Abstract
Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor
(TvL) were printed onto paper substrate by three different methods:
screen printing, rod coating, and flexo printing. Microcapsules were
fabricated via interfacial polycondensation of PEI with the cross-linker
sebacoyl chloride, incorporated into an ink, and printed or coated on
the paper substrate. The same ink components were used for three
printing methods, and it was found that laccase microcapsules were
compatible with the ink. Enzymatic activity of microencapsulated TvL was
maintained constant in polymer-based ink for at least eight weeks.
Thick layers with high enzymatic activity were obtained when
laccase-containing microcapsules were screen printed on paper substrate.
Flexo printed bioactive paper showed very low activity, since by using
this printing method the paper surface was not fully covered by enzyme
microcapsules. Finally, screen printing provided a bioactive paper with
high water-resistance and the highest enzyme lifetime.
Original language | English |
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Pages (from-to) | 2008-2015 |
Journal | Biomacromolecules |
Volume | 12 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2011 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Biotechnology
- Biotechnology: methods
- Capsules
- Capsules: chemistry
- Capsules: metabolism
- Drug Compounding
- Drug Compounding: methods
- Enzymes
- Fungal Proteins
- Fungal Proteins: chemistry
- Fungal Proteins: metabolism
- Immobilized
- Immobilized: chemistry
- Immobilized: metabolism
- Ink
- Laccase
- Laccase: chemistry
- Laccase: metabolism
- Lignin
- Lignin: metabolism
- Paper
- Polyethyleneimine
- Polyethyleneimine: chemistry
- Polyethyleneimine: metabolism
- Printing
- Printing: methods
- Trametes
- Trametes: chemistry
- Trametes: enzymology