Printable and flexible macroporous organosilica film with high protein adsorption capacity

J.J. Heikkinen, Liisa Kivimäki, V.P. Hytönen, M.S. Kulomaa, O.E.O. Hormi (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

An approach for creating a flexible and macroporous silsesquioxane film using phase separation method is described. The porous film was prepared by a simple coating method where sol–gel solution containing polyacrylic acid (PAA) and 1,6-bis(trimethoxysilyl)hexane in water was applied on boehmite silica coated polymethylmethacrylate (PMMA) film. After drying, the water soluble PAA template was removed by washing the film with water revealing the porous film. With certain ratios of PAA and water, fully co-continuous pore system with open surface was obtained. Porous films with 3–4 μm thickness were found to be highly flexible. The biocompatibility of the porous film was tested by immobilizing a high affinity biotin-binding chimeric avidin (ChiAVD(I117Y)) into the porous matrix The porous film was found to adsorb higher amounts of functional chimeric avidin compared to the pure PMMA film or a boehmite silica coated PMMA film.

Original languageEnglish
Pages (from-to)1934-1937
Number of pages4
JournalThin Solid Films
Volume520
Issue number6
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

carbopol 940
proteins
Proteins
Adsorption
adsorption
Polymethyl Methacrylate
Avidin
Water
Silicon Dioxide
water
acids
Acids
Silica
silicon dioxide
biotin
washing
Hexanes
biocompatibility
Biotin
Hexane

Keywords

  • Flexible coatings
  • phase separation
  • porous films
  • printable coatings
  • protein immobilization
  • sol-gel

Cite this

Heikkinen, J.J. ; Kivimäki, Liisa ; Hytönen, V.P. ; Kulomaa, M.S. ; Hormi, O.E.O. / Printable and flexible macroporous organosilica film with high protein adsorption capacity. In: Thin Solid Films. 2012 ; Vol. 520, No. 6. pp. 1934-1937.
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abstract = "An approach for creating a flexible and macroporous silsesquioxane film using phase separation method is described. The porous film was prepared by a simple coating method where sol–gel solution containing polyacrylic acid (PAA) and 1,6-bis(trimethoxysilyl)hexane in water was applied on boehmite silica coated polymethylmethacrylate (PMMA) film. After drying, the water soluble PAA template was removed by washing the film with water revealing the porous film. With certain ratios of PAA and water, fully co-continuous pore system with open surface was obtained. Porous films with 3–4 μm thickness were found to be highly flexible. The biocompatibility of the porous film was tested by immobilizing a high affinity biotin-binding chimeric avidin (ChiAVD(I117Y)) into the porous matrix The porous film was found to adsorb higher amounts of functional chimeric avidin compared to the pure PMMA film or a boehmite silica coated PMMA film.",
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Printable and flexible macroporous organosilica film with high protein adsorption capacity. / Heikkinen, J.J.; Kivimäki, Liisa; Hytönen, V.P.; Kulomaa, M.S.; Hormi, O.E.O. (Corresponding Author).

In: Thin Solid Films, Vol. 520, No. 6, 2012, p. 1934-1937.

Research output: Contribution to journalArticleScientificpeer-review

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AB - An approach for creating a flexible and macroporous silsesquioxane film using phase separation method is described. The porous film was prepared by a simple coating method where sol–gel solution containing polyacrylic acid (PAA) and 1,6-bis(trimethoxysilyl)hexane in water was applied on boehmite silica coated polymethylmethacrylate (PMMA) film. After drying, the water soluble PAA template was removed by washing the film with water revealing the porous film. With certain ratios of PAA and water, fully co-continuous pore system with open surface was obtained. Porous films with 3–4 μm thickness were found to be highly flexible. The biocompatibility of the porous film was tested by immobilizing a high affinity biotin-binding chimeric avidin (ChiAVD(I117Y)) into the porous matrix The porous film was found to adsorb higher amounts of functional chimeric avidin compared to the pure PMMA film or a boehmite silica coated PMMA film.

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