Probiotic and milk technological properties of Lactobacillus brevis

Elina Rönkä, Erja Malinen, Maria Saarela, Merja Rinta-Koski, Johannes Aarnikunnas, Airi Palva (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

100 Citations (Scopus)

Abstract

Two Lactobacillus brevis strains ATCC 8287 and ATCC 14869T, were evaluated for their applicability as putative probiotics in dairy products. The strains expressed good in vitro adherence to human Caco-2 and Intestine 407 cells and tolerated well low pH, bile acids and pancreatic fluid under in vitro conditions. In antimicrobial activity assays, strain ATCC 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against Bacillus cereus. Both L. brevis strains were resistant to vancomycin, which is typical for the genus Lactobacillus. The L. brevis strains were not able to acidify milk to yoghurt but were suitable as supplement strains in yoghurts. This was shown by producing a set of yoghurt products and analysing their rheological and sensory properties during a cold storage period of 28 days. Survival of the strains through human intestine was examined in 1-week feeding trials. Despite its human origin, L. brevis ATCC 14869T could not survive through the gastrointestinal (GI) tract, whereas L. brevis ATCC 8287 was detected in the faecal samples taken during and immediately after ingestion of the strain. In conclusion, L. brevis ATCC 8287 is a promising candidate as a probiotic supplement in dairy products.
Original languageEnglish
Pages (from-to)63-74
Number of pages12
JournalInternational Journal of Food Microbiology
Volume83
Issue number1
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactobacillus brevis
Probiotics
probiotics
Milk
milk
Yogurt
Dairy Products
yogurt
Intestines
dairy products
intestines
Bacillus cereus
Lactobacillus
Vancomycin
Bile Acids and Salts
vancomycin
Gastrointestinal Tract
bile acids
Eating
rheological properties

Keywords

  • Milk
  • Probiotic supplement
  • Lactobacillus brevis

Cite this

Rönkä, E., Malinen, E., Saarela, M., Rinta-Koski, M., Aarnikunnas, J., & Palva, A. (2003). Probiotic and milk technological properties of Lactobacillus brevis. International Journal of Food Microbiology, 83(1), 63-74. https://doi.org/10.1016/S0168-1605(02)00315-X
Rönkä, Elina ; Malinen, Erja ; Saarela, Maria ; Rinta-Koski, Merja ; Aarnikunnas, Johannes ; Palva, Airi. / Probiotic and milk technological properties of Lactobacillus brevis. In: International Journal of Food Microbiology. 2003 ; Vol. 83, No. 1. pp. 63-74.
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abstract = "Two Lactobacillus brevis strains ATCC 8287 and ATCC 14869T, were evaluated for their applicability as putative probiotics in dairy products. The strains expressed good in vitro adherence to human Caco-2 and Intestine 407 cells and tolerated well low pH, bile acids and pancreatic fluid under in vitro conditions. In antimicrobial activity assays, strain ATCC 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against Bacillus cereus. Both L. brevis strains were resistant to vancomycin, which is typical for the genus Lactobacillus. The L. brevis strains were not able to acidify milk to yoghurt but were suitable as supplement strains in yoghurts. This was shown by producing a set of yoghurt products and analysing their rheological and sensory properties during a cold storage period of 28 days. Survival of the strains through human intestine was examined in 1-week feeding trials. Despite its human origin, L. brevis ATCC 14869T could not survive through the gastrointestinal (GI) tract, whereas L. brevis ATCC 8287 was detected in the faecal samples taken during and immediately after ingestion of the strain. In conclusion, L. brevis ATCC 8287 is a promising candidate as a probiotic supplement in dairy products.",
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Rönkä, E, Malinen, E, Saarela, M, Rinta-Koski, M, Aarnikunnas, J & Palva, A 2003, 'Probiotic and milk technological properties of Lactobacillus brevis', International Journal of Food Microbiology, vol. 83, no. 1, pp. 63-74. https://doi.org/10.1016/S0168-1605(02)00315-X

Probiotic and milk technological properties of Lactobacillus brevis. / Rönkä, Elina; Malinen, Erja; Saarela, Maria; Rinta-Koski, Merja; Aarnikunnas, Johannes; Palva, Airi (Corresponding Author).

In: International Journal of Food Microbiology, Vol. 83, No. 1, 2003, p. 63-74.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Probiotic and milk technological properties of Lactobacillus brevis

AU - Rönkä, Elina

AU - Malinen, Erja

AU - Saarela, Maria

AU - Rinta-Koski, Merja

AU - Aarnikunnas, Johannes

AU - Palva, Airi

PY - 2003

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N2 - Two Lactobacillus brevis strains ATCC 8287 and ATCC 14869T, were evaluated for their applicability as putative probiotics in dairy products. The strains expressed good in vitro adherence to human Caco-2 and Intestine 407 cells and tolerated well low pH, bile acids and pancreatic fluid under in vitro conditions. In antimicrobial activity assays, strain ATCC 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against Bacillus cereus. Both L. brevis strains were resistant to vancomycin, which is typical for the genus Lactobacillus. The L. brevis strains were not able to acidify milk to yoghurt but were suitable as supplement strains in yoghurts. This was shown by producing a set of yoghurt products and analysing their rheological and sensory properties during a cold storage period of 28 days. Survival of the strains through human intestine was examined in 1-week feeding trials. Despite its human origin, L. brevis ATCC 14869T could not survive through the gastrointestinal (GI) tract, whereas L. brevis ATCC 8287 was detected in the faecal samples taken during and immediately after ingestion of the strain. In conclusion, L. brevis ATCC 8287 is a promising candidate as a probiotic supplement in dairy products.

AB - Two Lactobacillus brevis strains ATCC 8287 and ATCC 14869T, were evaluated for their applicability as putative probiotics in dairy products. The strains expressed good in vitro adherence to human Caco-2 and Intestine 407 cells and tolerated well low pH, bile acids and pancreatic fluid under in vitro conditions. In antimicrobial activity assays, strain ATCC 8287 showed inhibitory properties toward selected potential harmful microorganisms, particularly against Bacillus cereus. Both L. brevis strains were resistant to vancomycin, which is typical for the genus Lactobacillus. The L. brevis strains were not able to acidify milk to yoghurt but were suitable as supplement strains in yoghurts. This was shown by producing a set of yoghurt products and analysing their rheological and sensory properties during a cold storage period of 28 days. Survival of the strains through human intestine was examined in 1-week feeding trials. Despite its human origin, L. brevis ATCC 14869T could not survive through the gastrointestinal (GI) tract, whereas L. brevis ATCC 8287 was detected in the faecal samples taken during and immediately after ingestion of the strain. In conclusion, L. brevis ATCC 8287 is a promising candidate as a probiotic supplement in dairy products.

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KW - Lactobacillus brevis

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