Priming effect as determined by adding 14C-glucose to modified controlled composting test

Marja Tuomela (Corresponding Author), Annele Hatakka, Sari Karjomaa, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

The development of new biodegradable packaging materials, especially biodegradable plastics, has created a need for biodegradability testing.
The European standard for controlled composting test was used in this study for assessing if the addition of a test material results in excess CO2 production in compost. This effect, designated as the priming effect, would give an erroneous result for biodegradation, which is based on CO2 formation from the test material.
Glucose was selected as a test substrate because it is the degradation product of starch and cellulose, which are major compounds of many packaging materials. Both 14C-glucose and non-labelled glucose was applied to nine compost samples of variable stability and agefrom two weeks to 1.5 years. CO2 and 14CO2 evolution were measured during the incubation.
Biodegradation of glucose in unstable composts (age leq6 months) was negative and 14CO2 evolution was poor, although the respective composts without glucose produced relatively high amounts of CO2.
It was concluded that a negative priming effect was observed in unstable composts, in which glucose remained mostly non-degraded and apparently inhibited the mineralization of native organic matter in the compost. In stable composts (age ≥6 months), biodegradation of glucose was high and approximately equal to 14C-glucose mineralization, i.e., the composts showed no priming effect.
Young composts were unsuitable for controlled composting test due to lack of stability. It is important to ensure that the compost inoculum used for the test is sufficiently stable.
Original languageEnglish
Pages (from-to)131-140
JournalBiodegradation
Volume13
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Composting
composting
compost
Glucose
glucose
Soil
Biodegradation
Packaging materials
biodegradation
Product Packaging
Biodegradable Plastics
test
effect
Biodegradability
mineralization
Starch
Cellulose
Biological materials
starch
cellulose

Keywords

  • biodegradation
  • compost
  • compost maturity
  • glucose
  • priming effect

Cite this

Tuomela, Marja ; Hatakka, Annele ; Karjomaa, Sari ; Itävaara, Merja. / Priming effect as determined by adding 14C-glucose to modified controlled composting test. In: Biodegradation. 2002 ; Vol. 13, No. 2. pp. 131-140.
@article{145d35218fd14576a0b668bc156c3216,
title = "Priming effect as determined by adding 14C-glucose to modified controlled composting test",
abstract = "The development of new biodegradable packaging materials, especially biodegradable plastics, has created a need for biodegradability testing. The European standard for controlled composting test was used in this study for assessing if the addition of a test material results in excess CO2 production in compost. This effect, designated as the priming effect, would give an erroneous result for biodegradation, which is based on CO2 formation from the test material. Glucose was selected as a test substrate because it is the degradation product of starch and cellulose, which are major compounds of many packaging materials. Both 14C-glucose and non-labelled glucose was applied to nine compost samples of variable stability and agefrom two weeks to 1.5 years. CO2 and 14CO2 evolution were measured during the incubation. Biodegradation of glucose in unstable composts (age leq6 months) was negative and 14CO2 evolution was poor, although the respective composts without glucose produced relatively high amounts of CO2. It was concluded that a negative priming effect was observed in unstable composts, in which glucose remained mostly non-degraded and apparently inhibited the mineralization of native organic matter in the compost. In stable composts (age ≥6 months), biodegradation of glucose was high and approximately equal to 14C-glucose mineralization, i.e., the composts showed no priming effect. Young composts were unsuitable for controlled composting test due to lack of stability. It is important to ensure that the compost inoculum used for the test is sufficiently stable.",
keywords = "biodegradation, compost, compost maturity, glucose, priming effect",
author = "Marja Tuomela and Annele Hatakka and Sari Karjomaa and Merja It{\"a}vaara",
year = "2002",
doi = "10.1023/A:1020498209",
language = "English",
volume = "13",
pages = "131--140",
journal = "Biodegradation",
issn = "0923-9820",
publisher = "Springer",
number = "2",

}

Priming effect as determined by adding 14C-glucose to modified controlled composting test. / Tuomela, Marja (Corresponding Author); Hatakka, Annele; Karjomaa, Sari; Itävaara, Merja.

In: Biodegradation, Vol. 13, No. 2, 2002, p. 131-140.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Priming effect as determined by adding 14C-glucose to modified controlled composting test

AU - Tuomela, Marja

AU - Hatakka, Annele

AU - Karjomaa, Sari

AU - Itävaara, Merja

PY - 2002

Y1 - 2002

N2 - The development of new biodegradable packaging materials, especially biodegradable plastics, has created a need for biodegradability testing. The European standard for controlled composting test was used in this study for assessing if the addition of a test material results in excess CO2 production in compost. This effect, designated as the priming effect, would give an erroneous result for biodegradation, which is based on CO2 formation from the test material. Glucose was selected as a test substrate because it is the degradation product of starch and cellulose, which are major compounds of many packaging materials. Both 14C-glucose and non-labelled glucose was applied to nine compost samples of variable stability and agefrom two weeks to 1.5 years. CO2 and 14CO2 evolution were measured during the incubation. Biodegradation of glucose in unstable composts (age leq6 months) was negative and 14CO2 evolution was poor, although the respective composts without glucose produced relatively high amounts of CO2. It was concluded that a negative priming effect was observed in unstable composts, in which glucose remained mostly non-degraded and apparently inhibited the mineralization of native organic matter in the compost. In stable composts (age ≥6 months), biodegradation of glucose was high and approximately equal to 14C-glucose mineralization, i.e., the composts showed no priming effect. Young composts were unsuitable for controlled composting test due to lack of stability. It is important to ensure that the compost inoculum used for the test is sufficiently stable.

AB - The development of new biodegradable packaging materials, especially biodegradable plastics, has created a need for biodegradability testing. The European standard for controlled composting test was used in this study for assessing if the addition of a test material results in excess CO2 production in compost. This effect, designated as the priming effect, would give an erroneous result for biodegradation, which is based on CO2 formation from the test material. Glucose was selected as a test substrate because it is the degradation product of starch and cellulose, which are major compounds of many packaging materials. Both 14C-glucose and non-labelled glucose was applied to nine compost samples of variable stability and agefrom two weeks to 1.5 years. CO2 and 14CO2 evolution were measured during the incubation. Biodegradation of glucose in unstable composts (age leq6 months) was negative and 14CO2 evolution was poor, although the respective composts without glucose produced relatively high amounts of CO2. It was concluded that a negative priming effect was observed in unstable composts, in which glucose remained mostly non-degraded and apparently inhibited the mineralization of native organic matter in the compost. In stable composts (age ≥6 months), biodegradation of glucose was high and approximately equal to 14C-glucose mineralization, i.e., the composts showed no priming effect. Young composts were unsuitable for controlled composting test due to lack of stability. It is important to ensure that the compost inoculum used for the test is sufficiently stable.

KW - biodegradation

KW - compost

KW - compost maturity

KW - glucose

KW - priming effect

U2 - 10.1023/A:1020498209

DO - 10.1023/A:1020498209

M3 - Article

VL - 13

SP - 131

EP - 140

JO - Biodegradation

JF - Biodegradation

SN - 0923-9820

IS - 2

ER -