Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler

Dissertation

Merja Itävaara

Research output: ThesisDissertationCollection of Articles

Abstract

Different cultivation methods were compared in order to determine the best method for the large-scale production of shiitake liquid spawn. Liquid spawn has several advantages which include easy and efficient inoculation into the commercial sawdust medium. Production of the liquid spawn is also faster than growing sawdust or grain spawn. Fermenter spawn was the most suitable for large-scale production and adapted well in the supplemented sawdust medium. The method is non-labourous, and vigorous spawn is produced in only 3-5 days. The drawbacks of liquid spawn are a tendency for degeneration and mutation, after repeated subculturing. Genetic changes in the spawn are difficult to detect before fruiting. Therefore, methods for identifing L. edodes strains and species were developed. Esterase and total protein profiles of the vegetative hyphae were used to identify the strains and also to detect any possible changes in them. Either L. edodes strains or different species of wood-rotting fungi were characterized by changing the pH gradient of the gel in isoelectric focusing. A non-toxic stain ABTS was introduced and used to detect laccase activity during the growth of L. edodes mycelium in the liquid and agar medium, wood or sawdust. A spontaneous shiitake mutant strain appeared after repeated subculturing of the normal strain. The normal strain (2105) was one of the best commercially produced strains with good quality of the fruiting bodies and high yield production. The mutant strain (2105D) produced abnormal fruiting bodies in the supplemented sawdust medium, and the total yield of fruiting bodies decreased. Characteristics of the mutant were compared to those of the normal strain. Abnormal fruiting was found to result from changes in carbohydrate and protein metabolism. There were several qualitative and quantitative differences in the intra- and extracellular enzymes of the vegetative mycelium in the mutant and the normal strain. Furthermore, utilization of the carbohydrate compounds in the medium was different from that of the normal strain.
Original languageEnglish
QualificationDoctor Degree
Awarding Institution
  • University of Helsinki
Supervisors/Advisors
  • Niku-Paavola, Marja-Leena, Advisor, External person
Award date20 Aug 1993
Place of PublicationEspoo
Publisher
Print ISBNs951-38-4388-2
Publication statusPublished - 1993
MoE publication typeG5 Doctoral dissertation (article)

Fingerprint

Lentinula edodes
liquids
sawdust
fruiting bodies
mutants
mycelium
fruiting
laccase
fermenters
isoelectric focusing
carbohydrate metabolism
protein metabolism
methodology
esterases
hyphae
agar
gels

Keywords

  • shiitake
  • Lentinula edodes
  • liquid cultivation
  • mutant
  • abnormal fruiting

Cite this

Itävaara, M. (1993). Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler: Dissertation. Espoo: VTT Technical Research Centre of Finland.
Itävaara, Merja. / Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler : Dissertation. Espoo : VTT Technical Research Centre of Finland, 1993. 114 p.
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abstract = "Different cultivation methods were compared in order to determine the best method for the large-scale production of shiitake liquid spawn. Liquid spawn has several advantages which include easy and efficient inoculation into the commercial sawdust medium. Production of the liquid spawn is also faster than growing sawdust or grain spawn. Fermenter spawn was the most suitable for large-scale production and adapted well in the supplemented sawdust medium. The method is non-labourous, and vigorous spawn is produced in only 3-5 days. The drawbacks of liquid spawn are a tendency for degeneration and mutation, after repeated subculturing. Genetic changes in the spawn are difficult to detect before fruiting. Therefore, methods for identifing L. edodes strains and species were developed. Esterase and total protein profiles of the vegetative hyphae were used to identify the strains and also to detect any possible changes in them. Either L. edodes strains or different species of wood-rotting fungi were characterized by changing the pH gradient of the gel in isoelectric focusing. A non-toxic stain ABTS was introduced and used to detect laccase activity during the growth of L. edodes mycelium in the liquid and agar medium, wood or sawdust. A spontaneous shiitake mutant strain appeared after repeated subculturing of the normal strain. The normal strain (2105) was one of the best commercially produced strains with good quality of the fruiting bodies and high yield production. The mutant strain (2105D) produced abnormal fruiting bodies in the supplemented sawdust medium, and the total yield of fruiting bodies decreased. Characteristics of the mutant were compared to those of the normal strain. Abnormal fruiting was found to result from changes in carbohydrate and protein metabolism. There were several qualitative and quantitative differences in the intra- and extracellular enzymes of the vegetative mycelium in the mutant and the normal strain. Furthermore, utilization of the carbohydrate compounds in the medium was different from that of the normal strain.",
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Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler : Dissertation. / Itävaara, Merja.

