Probability of growth and toxin production by nonproteolytic Clostridium botulinum in rockfish stored under modified atmospheres

Seppo Lindroth, Constantin Genigeorgis

Research output: Contribution to journalArticleScientificpeer-review

63 Citations (Scopus)

Abstract

The potential risk of C. botulinum growth in fresh fish stored under modified atmospheres remains unclear. Few studies have identified qualitatively certain conditions leading to toxigenesis. This paper is the First of a series attempting to quantify the effect of a variety of parameters on the probability (P) of toxigenesis by one spore in fish. The factorial design experiments included red snapper tissue homogenate inoculated with a pool of nonproteolytic spores (5 type E, 4 type B and 4 type F strains) at 7 levels (104−10−2 per 3 g sample) and incubated at 4, 8, 12, 17 and 30°C under 3 modified atmospheres (vacuum, 100% CO2 and 70% CO2+30% air) for up to 21 days. At the 100 spore/sample level the earliest time to detect toxin production at 4, 8, 12, 17 and 30°C under all modified atmospheres was > 21, 12, 9, 6 and 2 days, respectively. At the 101 spores/sample level the earliest times for the same temperatures were > 21, 9, 6, 3–6 and 1–2 days, respectively. The probability of toxigenesis was affected significantly (P < 0.005) by temperature, storage time, atmosphere×temperature, and temperature×time but not by atmosphere (P > 0.1). Using linear and logistic regression models, equations were derived which can predict the P of 1 spore initiating growth and toxigenesis by a particular day and at a particular temperature of storage. Studies involving other fish substrates are in progress.

Original languageEnglish
Pages (from-to)167 - 181
Number of pages15
JournalInternational Journal of Food Microbiology
Volume3
Issue number3
DOIs
Publication statusPublished - 1986
MoE publication typeNot Eligible

Fingerprint

Clostridium botulinum
toxigenesis
rockfish
Spores
Atmosphere
toxins
spores
Growth
Fishes
Logistic Models
fish
carbon dioxide
Temperature
snapper
Vacuum
sampling
storage temperature
Linear Models
experimental design
Air

Cite this

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title = "Probability of growth and toxin production by nonproteolytic Clostridium botulinum in rockfish stored under modified atmospheres",
abstract = "The potential risk of C. botulinum growth in fresh fish stored under modified atmospheres remains unclear. Few studies have identified qualitatively certain conditions leading to toxigenesis. This paper is the First of a series attempting to quantify the effect of a variety of parameters on the probability (P) of toxigenesis by one spore in fish. The factorial design experiments included red snapper tissue homogenate inoculated with a pool of nonproteolytic spores (5 type E, 4 type B and 4 type F strains) at 7 levels (104−10−2 per 3 g sample) and incubated at 4, 8, 12, 17 and 30°C under 3 modified atmospheres (vacuum, 100{\%} CO2 and 70{\%} CO2+30{\%} air) for up to 21 days. At the 100 spore/sample level the earliest time to detect toxin production at 4, 8, 12, 17 and 30°C under all modified atmospheres was > 21, 12, 9, 6 and 2 days, respectively. At the 101 spores/sample level the earliest times for the same temperatures were > 21, 9, 6, 3–6 and 1–2 days, respectively. The probability of toxigenesis was affected significantly (P < 0.005) by temperature, storage time, atmosphere×temperature, and temperature×time but not by atmosphere (P > 0.1). Using linear and logistic regression models, equations were derived which can predict the P of 1 spore initiating growth and toxigenesis by a particular day and at a particular temperature of storage. Studies involving other fish substrates are in progress.",
author = "Seppo Lindroth and Constantin Genigeorgis",
year = "1986",
doi = "10.1016/0168-1605(86)90012-7",
language = "English",
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journal = "International Journal of Food Microbiology",
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}

Probability of growth and toxin production by nonproteolytic Clostridium botulinum in rockfish stored under modified atmospheres. / Lindroth, Seppo; Genigeorgis, Constantin.

In: International Journal of Food Microbiology, Vol. 3, No. 3, 1986, p. 167 - 181.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Probability of growth and toxin production by nonproteolytic Clostridium botulinum in rockfish stored under modified atmospheres

AU - Lindroth, Seppo

AU - Genigeorgis, Constantin

PY - 1986

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N2 - The potential risk of C. botulinum growth in fresh fish stored under modified atmospheres remains unclear. Few studies have identified qualitatively certain conditions leading to toxigenesis. This paper is the First of a series attempting to quantify the effect of a variety of parameters on the probability (P) of toxigenesis by one spore in fish. The factorial design experiments included red snapper tissue homogenate inoculated with a pool of nonproteolytic spores (5 type E, 4 type B and 4 type F strains) at 7 levels (104−10−2 per 3 g sample) and incubated at 4, 8, 12, 17 and 30°C under 3 modified atmospheres (vacuum, 100% CO2 and 70% CO2+30% air) for up to 21 days. At the 100 spore/sample level the earliest time to detect toxin production at 4, 8, 12, 17 and 30°C under all modified atmospheres was > 21, 12, 9, 6 and 2 days, respectively. At the 101 spores/sample level the earliest times for the same temperatures were > 21, 9, 6, 3–6 and 1–2 days, respectively. The probability of toxigenesis was affected significantly (P < 0.005) by temperature, storage time, atmosphere×temperature, and temperature×time but not by atmosphere (P > 0.1). Using linear and logistic regression models, equations were derived which can predict the P of 1 spore initiating growth and toxigenesis by a particular day and at a particular temperature of storage. Studies involving other fish substrates are in progress.

AB - The potential risk of C. botulinum growth in fresh fish stored under modified atmospheres remains unclear. Few studies have identified qualitatively certain conditions leading to toxigenesis. This paper is the First of a series attempting to quantify the effect of a variety of parameters on the probability (P) of toxigenesis by one spore in fish. The factorial design experiments included red snapper tissue homogenate inoculated with a pool of nonproteolytic spores (5 type E, 4 type B and 4 type F strains) at 7 levels (104−10−2 per 3 g sample) and incubated at 4, 8, 12, 17 and 30°C under 3 modified atmospheres (vacuum, 100% CO2 and 70% CO2+30% air) for up to 21 days. At the 100 spore/sample level the earliest time to detect toxin production at 4, 8, 12, 17 and 30°C under all modified atmospheres was > 21, 12, 9, 6 and 2 days, respectively. At the 101 spores/sample level the earliest times for the same temperatures were > 21, 9, 6, 3–6 and 1–2 days, respectively. The probability of toxigenesis was affected significantly (P < 0.005) by temperature, storage time, atmosphere×temperature, and temperature×time but not by atmosphere (P > 0.1). Using linear and logistic regression models, equations were derived which can predict the P of 1 spore initiating growth and toxigenesis by a particular day and at a particular temperature of storage. Studies involving other fish substrates are in progress.

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