Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo, Lasse Fredriksen, Maren Oftebro, Magnus Arntzen, Olav A. Aarstad, Runar Stokke, Ida H. Steen, Line Degn Hansen, Reidar B. Schüller, Finn L. Aachmann, Svein J. Horn, Vincent G.H. Eijsink

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

Original languageEnglish
Pages (from-to)2936-2945
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number10
DOIs
Publication statusPublished - 13 Mar 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • alginate lyase
  • biorefining
  • brown seaweed
  • PolyM
  • Saccharina latissima
  • salt tolerance

Fingerprint Dive into the research topics of 'Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge'. Together they form a unique fingerprint.

  • Cite this

    Vuoristo, K. S., Fredriksen, L., Oftebro, M., Arntzen, M., Aarstad, O. A., Stokke, R., Steen, I. H., Hansen, L. D., Schüller, R. B., Aachmann, F. L., Horn, S. J., & Eijsink, V. G. H. (2019). Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. Journal of Agricultural and Food Chemistry, 67(10), 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190