Printing of polymer microcapsules for enzyme immobilization on paper substrate

Anne Savolainen, Yufen Zhang, Dominic Rochefort, Ulla Holopainen, Tomi Erho, Jouko Virtanen, Maria Smolander (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)


Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.
Original languageEnglish
Pages (from-to)2008-2015
Number of pages8
Issue number6
Publication statusPublished - Jun 2011
MoE publication typeA1 Journal article-refereed


  • Biotechnology
  • Biotechnology: methods
  • Capsules
  • Capsules: chemistry
  • Capsules: metabolism
  • Drug Compounding
  • Drug Compounding: methods
  • Enzymes
  • Fungal Proteins
  • Fungal Proteins: chemistry
  • Fungal Proteins: metabolism
  • Immobilized
  • Immobilized: chemistry
  • Immobilized: metabolism
  • Ink
  • Laccase
  • Laccase: chemistry
  • Laccase: metabolism
  • Lignin
  • Lignin: metabolism
  • Paper
  • Polyethyleneimine
  • Polyethyleneimine: chemistry
  • Polyethyleneimine: metabolism
  • Printing
  • Printing: methods
  • Trametes
  • Trametes: chemistry
  • Trametes: enzymology

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