Abstract
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.
| Original language | English |
|---|---|
| Pages (from-to) | 2936-2945 |
| Number of pages | 10 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 67 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 13 Mar 2019 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- alginate lyase
- biorefining
- brown seaweed
- PolyM
- Saccharina latissima
- salt tolerance
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}
Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge. / Vuoristo, Kiira S.; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus; Aarstad, Olav A.; Stokke, Runar; Steen, Ida H.; Hansen, Line Degn; Schüller, Reidar B.; Aachmann, Finn L.; Horn, Svein J.; Eijsink, Vincent G.H.
In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 10, 13.03.2019, p. 2936-2945.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Production, Characterization, and Application of an Alginate Lyase, AMOR-PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge
AU - Vuoristo, Kiira S.
AU - Fredriksen, Lasse
AU - Oftebro, Maren
AU - Arntzen, Magnus
AU - Aarstad, Olav A.
AU - Stokke, Runar
AU - Steen, Ida H.
AU - Hansen, Line Degn
AU - Schüller, Reidar B.
AU - Aachmann, Finn L.
AU - Horn, Svein J.
AU - Eijsink, Vincent G.H.
PY - 2019/3/13
Y1 - 2019/3/13
N2 - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.
AB - Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.
KW - alginate lyase
KW - biorefining
KW - brown seaweed
KW - PolyM
KW - Saccharina latissima
KW - salt tolerance
UR - http://www.scopus.com/inward/record.url?scp=85062561696&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.8b07190
DO - 10.1021/acs.jafc.8b07190
M3 - Article
C2 - 30781951
AN - SCOPUS:85062561696
VL - 67
SP - 2936
EP - 2945
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 10
ER -