Espoo : VTT Technical Research Centre of Finland, 1993. 114 p.

Research output: ThesisDissertationCollection of Articles

TY - THES

T1 - Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler

T2 - Dissertation

AU - Itävaara, Merja

N1 - Project code: ELI2813

PY - 1993

Y1 - 1993

N2 - Different cultivation methods were compared in order to determine the best method for the large-scale production of shiitake liquid spawn. Liquid spawn has several advantages which include easy and efficient inoculation into the commercial sawdust medium. Production of the liquid spawn is also faster than growing sawdust or grain spawn. Fermenter spawn was the most suitable for large-scale production and adapted well in the supplemented sawdust medium. The method is non-labourous, and vigorous spawn is produced in only 3-5 days. The drawbacks of liquid spawn are a tendency for degeneration and mutation, after repeated subculturing. Genetic changes in the spawn are difficult to detect before fruiting. Therefore, methods for identifing L. edodes strains and species were developed. Esterase and total protein profiles of the vegetative hyphae were used to identify the strains and also to detect any possible changes in them. Either L. edodes strains or different species of wood-rotting fungi were characterized by changing the pH gradient of the gel in isoelectric focusing. A non-toxic stain ABTS was introduced and used to detect laccase activity during the growth of L. edodes mycelium in the liquid and agar medium, wood or sawdust. A spontaneous shiitake mutant strain appeared after repeated subculturing of the normal strain. The normal strain (2105) was one of the best commercially produced strains with good quality of the fruiting bodies and high yield production. The mutant strain (2105D) produced abnormal fruiting bodies in the supplemented sawdust medium, and the total yield of fruiting bodies decreased. Characteristics of the mutant were compared to those of the normal strain. Abnormal fruiting was found to result from changes in carbohydrate and protein metabolism. There were several qualitative and quantitative differences in the intra- and extracellular enzymes of the vegetative mycelium in the mutant and the normal strain. Furthermore, utilization of the carbohydrate compounds in the medium was different from that of the normal strain.

AB - Different cultivation methods were compared in order to determine the best method for the large-scale production of shiitake liquid spawn. Liquid spawn has several advantages which include easy and efficient inoculation into the commercial sawdust medium. Production of the liquid spawn is also faster than growing sawdust or grain spawn. Fermenter spawn was the most suitable for large-scale production and adapted well in the supplemented sawdust medium. The method is non-labourous, and vigorous spawn is produced in only 3-5 days. The drawbacks of liquid spawn are a tendency for degeneration and mutation, after repeated subculturing. Genetic changes in the spawn are difficult to detect before fruiting. Therefore, methods for identifing L. edodes strains and species were developed. Esterase and total protein profiles of the vegetative hyphae were used to identify the strains and also to detect any possible changes in them. Either L. edodes strains or different species of wood-rotting fungi were characterized by changing the pH gradient of the gel in isoelectric focusing. A non-toxic stain ABTS was introduced and used to detect laccase activity during the growth of L. edodes mycelium in the liquid and agar medium, wood or sawdust. A spontaneous shiitake mutant strain appeared after repeated subculturing of the normal strain. The normal strain (2105) was one of the best commercially produced strains with good quality of the fruiting bodies and high yield production. The mutant strain (2105D) produced abnormal fruiting bodies in the supplemented sawdust medium, and the total yield of fruiting bodies decreased. Characteristics of the mutant were compared to those of the normal strain. Abnormal fruiting was found to result from changes in carbohydrate and protein metabolism. There were several qualitative and quantitative differences in the intra- and extracellular enzymes of the vegetative mycelium in the mutant and the normal strain. Furthermore, utilization of the carbohydrate compounds in the medium was different from that of the normal strain.

KW - shiitake

KW - Lentinula edodes

KW - liquid cultivation

KW - mutant

KW - abnormal fruiting

M3 - Dissertation

SN - 951-38-4388-2

T3 - VTT Publications

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Itävaara M. Problems associated with the liquid cultivation of shiitake, Lentinula edodes (Berk.) Pegler: Dissertation. Espoo: VTT Technical Research Centre of Finland, 1993. 114 p.