium
Lipopolysaccharides
Permeability
Lipid A
Genes
Palmitic Acid
Mass spectrometry
Mass Spectrometry
Fatty Acids
Phenotype
Mutation
Molecules
Temperature
Growth
Chemical analysis

Cite this

Helander, Ilkka ; Hirvas, Laura ; Tuominen, Jari ; Vaara, Martti. / Preferential synthesis of heptaasyl lipopolysaccharide by the ssc permeability mutant of Salmonella typhimurium. In: European Journal of Biochemistry. 1992 ; Vol. 204, No. 3. pp. 1101-1106.
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Preferential synthesis of heptaasyl lipopolysaccharide by the ssc permeability mutant of Salmonella typhimurium. / Helander, Ilkka; Hirvas, Laura; Tuominen, Jari; Vaara, Martti.

In: European Journal of Biochemistry, Vol. 204, No. 3, 1992, p. 1101-1106.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Preferential synthesis of heptaasyl lipopolysaccharide by the ssc permeability mutant of Salmonella typhimurium

AU - Helander, Ilkka

AU - Hirvas, Laura

AU - Tuominen, Jari

AU - Vaara, Martti

PY - 1992

Y1 - 1992

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AB - In Salmonella typhimurium, a chromosomal gene termed ssc has been shown to cause an antibiotic‐supersensitive phenotype. We studied the effect of the ssc gene on the chemical composition of the lipopolysaccharide component, using a thermosensitive ssc1 mutant (SH7622) that grows poorly at 42°C. Analysis of the lipopolysaccharide by various techniques including fast‐atom‐bombardment mass spectrometry of lipid A, and determination of the type of linkage of fatty acids, revealed a profound temperature‐dependent effect associated with the ssc1 mutation. At the non‐permissive temperature, SH7622 contained hexadecanoic acid in the majority of lipid A molecules, resulting in the exclusive presence of heptaacyl lipopolysaccharide. This effect was largely reversed by the introduction of the cloned wild‐type ssc gene to SH7622 and much reduced by growth of SH7622 at 37°C.

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