Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2008-2015 |
| Number of pages | 8 |
| Journal | Biomacromolecules |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2011 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Biotechnology
- Biotechnology: methods
- Capsules
- Capsules: chemistry
- Capsules: metabolism
- Drug Compounding
- Drug Compounding: methods
- Enzymes
- Fungal Proteins
- Fungal Proteins: chemistry
- Fungal Proteins: metabolism
- Immobilized
- Immobilized: chemistry
- Immobilized: metabolism
- Ink
- Laccase
- Laccase: chemistry
- Laccase: metabolism
- Lignin
- Lignin: metabolism
- Paper
- Polyethyleneimine
- Polyethyleneimine: chemistry
- Polyethyleneimine: metabolism
- Printing
- Printing: methods
- Trametes
- Trametes: chemistry
- Trametes: enzymology
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Printing of polymer microcapsules for enzyme immobilization on paper substrate. / Savolainen, Anne; Zhang, Yufen; Rochefort, Dominic; Holopainen, Ulla; Erho, Tomi; Virtanen, Jouko; Smolander, Maria (Corresponding Author).
In: Biomacromolecules, Vol. 12, No. 6, 06.2011, p. 2008-2015.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Printing of polymer microcapsules for enzyme immobilization on paper substrate
AU - Savolainen, Anne
AU - Zhang, Yufen
AU - Rochefort, Dominic
AU - Holopainen, Ulla
AU - Erho, Tomi
AU - Virtanen, Jouko
AU - Smolander, Maria
PY - 2011/6
Y1 - 2011/6
N2 - Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.
AB - Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.
KW - Biotechnology
KW - Biotechnology: methods
KW - Capsules
KW - Capsules: chemistry
KW - Capsules: metabolism
KW - Drug Compounding
KW - Drug Compounding: methods
KW - Enzymes
KW - Fungal Proteins
KW - Fungal Proteins: chemistry
KW - Fungal Proteins: metabolism
KW - Immobilized
KW - Immobilized: chemistry
KW - Immobilized: metabolism
KW - Ink
KW - Laccase
KW - Laccase: chemistry
KW - Laccase: metabolism
KW - Lignin
KW - Lignin: metabolism
KW - Paper
KW - Polyethyleneimine
KW - Polyethyleneimine: chemistry
KW - Polyethyleneimine: metabolism
KW - Printing
KW - Printing: methods
KW - Trametes
KW - Trametes: chemistry
KW - Trametes: enzymology
U2 - 10.1021/bm2003434
DO - 10.1021/bm2003434
M3 - Article
VL - 12
SP - 2008
EP - 2015
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 6
ER -