Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

Kiira S. Vuoristo, Lasse Fredriksen, Maren Oftebro, Magnus Arntzen, Olav A. Aarstad, Runar Stokke, Ida H. Steen, Line Degn Hansen, Reidar B. Schüller, Finn L. Aachmann, Svein J. Horn, Vincent G.H. Eijsink

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

Original languageEnglish
Pages (from-to)2936-2945
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number10
DOIs
Publication statusPublished - 13 Mar 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

Seaweed
Vents
Oceans and Seas
macroalgae
Arctic region
lyases
oceans
polysaccharides
Polysaccharide-Lyases
sodium alginate
Phaeophyceae
depolymerization
alginates
endo-1,4-beta-glucanase
oligosaccharides
Depolymerization
Lyases
Cellulase
Salinity
Seawater

Keywords

  • alginate lyase
  • biorefining
  • brown seaweed
  • PolyM
  • Saccharina latissima
  • salt tolerance

Cite this

Vuoristo, K. S., Fredriksen, L., Oftebro, M., Arntzen, M., Aarstad, O. A., Stokke, R., ... Eijsink, V. G. H. (2019). Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. Journal of Agricultural and Food Chemistry, 67(10), 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190
Vuoristo, Kiira S. ; Fredriksen, Lasse ; Oftebro, Maren ; Arntzen, Magnus ; Aarstad, Olav A. ; Stokke, Runar ; Steen, Ida H. ; Hansen, Line Degn ; Schüller, Reidar B. ; Aachmann, Finn L. ; Horn, Svein J. ; Eijsink, Vincent G.H. / Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. In: Journal of Agricultural and Food Chemistry. 2019 ; Vol. 67, No. 10. pp. 2936-2945.
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abstract = "Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.",
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author = "Vuoristo, {Kiira S.} and Lasse Fredriksen and Maren Oftebro and Magnus Arntzen and Aarstad, {Olav A.} and Runar Stokke and Steen, {Ida H.} and Hansen, {Line Degn} and Sch{\"u}ller, {Reidar B.} and Aachmann, {Finn L.} and Horn, {Svein J.} and Eijsink, {Vincent G.H.}",
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Vuoristo, KS, Fredriksen, L, Oftebro, M, Arntzen, M, Aarstad, OA, Stokke, R, Steen, IH, Hansen, LD, Schüller, RB, Aachmann, FL, Horn, SJ & Eijsink, VGH 2019, 'Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge', Journal of Agricultural and Food Chemistry, vol. 67, no. 10, pp. 2936-2945. https://doi.org/10.1021/acs.jafc.8b07190

Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. / Vuoristo, Kiira S.; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus; Aarstad, Olav A.; Stokke, Runar; Steen, Ida H.; Hansen, Line Degn; Schüller, Reidar B.; Aachmann, Finn L.; Horn, Svein J.; Eijsink, Vincent G.H.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 10, 13.03.2019, p. 2936-2945.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

AU - Vuoristo, Kiira S.

AU - Fredriksen, Lasse

AU - Oftebro, Maren

AU - Arntzen, Magnus

AU - Aarstad, Olav A.

AU - Stokke, Runar

AU - Steen, Ida H.

AU - Hansen, Line Degn

AU - Schüller, Reidar B.

AU - Aachmann, Finn L.

AU - Horn, Svein J.

AU - Eijsink, Vincent G.H.

PY - 2019/3/13

Y1 - 2019/3/13

N2 - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

AB - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.

KW - alginate lyase

KW - biorefining

KW - brown seaweed

KW - PolyM

KW - Saccharina latissima

KW - salt tolerance

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