Abstract
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR-PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR-PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR-PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.
Original language | English |
---|---|
Pages (from-to) | 2936-2945 |
Number of pages | 10 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 67 |
Issue number | 10 |
DOIs | |
Publication status | Published - 13 Mar 2019 |
MoE publication type | A1 Journal article-refereed |
Keywords
- alginate lyase
- biorefining
- brown seaweed
- PolyM
- Saccharina latissima
- salt tolerance