Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo; Lasse Fredriksen; Maren Oftebro; Magnus Arntzen; Olav A. Aarstad; Runar Stokke; Ida H. Steen; Line Degn Hansen; Reidar B. Schüller; Finn L. Aachmann; Svein J. Horn; Vincent G.H. Eijsink

doi:10.1021/acs.jafc.8b07190

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo, Lasse Fredriksen, Maren Oftebro, Magnus Arntzen, Olav A. Aarstad, Runar Stokke, Ida H. Steen, Line Degn Hansen, Reidar B. Schüller, Finn L. Aachmann, Svein J. Horn, Vincent G.H. Eijsink

Research output: Contribution to journal › Article › Scientific › peer-review

8 Citations (Scopus)

Abstract

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k _cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s ^-1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

Original language	English
Pages (from-to)	2936-2945
Number of pages	10
Journal	Journal of Agricultural and Food Chemistry
Volume	67
Issue number	10
DOIs	https://doi.org/10.1021/acs.jafc.8b07190
Publication status	Published - 13 Mar 2019
MoE publication type	A1 Journal article-refereed

Keywords

alginate lyase
biorefining
brown seaweed
PolyM
Saccharina latissima
salt tolerance

Access to Document

10.1021/acs.jafc.8b07190

Fingerprint Dive into the research topics of 'Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge'. Together they form a unique fingerprint.

Cite this

Vuoristo, K. S., Fredriksen, L., Oftebro, M., Arntzen, M., Aarstad, O. A., Stokke, R., Steen, I. H., Hansen, L. D., Schüller, R. B., Aachmann, F. L., Horn, S. J., & Eijsink, V. G. H. (2019). Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. Journal of Agricultural and Food Chemistry, 67(10), 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190

Vuoristo, Kiira S. ; Fredriksen, Lasse ; Oftebro, Maren ; Arntzen, Magnus ; Aarstad, Olav A. ; Stokke, Runar ; Steen, Ida H. ; Hansen, Line Degn ; Schüller, Reidar B. ; Aachmann, Finn L. ; Horn, Svein J. ; Eijsink, Vincent G.H. / Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. In: Journal of Agricultural and Food Chemistry. 2019 ; Vol. 67, No. 10. pp. 2936-2945.

@article{756746a01bfc4959b9904084f334652b,

title = "Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge",

abstract = " Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing. ",

keywords = "alginate lyase, biorefining, brown seaweed, PolyM, Saccharina latissima, salt tolerance",

author = "Vuoristo, {Kiira S.} and Lasse Fredriksen and Maren Oftebro and Magnus Arntzen and Aarstad, {Olav A.} and Runar Stokke and Steen, {Ida H.} and Hansen, {Line Degn} and Sch{\"u}ller, {Reidar B.} and Aachmann, {Finn L.} and Horn, {Svein J.} and Eijsink, {Vincent G.H.}",

year = "2019",

month = mar,

day = "13",

doi = "10.1021/acs.jafc.8b07190",

language = "English",

volume = "67",

pages = "2936--2945",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society ACS",

number = "10",

}

Vuoristo, KS, Fredriksen, L, Oftebro, M, Arntzen, M, Aarstad, OA, Stokke, R, Steen, IH, Hansen, LD, Schüller, RB, Aachmann, FL, Horn, SJ & Eijsink, VGH 2019, 'Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge', Journal of Agricultural and Food Chemistry, vol. 67, no. 10, pp. 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. / Vuoristo, Kiira S.; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus; Aarstad, Olav A.; Stokke, Runar; Steen, Ida H.; Hansen, Line Degn; Schüller, Reidar B.; Aachmann, Finn L.; Horn, Svein J.; Eijsink, Vincent G.H.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 10, 13.03.2019, p. 2936-2945.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

AU - Vuoristo, Kiira S.

AU - Fredriksen, Lasse

AU - Oftebro, Maren

AU - Arntzen, Magnus

AU - Aarstad, Olav A.

AU - Stokke, Runar

AU - Steen, Ida H.

AU - Hansen, Line Degn

AU - Schüller, Reidar B.

AU - Aachmann, Finn L.

AU - Horn, Svein J.

AU - Eijsink, Vincent G.H.

PY - 2019/3/13

Y1 - 2019/3/13

N2 - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

AB - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

KW - alginate lyase

KW - biorefining

KW - brown seaweed

KW - PolyM

KW - Saccharina latissima

KW - salt tolerance

UR - http://www.scopus.com/inward/record.url?scp=85062561696&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.8b07190

DO - 10.1021/acs.jafc.8b07190

M3 - Article

C2 - 30781951

AN - SCOPUS:85062561696

VL - 67

SP - 2936

EP - 2945

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 10

ER -

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Abstract

Keywords

Access to Document

Other files and links

Cite this