Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 625-633 |
| Number of pages | 9 |
| Journal | Journal of Medical Microbiology |
| Volume | 55 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- irritable bowel syndrome
- IBS
- clostridial microbiota
- faecal
- faecal microbiota
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Prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria. / Maukonen, Johanna (Corresponding Author); Satokari, Reetta; Mättö, Jaana; Söderlund, Hans; Mattila-Sandholm, Tiina; Saarela, Maria.
In: Journal of Medical Microbiology, Vol. 55, No. 5, 2006, p. 625-633.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria
AU - Maukonen, Johanna
AU - Satokari, Reetta
AU - Mättö, Jaana
AU - Söderlund, Hans
AU - Mattila-Sandholm, Tiina
AU - Saarela, Maria
PY - 2006
Y1 - 2006
N2 - The differences in faecal bacterial population between irritable bowel syndrome (IBS) and control subjects have been reported in several studies. The aim of the present study was to compare the predominant and clostridial faecal microbiota of IBS subjects and healthy controls by applying denaturing gradient gel electrophoresis (DGGE) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (TRAC). According to the results, the studied clostridial groups (Clostridium histolyticum, Clostridium coccoides-Eubacterium rectale, Clostridium lituseburense and Clostridium leptum) represented the dominant faecal microbiota of most of the studied subjects, comprising altogether 29–87 % of the total bacteria as determined by the hybridized 16S rRNA. The C. coccoides-E. rectale group was the dominant subgroup of clostridia, contributing a mean of 43 % of the total bacteria in control subjects and 30 % (constipation type) to 50 % (diarrhoea type) in different IBS symptom category subjects. The proportion of the C. coccoides-E. rectale group was found to be significantly lower in the constipation-type IBS subjects than in the control subjects. DNA-based PCR-DGGE and RNA-based RT-PCR-DGGE analyses targeted to the predominant bacterial population showed considerable biodiversity as well as uniqueness of the microbiota in each subject, in both control and IBS subject groups. The RT-PCR-DGGE profiles of the IBS subjects further indicated higher instability of the bacterial population compared to the control subjects. The observations suggest that clostridial microbiota, in addition to the instability of the active predominant faecal bacterial population, may be involved in IBS.
AB - The differences in faecal bacterial population between irritable bowel syndrome (IBS) and control subjects have been reported in several studies. The aim of the present study was to compare the predominant and clostridial faecal microbiota of IBS subjects and healthy controls by applying denaturing gradient gel electrophoresis (DGGE) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (TRAC). According to the results, the studied clostridial groups (Clostridium histolyticum, Clostridium coccoides-Eubacterium rectale, Clostridium lituseburense and Clostridium leptum) represented the dominant faecal microbiota of most of the studied subjects, comprising altogether 29–87 % of the total bacteria as determined by the hybridized 16S rRNA. The C. coccoides-E. rectale group was the dominant subgroup of clostridia, contributing a mean of 43 % of the total bacteria in control subjects and 30 % (constipation type) to 50 % (diarrhoea type) in different IBS symptom category subjects. The proportion of the C. coccoides-E. rectale group was found to be significantly lower in the constipation-type IBS subjects than in the control subjects. DNA-based PCR-DGGE and RNA-based RT-PCR-DGGE analyses targeted to the predominant bacterial population showed considerable biodiversity as well as uniqueness of the microbiota in each subject, in both control and IBS subject groups. The RT-PCR-DGGE profiles of the IBS subjects further indicated higher instability of the bacterial population compared to the control subjects. The observations suggest that clostridial microbiota, in addition to the instability of the active predominant faecal bacterial population, may be involved in IBS.
KW - irritable bowel syndrome
KW - IBS
KW - clostridial microbiota
KW - faecal
KW - faecal microbiota
U2 - 10.1099/jmm.0.46134-0
DO - 10.1099/jmm.0.46134-0
M3 - Article
VL - 55
SP - 625
EP - 633
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
SN - 0022-2615
IS - 5
ER -