Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo; Lasse Fredriksen; Maren Oftebro; Magnus Arntzen; Olav A. Aarstad; Runar Stokke; Ida H. Steen; Line Degn Hansen; Reidar B. Schüller; Finn L. Aachmann; Svein J. Horn; Vincent G.H. Eijsink

doi:10.1021/acs.jafc.8b07190

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo, Lasse Fredriksen, Maren Oftebro, Magnus Arntzen, Olav A. Aarstad, Runar Stokke, Ida H. Steen, Line Degn Hansen, Reidar B. Schüller, Finn L. Aachmann, Svein J. Horn, Vincent G.H. Eijsink

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Research output: Contribution to journal › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k _cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s ^-1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

Original language	English
Pages (from-to)	2936-2945
Number of pages	10
Journal	Journal of Agricultural and Food Chemistry
Volume	67
Issue number	10
DOIs	https://doi.org/10.1021/acs.jafc.8b07190
Publication status	Published - 13 Mar 2019
MoE publication type	A1 Journal article-refereed

Funding

This research was supported by the Research Council of Norway through grants 229003 (BIOFEEDNovel salmon feed by integrated bioprocessing of nonfood biomass), 237841 (Foods of Norway), and 221568 (NorZymeD). Infrastructure was supported in part by NorBioLab grants 226247 and 270038 provided by the Research Council of Norway. The authors would like to thank Marianne Slang-Jensen for advice in the studies of protein stability.

Keywords

alginate lyase
biorefining
brown seaweed
PolyM
Saccharina latissima
salt tolerance
Metagenomics
Temperature
Biocatalysis
Bacteria/classification
Hydrothermal Vents/chemistry
Enzyme Stability
Polysaccharide-Lyases/chemistry
Seaweed/chemistry
Substrate Specificity
Phaeophyceae/chemistry
Phylogeny
Polysaccharides/chemistry
Arctic Regions
Bacterial Proteins/chemistry
Kinetics
Hydrogen-Ion Concentration

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10.1021/acs.jafc.8b07190

Cite this

Vuoristo, K. S., Fredriksen, L., Oftebro, M., Arntzen, M., Aarstad, O. A., Stokke, R., Steen, I. H., Hansen, L. D., Schüller, R. B., Aachmann, F. L., Horn, S. J., & Eijsink, V. G. H. (2019). Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. Journal of Agricultural and Food Chemistry, 67(10), 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190

@article{756746a01bfc4959b9904084f334652b,

title = "Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge",

abstract = " Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing. ",

keywords = "alginate lyase, biorefining, brown seaweed, PolyM, Saccharina latissima, salt tolerance, Metagenomics, Temperature, Biocatalysis, Bacteria/classification, Hydrothermal Vents/chemistry, Enzyme Stability, Polysaccharide-Lyases/chemistry, Seaweed/chemistry, Substrate Specificity, Phaeophyceae/chemistry, Phylogeny, Polysaccharides/chemistry, Arctic Regions, Bacterial Proteins/chemistry, Kinetics, Hydrogen-Ion Concentration",

author = "Vuoristo, {Kiira S.} and Lasse Fredriksen and Maren Oftebro and Magnus Arntzen and Aarstad, {Olav A.} and Runar Stokke and Steen, {Ida H.} and Hansen, {Line Degn} and Sch{\"u}ller, {Reidar B.} and Aachmann, {Finn L.} and Horn, {Svein J.} and Eijsink, {Vincent G.H.}",

year = "2019",

month = mar,

day = "13",

doi = "10.1021/acs.jafc.8b07190",

language = "English",

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pages = "2936--2945",

journal = "Journal of Agricultural and Food Chemistry",

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Vuoristo, KS, Fredriksen, L, Oftebro, M, Arntzen, M, Aarstad, OA, Stokke, R, Steen, IH, Hansen, LD, Schüller, RB, Aachmann, FL, Horn, SJ & Eijsink, VGH 2019, 'Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge', Journal of Agricultural and Food Chemistry, vol. 67, no. 10, pp. 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190

TY - JOUR

T1 - Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

AU - Vuoristo, Kiira S.

AU - Fredriksen, Lasse

AU - Oftebro, Maren

AU - Arntzen, Magnus

AU - Aarstad, Olav A.

AU - Stokke, Runar

AU - Steen, Ida H.

AU - Hansen, Line Degn

AU - Schüller, Reidar B.

AU - Aachmann, Finn L.

AU - Horn, Svein J.

AU - Eijsink, Vincent G.H.

PY - 2019/3/13

Y1 - 2019/3/13

N2 - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

AB - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

KW - alginate lyase

KW - biorefining

KW - brown seaweed

KW - PolyM

KW - Saccharina latissima

KW - salt tolerance

KW - Metagenomics

KW - Temperature

KW - Biocatalysis

KW - Bacteria/classification

KW - Hydrothermal Vents/chemistry

KW - Enzyme Stability

KW - Polysaccharide-Lyases/chemistry

KW - Seaweed/chemistry

KW - Substrate Specificity

KW - Phaeophyceae/chemistry

KW - Phylogeny

KW - Polysaccharides/chemistry

KW - Arctic Regions

KW - Bacterial Proteins/chemistry

KW - Kinetics

KW - Hydrogen-Ion Concentration

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U2 - 10.1021/acs.jafc.8b07190

DO - 10.1021/acs.jafc.8b07190

M3 - Article

C2 - 30781951

AN - SCOPUS:85062561696

SN - 0021-8561

VL - 67

SP - 2936

EP - 2945

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 10

ER -

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Abstract

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Keywords

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