Printing of polymer microcapsules for enzyme immobilization on paper substrate

Anne Savolainen, Yufen Zhang, Dominic Rochefort, Ulla Holopainen, Tomi Erho, Jouko Virtanen, Maria Smolander (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.
Original languageEnglish
Pages (from-to)2008-2015
Number of pages8
JournalBiomacromolecules
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzyme immobilization
Ink
Capsules
Printing
Polymers
Laccase
Polyetherimides
Screen printing
Substrates
Enzymes
Interfacial polycondensation
Coatings
Water

Keywords

  • Biotechnology
  • Biotechnology: methods
  • Capsules
  • Capsules: chemistry
  • Capsules: metabolism
  • Drug Compounding
  • Drug Compounding: methods
  • Enzymes
  • Fungal Proteins
  • Fungal Proteins: chemistry
  • Fungal Proteins: metabolism
  • Immobilized
  • Immobilized: chemistry
  • Immobilized: metabolism
  • Ink
  • Laccase
  • Laccase: chemistry
  • Laccase: metabolism
  • Lignin
  • Lignin: metabolism
  • Paper
  • Polyethyleneimine
  • Polyethyleneimine: chemistry
  • Polyethyleneimine: metabolism
  • Printing
  • Printing: methods
  • Trametes
  • Trametes: chemistry
  • Trametes: enzymology

Cite this

Savolainen, Anne ; Zhang, Yufen ; Rochefort, Dominic ; Holopainen, Ulla ; Erho, Tomi ; Virtanen, Jouko ; Smolander, Maria. / Printing of polymer microcapsules for enzyme immobilization on paper substrate. In: Biomacromolecules. 2011 ; Vol. 12, No. 6. pp. 2008-2015.
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abstract = "Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.",
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author = "Anne Savolainen and Yufen Zhang and Dominic Rochefort and Ulla Holopainen and Tomi Erho and Jouko Virtanen and Maria Smolander",
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volume = "12",
pages = "2008--2015",
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Printing of polymer microcapsules for enzyme immobilization on paper substrate. / Savolainen, Anne; Zhang, Yufen; Rochefort, Dominic; Holopainen, Ulla; Erho, Tomi; Virtanen, Jouko; Smolander, Maria (Corresponding Author).

In: Biomacromolecules, Vol. 12, No. 6, 06.2011, p. 2008-2015.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Printing of polymer microcapsules for enzyme immobilization on paper substrate

AU - Savolainen, Anne

AU - Zhang, Yufen

AU - Rochefort, Dominic

AU - Holopainen, Ulla

AU - Erho, Tomi

AU - Virtanen, Jouko

AU - Smolander, Maria

PY - 2011/6

Y1 - 2011/6

N2 - Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.

AB - Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.

KW - Biotechnology

KW - Biotechnology: methods

KW - Capsules

KW - Capsules: chemistry

KW - Capsules: metabolism

KW - Drug Compounding

KW - Drug Compounding: methods

KW - Enzymes

KW - Fungal Proteins

KW - Fungal Proteins: chemistry

KW - Fungal Proteins: metabolism

KW - Immobilized

KW - Immobilized: chemistry

KW - Immobilized: metabolism

KW - Ink

KW - Laccase

KW - Laccase: chemistry

KW - Laccase: metabolism

KW - Lignin

KW - Lignin: metabolism

KW - Paper

KW - Polyethyleneimine

KW - Polyethyleneimine: chemistry

KW - Polyethyleneimine: metabolism

KW - Printing

KW - Printing: methods

KW - Trametes

KW - Trametes: chemistry

KW - Trametes: enzymology

U2 - 10.1021/bm2003434

DO - 10.1021/bm2003434

M3 - Article

VL - 12

SP - 2008

EP - 2015

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 6

ER -