Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

Anna Oksaharju (Corresponding Author), Matti Kankainen, Riina A. Kekkonen, Ken A. Lindstedt, Petri T. Kovanen, Riitta Korpela, Minja Miettinen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.

METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG®), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.

RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.

CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.

Original languageEnglish
Pages (from-to)750-759
JournalWorld Journal of Gastroenterology
Volume17
Issue number6
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactobacillus rhamnosus
Probiotics
Mast Cells
Down-Regulation
IgE Receptors
Histamine Receptors
Microarray Analysis
Bacteria
Gene Expression
Hypersensitivity
Gorilla gorilla
Genes
Human Genome
Interleukin-8
Interleukin-10
Immune System
Proteins
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha

Keywords

  • Probiotic bacteria
  • mast cells
  • microarray
  • allergy
  • IgE receptor

Cite this

Oksaharju, A., Kankainen, M., Kekkonen, R. A., Lindstedt, K. A., Kovanen, P. T., Korpela, R., & Miettinen, M. (2011). Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. World Journal of Gastroenterology, 17(6), 750-759. https://doi.org/10.3748/wjg.v17.i6.750
Oksaharju, Anna ; Kankainen, Matti ; Kekkonen, Riina A. ; Lindstedt, Ken A. ; Kovanen, Petri T. ; Korpela, Riitta ; Miettinen, Minja. / Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. In: World Journal of Gastroenterology. 2011 ; Vol. 17, No. 6. pp. 750-759.
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abstract = "AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG{\circledR}), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip{\circledR} Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.",
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Oksaharju, A, Kankainen, M, Kekkonen, RA, Lindstedt, KA, Kovanen, PT, Korpela, R & Miettinen, M 2011, 'Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells', World Journal of Gastroenterology, vol. 17, no. 6, pp. 750-759. https://doi.org/10.3748/wjg.v17.i6.750

Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells. / Oksaharju, Anna (Corresponding Author); Kankainen, Matti; Kekkonen, Riina A.; Lindstedt, Ken A.; Kovanen, Petri T.; Korpela, Riitta; Miettinen, Minja.

In: World Journal of Gastroenterology, Vol. 17, No. 6, 2011, p. 750-759.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

AU - Oksaharju, Anna

AU - Kankainen, Matti

AU - Kekkonen, Riina A.

AU - Lindstedt, Ken A.

AU - Kovanen, Petri T.

AU - Korpela, Riitta

AU - Miettinen, Minja

PY - 2011

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N2 - AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG®), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.

AB - AIM: To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS: Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG®), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS: LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G, respectively) and histamine H4 receptor. LGG, Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation, and on several genes that encoded proteins with a pro-inflammatory impact, such as interleukin (IL)-8 and tumour necrosis factor alpha. Also genes that encoded proteins with anti-inflammatory functions, such as IL-10, were upregulated.CONCLUSION: Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes, and by inducing a pro-inflammatory response.

KW - Probiotic bacteria

KW - mast cells

KW - microarray

KW - allergy

KW - IgE receptor

U2 - 10.3748/wjg.v17.i6.750

DO - 10.3748/wjg.v17.i6.750

M3 - Article

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SP - 750

EP - 759

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

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ER -